ID   RPOB_CHRSD              Reviewed;        1358 AA.
AC   Q1R0I2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Csal_0414;
OS   Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 /
OS   NCIMB 13768 / 1H11).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC   Halomonadaceae; Chromohalobacter.
OX   NCBI_TaxID=290398;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11;
RX   PubMed=22675587; DOI=10.4056/sigs.2285059;
RA   Copeland A., O'Connor K., Lucas S., Lapidus A., Berry K.W., Detter J.C.,
RA   Del Rio T.G., Hammon N., Dalin E., Tice H., Pitluck S., Bruce D.,
RA   Goodwin L., Han C., Tapia R., Saunders E., Schmutz J., Brettin T.,
RA   Larimer F., Land M., Hauser L., Vargas C., Nieto J.J., Kyrpides N.C.,
RA   Ivanova N., Goker M., Klenk H.P., Csonka L.N., Woyke T.;
RT   "Complete genome sequence of the halophilic and highly halotolerant
RT   Chromohalobacter salexigens type strain (1H11(T)).";
RL   Stand. Genomic Sci. 5:379-388(2011).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000285; ABE57776.1; -; Genomic_DNA.
DR   RefSeq; WP_011505722.1; NC_007963.1.
DR   AlphaFoldDB; Q1R0I2; -.
DR   SMR; Q1R0I2; -.
DR   STRING; 290398.Csal_0414; -.
DR   PRIDE; Q1R0I2; -.
DR   EnsemblBacteria; ABE57776; ABE57776; Csal_0414.
DR   KEGG; csa:Csal_0414; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000239; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1358
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300299"
SQ   SEQUENCE   1358 AA;  151639 MW;  017A9F0F8E3F8223 CRC64;
     MAYSYTEKKR IRKDFGKLPQ VMDVPYLLAI QLDSYFDFLQ QDRAIEERLD VGLHAAFKSV
     FPIESFSGNA ALEYVSYRFG TPAFDVKECQ LRGVTYSAPL RVKVRLIIYD KDSSTKAIKD
     IKEQEVYMGE IPLMTENGTF VVNGTERVIV SQLHRSPGVF FDHDKGKSHS SGKLLYSARI
     IPYRGSWLDF EFDPKDNVYV RIDRRRKLPA TVLLRALGMS AEEILETFFD TSTFHIEKSG
     FSVELVPSRL RGETATFDIK DGEGNLIVEE GRRITQKHIR QMEKAGLERL DVPMEYLFGK
     TLAKDQVDPN TGELVCECNT EITPELLEKI GQAGITTLET LYTNDLDTGP FVSDTLKIDT
     THSQLEALVE IYRMMRPGEP PTKEAAETLF HNLFFTEDRY DLSGVGRMKF NRRLRREGDT
     GSGVLDNQDI LDVMRELINI RNGFGEVDDI DHLGNRRIRC VGEMAENQFR VGLVRVERAV
     KERLSMAESE GLMPQDLINA KPVAAAVKEF FGSSQLSQFM DQNNPLSEVT HKRRVSALGP
     GGLTRERAGF EVRDVHATHY GRLCPIETLE GPNIGLINSL ATYSHTNSYG FLETPYRKVV
     DRQVTDEVVH LSAIEEGDFI IAQASATVDE SNKLTDDLVQ VRHKGETTFM APDKVTLMDV
     SPRQVVSVAA ALIPFLEHDD ANRALMGSNM QRQAVPTLKA EKPLVGTGME RFVARDSGVC
     AVARRGGVID SVDAKRIVVR INEDEIIGGE AGVDIYNLTK YVRSNQNTCM NQRPIVRPGD
     VVARGDILAD GPSVDMGDLA LGQNMRMAFM PWNGYNFEDS ILISERVVEE DRFTSIHIQE
     LTCVSRDTKL GAEEISSDIP NVGEAALGKL DDAGIVYIGA EVGAGDILVG KVTPKGETQL
     TPEEKLLRAI FGEKASDVKD TSLRAPTGMK GTVIDVQVFT RDGVEKDSRA LSIEQSQLDE
     VRKDLQETYR IAEEATFERL KRALIGQPVN GGPKLKKGDT LDEAYLDELP RSQWFKLRMQ
     DESLNELLAQ ADEQLENRRK EMDERFEDKK RKLTQGDDLA PGVLKIVKVY LAVKRRIQSG
     DKMAGRHGNK GVISSIMPIE DMPFDDNGEP VDVVLNPLGV PSRMNVGQIL ETHLGMAANG
     LGVKIDRMLR DARQQQVAEI RDFLGQVYNT AETRQEDIDS LSDDEIITLA KNLRSGVPVA
     TPVFDGAKEH EIKHLLTLAD LPDSGQMTLY DGRTGDAFDR QVTVGYMYML KLNHLVDDKM
     HARSTGSYSL VTQQPLGGKA QFGGQRFGEM EVWALEAYGA AYTLQEMLTV KSDDVEGRTK
     MYKNIVDGDH SMQAGMPESF NVLVKEIRSL GIDIELEG