ID   RPOB_CHRVO              Reviewed;        1390 AA.
AC   Q7NQE6;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CV_4193;
OS   Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC
OS   12614 / NCIMB 9131 / NCTC 9757).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;
OC   Chromobacteriaceae; Chromobacterium.
OX   NCBI_TaxID=243365;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC
RC   9757;
RX   PubMed=14500782; DOI=10.1073/pnas.1832124100;
RA   Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T.,
RA   Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R.,
RA   Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F.,
RA   Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S.,
RA   Belo A., van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M.,
RA   Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P.,
RA   Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S.,
RA   Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N.,
RA   Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P.,
RA   Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R.,
RA   Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B.,
RA   Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J.,
RA   Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F.,
RA   Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z.,
RA   de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F.,
RA   Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A.,
RA   Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C.,
RA   Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M.,
RA   Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C.,
RA   Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R.,
RA   Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M.,
RA   Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R.,
RA   Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G.;
RT   "The complete genome sequence of Chromobacterium violaceum reveals
RT   remarkable and exploitable bacterial adaptability.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE016825; AAQ61853.1; -; Genomic_DNA.
DR   RefSeq; WP_011137740.1; NC_005085.1.
DR   AlphaFoldDB; Q7NQE6; -.
DR   SMR; Q7NQE6; -.
DR   STRING; 243365.CV_4193; -.
DR   PRIDE; Q7NQE6; -.
DR   EnsemblBacteria; AAQ61853; AAQ61853; CV_4193.
DR   GeneID; 66366335; -.
DR   KEGG; cvi:CV_4193; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_4; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001424; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1390
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047883"
SQ   SEQUENCE   1390 AA;  155556 MW;  49D002DC3E5BD0D5 CRC64;
     MSYSFTEKKR IRKSFAKRAS VLDVPFLLAT QIDSYTEFLQ LGTPLDERKD VGLQAAFKSI
     FPIVSHNGYA RLDFAHYVLG EPPFDVQECQ LRGITFAAPL RARIRLTIFD KESSKPVVKE
     VRENEVYMGE IPLMTANGSF IINGTERVIV SQLHRSPGVF FEHDRGKTHS SGKLLFSARV
     IPYRGSWLDF EFDPKDLLYF RIDRRRKMPV TILLKALGYT NEEILSEFYS FDTFYLTKSG
     VFMRVVPERL KGEVAKFDIV AADGKLIVAK DKRITAKHIR DIQTAELDRI EVPADALLGK
     MLAHNVVNQN TGEVIARANE EVTEEILAKL ALAEVEQVEV LFTNDLDQGA YISQTLRTDD
     IQDQTQARVA IYRMMRPGEP PTEDAVEALF QRLFFSDETY DLSRVGRMKF SSRTYQYKFD
     DKTPEWFKTL IGEKFASRRD VMEGTLATED IVSVIAILTE LRNGRGEVDD IDHLGNRRVR
     SVGELAENQF RAGLVRVERA VKERLNQAES DNLMPHDLIN AKPVSAAIKE FFGSSQLSQF
     MDQTNPLSEI THKRRVSALG PGGLTRERAG FEVRDVHPTH YGRVCPIETP EGPNIGLINS
     LSVYARTNEF GFLETPYRKV VDGKVTNEID YLSAIEEGRY VIAQANAELD GEGALIDELV
     TCREKGETIL ATPDRVQYMD VATGQVVSVA ASLIPFLEHD DANRALMGAN MQRQAVPCLR
     PEKAFVGTGI ERSVAVDSGT TVVARRGGVV DYVDAGRVVV RVNDEEATAG EVGVDIYNLT
     KFTRSNQNTN INQRPVVKVG DHIARGDVVA DGASTDLGEL ALGQNMTIAF MPWNGYNYED
     SILISEKLVA EDRYTSIHIE ELSVVARDTK LGPEEITRDI PNLSERMAGR LDESGIVYIG
     AEVEAGDVLV GKVTPKGETQ LTPEEKLLRA IFGEKASDVK DTSLRVPTGT VGTVIDVQVF
     TREGIERDKR AQSIIDAELK RYRLDLNDQL RIFDNDAFSR IERLIVGKAA NGGPKRLAKG
     TVIDQEYLAG LPTKHDWFDI RMADEDIAKQ LELIKESLAQ KREEFDLKFE DKKRKLTQGD
     ELPPGVQKMV KVYLAVKRRL QAGDKMAGRH GNKGVVSRIL PVEDMPYMGD GRPVDIVLNP
     LGVPSRMNIG QILEVHLGWA AKGIGERINR MVREQSAAEI RAYLERIYNE TGKPEEIAAL
     SDAEVMQLAQ NLSKGMTFAT PVFDGAKEAE IKHMLDLAYP DGDELTEKMG FNESKTQMTL
     FDGRSGEAFD RKVTVGVMHY LKLHHLVDDK MHARSTGPYS LVTQQPLGGK AQFGGQRFGE
     MEVWALEAYG AAYTLQEMLT VKSDDVTGRT KVYENIVKGE HKIDAGMPES FNVLVKEIRS
     LGLDMDLERY