ID   RPOB_CITBB              Reviewed;        1371 AA.
AC   B5EFP3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Gbem_0926;
OS   Citrifermentans bemidjiense (strain ATCC BAA-1014 / DSM 16622 / JCM 12645 /
OS   Bem) (Geobacter bemidjiensis).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Citrifermentans.
OX   NCBI_TaxID=404380;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1014 / DSM 16622 / JCM 12645 / Bem;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Lykidis A., Lovley D., Richardson P.;
RT   "Complete sequence of Geobacter bemidjiensis BEM.";
RL   Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP001124; ACH37947.1; -; Genomic_DNA.
DR   RefSeq; WP_012529359.1; NC_011146.1.
DR   AlphaFoldDB; B5EFP3; -.
DR   SMR; B5EFP3; -.
DR   STRING; 404380.Gbem_0926; -.
DR   PRIDE; B5EFP3; -.
DR   EnsemblBacteria; ACH37947; ACH37947; Gbem_0926.
DR   KEGG; gbm:Gbem_0926; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_7; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000008825; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1371
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000141699"
SQ   SEQUENCE   1371 AA;  152913 MW;  C0C9FF0C09032CDE CRC64;
     MAYSIANNPL LRKNFAKIHK IIDIPNLIDI QKNSYKRFLQ LDTPVDARKN SGLEAVFRSV
     FPIRDFSDTA SLEYVSYSLG APKYDVEECH QRGMTFAAPM KVKVRLVVWD VAKDPGTRSI
     RDIKEQEVYF GEIPLMTDNG TFIINGTERV IVSQLHRSPG VFYDHDKGKT HSSGKVLYSA
     RVIPYRGSWL DFEFDHKDIL YVRIDRRRKM PATVLLKALG YSNDALINYF YKSEEVKLGD
     NGAMTKLADA ELLSNQKATA DIVDPATGEV ILKANRKFTK AALRKMAEHG IKEIPISEEE
     VVAKVASHDI YDPATGEIIV ECNEELTQAK LEEIIQKGIT TFQVLFIDNL HVTSSFRDTI
     LIDKIGSTDE ALIEIYRRLR PGDPPTLKSA LVLFENLFFN AERYDLSAVG RLKLNYKLGV
     DVPLDCMTLT REDILEVVRY LIELKNGKGN IDDIDHLGNR RVRAVGELLE NQYRIGLVRM
     ERAIKERMSL QEVENLMPHD LINSKPVSAV VKEFFGSSQL SQFMDQTNPL SEVTHKRRLS
     ALGPGGLTRE RAGFEVRDVH PTHYGRVCPI ETPEGPNIGL IASLSTYARI NEHGFVETPY
     RVVSEGKVTD EVRFFSALEE EGHAIAQANA EIDKDGRFAA DYISARKSGE FVLVGRDELE
     LMDVAPMQLV SVAASLIPFL ENDDANRALM GSNMQRQAVP LLRADSPLVG TGMERVVARD
     SGVSSVARHN GVVESVDASR IVVKIDEDQY DATGTGVDIY NLIKFARSNQ NTCINQRPVV
     KVGDHVKAGD IIADGPSTDM GELALGQNVL IAFMPWGGYN YEDSILISER LVKDDRYTSI
     HIEEFEAVAR DTKLGKEEIT SDIPNLGEET LKDLDESGII RIGAEVRPGD ILVGKITPKG
     ETQLSPEEKL LRAIFGEKAG DVRDTSLRVP PGVEGTVIGA KIFSRKGADK DARTEIIEKA
     EEMRLRKDEQ DEIRIIRDSA IGKLKKLLVG KAAAVKIEGS DGKVLIPKGA AITEEMLKSF
     SMDRWDEISI ADDDTIDEKV AQTLSTLNQQ IDIIKYVFDD KVQKLRRGDD LPPGVIKMVK
     VYIAIKRKLQ VGDKMAGRHG NKGVVSRILP EEDMPYMEDG RPVEIVLNPL GVPSRMNVGQ
     ILEMHLGWGA KGLGWKIEEF LEKNAPHDEI KRFLKGAYDN PEMDRFLDTL EGEELLNLAR
     RLKRGIPMSS PVFEGASEES IQSMLTHAGF STTGQVTLYD GKSGDKFMHQ VTVGIMYFLK
     LHHLVDDKIH ARSIGPYSLV TQQPLGGKAR FGGQRLGEME VWAMEAYGAA YALQEFLTVK
     SDDVAGRTRM YEAIVKGKHT LEPGLPESFN VLIKELQSLG LDVELLEGDE D