ID   RPOB_CITSI              Reviewed;        1070 AA.
AC   Q09MI6;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX   NCBI_TaxID=2711;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Osbeck var. Ridge Pineapple;
RX   PubMed=17010212; DOI=10.1186/1471-2229-6-21;
RA   Bausher M.G., Singh N.D., Lee S.-B., Jansen R.K., Daniell H.;
RT   "The complete chloroplast genome sequence of Citrus sinensis (L.) Osbeck
RT   var 'Ridge Pineapple': organization and phylogenetic relationships to other
RT   angiosperms.";
RL   BMC Plant Biol. 6:21-21(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; DQ864733; ABI49012.1; -; Genomic_DNA.
DR   RefSeq; YP_740467.1; NC_008334.1.
DR   AlphaFoldDB; Q09MI6; -.
DR   SMR; Q09MI6; -.
DR   GeneID; 4271235; -.
DR   KEGG; cit:4271235; -.
DR   OrthoDB; 944344at2759; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1070
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000276584"
SQ   SEQUENCE   1070 AA;  120481 MW;  72856511C907C994 CRC64;
     MLGDGNAGMS TIPGLNQIQF EGFCRFIDQG LTEELYKFPK IEDTDQEIEF QLFVETYQLV
     EPLLKERDAV YESFTYSSEL YVSAGLIWKS RGDMQEQTIF IGNIPLMNSL GTSIVNGIYR
     IVINQILQSP GIYYRSELGH NGISVYTGTI ISDWGGRFEL EIDRKARIWA RVSRKQKVSI
     LVLSAAMGSN LREILENICY PEIFLSFLTN KEKKKIGSKE NAILEFYQQF ACVGGDPVFS
     ESLCKELQKK FFHQRCELGK IGRRNMNQRL NLNIPQNNTF LLPRDVLAAV DHLIGLKFGM
     GTLDDMNHLK NKRIRSVANL LQDQFGLALV RLENVIRGTI CGAIRHKLMP TPQNLVTSTP
     LTTTYDSFFG LHPLSQVLDR TNPLTQIVHG RKLSYLGPGG LTGRTASFRV RDIHPSHYGR
     ICPIDTSEGI NVGLIGSLAI HARIGYWGSL ESPFYEIFEK SKKVRMLYLS PSRDEYYMVA
     AGNSLALNQG SPEEQVVPTR YRQEFLTIAW EQVHLRSIFP FQYFSIGASL IPFIEHNDAN
     RALMSSNMQR QAVPLVRSEK CIVGTGLEPQ VALDSGVPAI AEHEGKIIYT DIDKIVLSGN
     GNTYSIPLIM YQRSNKNTCM HQKPQVGRGK CIKKGQVLAD GAATVGGELA LGKNILVAYM
     PWEGYNFEDA VLISERLIYR DIYTSFHIRK YEIQTHVTSQ GPERITNEIP HLEARLLRNL
     DKNGIVMLGS WVETGDILVG KLTPQAAKES SYAPEDRLLR AILGIQVSTS KETCLKLPIG
     GRGRVIDVRW VQKKGGSSYN PETICVYISQ KREIKVGDKV AGRHGNKGIV SKILPRQDMP
     YLQDGRPVDM VFNPLGVPSR MNVGQIFECS LGLAGGLLNR HYRIAPFDER YEQEASRKLV
     FSELYEASKQ TANPWVFEPE YPGKSRIFDG RTGDPFEEPV LIGKPYILKL IHQVDDKVHG
     RSSGHYALVT QQPLRGRSKQ GGQRVGEMEV WALEGFGVAH ILQEMLTYKS DHIRARQEVL
     GTTIIGETIP NPEDAPESFR LLVRELRSLA LELNHFVVSE KNFQINKKEA