RPOB_CLAM3
ID RPOB_CLAM3 Reviewed; 1162 AA.
AC A5CUC7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CMM_2631;
OS Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382).
OC Bacteria; Actinobacteria; Micrococcales; Microbacteriaceae; Clavibacter.
OX NCBI_TaxID=443906;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCPPB 382;
RX PubMed=18192381; DOI=10.1128/jb.01595-07;
RA Gartemann K.-H., Abt B., Bekel T., Burger A., Engemann J., Fluegel M.,
RA Gaigalat L., Goesmann A., Graefen I., Kalinowski J., Kaup O., Kirchner O.,
RA Krause L., Linke B., McHardy A., Meyer F., Pohle S., Rueckert C.,
RA Schneiker S., Zellermann E.-M., Puehler A., Eichenlaub R., Kaiser O.,
RA Bartels D.;
RT "The genome sequence of the tomato-pathogenic actinomycete Clavibacter
RT michiganensis subsp. michiganensis NCPPB382 reveals a large island involved
RT in pathogenicity.";
RL J. Bacteriol. 190:2138-2149(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AM711867; CAN02714.1; -; Genomic_DNA.
DR RefSeq; WP_012039320.1; NC_009480.1.
DR AlphaFoldDB; A5CUC7; -.
DR SMR; A5CUC7; -.
DR STRING; 443906.CMM_2631; -.
DR PRIDE; A5CUC7; -.
DR EnsemblBacteria; CAN02714; CAN02714; CMM_2631.
DR GeneID; 56886975; -.
DR KEGG; cmi:CMM_2631; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_11; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001564; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1162
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000329170"
SQ SEQUENCE 1162 AA; 128599 MW; F7016A21F2E59548 CRC64;
MAAARNATPT PQNGRDASRL SFAKITDTLT VPDLLALQTE SFDWLVGSDA WKRRVEEGTA
QGRTDLALNS GLEEIFEEIS PIEDLGETMQ LGFTNPYLEE QKYSIDECKE RGKTYSAPLY
VEAEFMNHLT GEIKTQTVFM GDFPLMTEKG TFIINGTERV VVSQLVRSPG VYFERQQEKT
SDKDIYSARV IPSRGAWLEF EIDKRDQVGV RIDRKRKQSV TVFLKALGLT SEQILEEFKG
VASIELTLEK DSILTKEEAL KDIYRKLRPG EQVAAEAARA LLDNFYFNPK RYDLAKVGRY
KINRKLGIDK QLTDSVLTVE DILATIKYLV SLHANETKMN GTRDGKPVEL RLDVDDIDHF
GNRRIRAVGE LIQNQVRTGL SRMERVVRER MTTQDIEAIT PQTLINVRPV VAAIKEFFGT
SQLSQFMDQN NPLAGLTHKR RLSALGPGGL SRERAGVEVR DVHPSHYGRM CPIETPEGPN
IGLIGSLASF ARINSFGFIE TPYRRVVDGV VTDTIDYLTA SEEDEFLVAQ ANAPLTKDFR
FAEDRVLVRP KGGEVELVAK ENVHYMDVSP RQMVSVATSL IPFLEHDDAN RALMGANMQR
QAVPLLRSES PLVGTGMEGY AAIDAGDVLT ADASGVVQEV SAEVVTIQLD EGGTQTYYLR
KFDRSNQGTS YNHRVLVSAG DRIEAGEVIA DGPATENGEL ALGKNLLVAF MPWEGHNFED
AIILSQNLVK DDTLSSIHIE EYEVDARDTK LGKEEITRDL PNVSPELLAD LDERGIIRIG
AEVRPGDILV GKVTPKGETE LSAEERLLRA IFNEKSREVR DTSLKVPHGE QGTIIGVKVF
DSQDGDDELG SGVNQRVVVF IAQKRKITEG DKLAGRHGNK GVISKILPVE DMPFLADGTP
VDVILNPLGI PGRMNFGQVL ETHLGWIAKQ GWEVEGKPKW AERLPDHARQ APAGTKVATP
VFDGALEEEI AGLLDSTTVT RDGDRLIGSS GKTRLFDGRS GEPFPEPVSV GYMYILKLHH
LVDDKIHARS TGPYSMITQQ PLGGKAQFGG QRFGEMEVWA LEAYGAAYAL QELLTIKSDD
ILGRVKVYEA IVKGENIQEP GIPESFKVLI KEMQSLCLNV EVLSADGQAV SLRDTDDEVF
RAAEELGINI STRFESSSID DI
