ID   RPOB_CLOAB              Reviewed;        1241 AA.
AC   Q97EG9;
DT   21-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CA_C3143;
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS   / VKM B-1787).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA   Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE001437; AAK81081.1; -; Genomic_DNA.
DR   PIR; F97286; F97286.
DR   RefSeq; NP_349741.1; NC_003030.1.
DR   RefSeq; WP_010966421.1; NC_003030.1.
DR   AlphaFoldDB; Q97EG9; -.
DR   SMR; Q97EG9; -.
DR   STRING; 272562.CA_C3143; -.
DR   PRIDE; Q97EG9; -.
DR   EnsemblBacteria; AAK81081; AAK81081; CA_C3143.
DR   GeneID; 44999629; -.
DR   KEGG; cac:CA_C3143; -.
DR   PATRIC; fig|272562.8.peg.3324; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1241
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047884"
FT   REGION          1195..1219
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1241 AA;  139249 MW;  DEA50BA38EC4CB78 CRC64;
     MIHPVQVGKR TRMSFSKLDE IGVMPNLIEV QLDSYQWFLD NGLQEIFDDI NPIQDYTGNL
     ILEFIGYKLD MDNIKYSVEE CKERDTTYAA PLKVKVRLLN KETGEVKEQE VFMGDFPLMT
     EQGTFIINGA ERVIVSQLVR SPGAYYDYIV DKNGKKLFSA TVIPNRGAWL EYETDSNSVI
     HVRIDKTRKL PITILVRAMG FGSDAEIVNY FGEEERLKAT IEKDNTKTRE EALLEIYKRL
     RPGEPPTVDS AFSLINSLFF DPKRYDLSRV GRYKFNKKLA VSIRIANKTA AKDIVNPETG
     EIIVEKGQRI SKEKALEIQN SGINSVDISV DDNIIRVIGN NFVDIKSYIK FDISDLNIRE
     LVYYPVLKEI LENYTDEEAI KEQLKKNVHK LVPKHIIKDD MFATVSYELG LAYGIGNVDD
     IDHLGNRRVR SVGELLQNQF RIGLSRMERV VKERMTIQDQ EVITPQALIN IRPVAASIKE
     FFGSSQLSQF MDQINPLSEL THKRRLSALG PGGLSRERAG FEVRDVHHSH YGRMCPIETP
     EGPNIGLINS LATYAKVNEY GFVETPYRTV DKEQGRVTDE IHYITADEED RCLIARANEA
     LDENGYFIDK KITVRAPDEV LVVPANEVDL MDVSARQMVS VATAMIPFLE NDDASRALMG
     SNMQRQAVPL LKPQAPVVGT GIEHKAAVDS GILPKARNAG VVEYVSANEV RVRRDSDGGV
     DTYRLLKFKR SNQGTCINQR PIVEKNEKVE KGTVLADGQS TDLGEIALGR NIRMGFITWE
     GYNYEDAMLI SEQLVRDDVF TSIHIEEYEA EARDTKLGPE EITRDIPNVG EDALKDIDER
     GIIRIGAEVR SGDILVGKVT PKGETELTAE ERLLRAIFGE KAREVRDTSL RVPHGEAGII
     VDVKIFTREN GDELPPGVNE LVRCYIAQKR KISVGDKMSG RHGNKGVISR VLPEEDMPFL
     PDGRPLQICL NPLGVPSRMN IGQVLEVHLG WAAANLGWHI ATPVFDGALE PEIVECLKRA
     GYAEDGKTVL YDGRTGEAFD NRVTVGYMYI LKLAHLVDDK IHARSTGPYS LVTQQPLGGK
     AQFGGQRFGE MEVWALEAYG AAHTLQEILT VKSDDVVGRV KTYEAIVKGE NIPEPGIPEA
     FKVLIKELQA LCLDVKVLSD NNNEIELKES IEDETEDLDV NIEGNEDSKV EPIRPQDVQE
     NVSGENVDAG YENEDVDIDY EDLDIDDLKN GLGLEDFNDE H