ID   RPOB_CLOB6              Reviewed;        1241 AA.
AC   C3KVQ9;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CLJ_B3797;
OS   Clostridium botulinum (strain 657 / Type Ba4).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=515621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=657 / Type Ba4;
RA   Shrivastava S., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A.,
RA   Smith T.J., Sutton G., Brettin T.S.;
RT   "Genome sequence of Clostridium botulinum Ba4 strain 657.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP001083; ACQ53961.1; -; Genomic_DNA.
DR   RefSeq; WP_003360188.1; NC_012658.1.
DR   AlphaFoldDB; C3KVQ9; -.
DR   SMR; C3KVQ9; -.
DR   EnsemblBacteria; ACQ53961; ACQ53961; CLJ_B3797.
DR   KEGG; cbi:CLJ_B3797; -.
DR   HOGENOM; CLU_000524_4_3_9; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000002333; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1241
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000214471"
FT   REGION          1171..1241
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1185..1199
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1200..1241
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1241 AA;  140103 MW;  05828AF158D668CC CRC64;
     MVHPVQVGKR TRMSFSRLKE VGQMPNLIEV QLDSYDWFLK EGLQEVFDDI NPIQDYTGNL
     NLEFVGYKLD LDSIKYSVEE CKERDSTYAA PLKVKVRLLN KETGEIKEQE VFMGDFPLMT
     EQGTFIINGA ERVIVSQLVR SPGVYYDMTV DKTGSKLFSA TVIPNRGAWL EYETDSNNII
     YVRIDKTRKL PITILARALG YGTDAEIIEF FGEDERLKAT IEKDNTKTRE EALLEIYKRL
     RPGEPPTVDS AESLIESLFF DAKRYDLSRV GRYKFNKKLA IHLRITNQIA DQDIVNPQTG
     EILVQKGEKI DKDKAIEIQN CGINEVYIKI DDKSFKVIGN HFVDIHSLIS FDISDLNIKE
     YVFYPVLKEI LDNYADEESI KEEIRKNIYR LIPKHIIRED IYATINYELG LSYDIGYKDD
     IDHLGNRRLR SVGELLQNQF RIGLSRMERV VKERMTIQDQ EVITPQALIN IRPVAASIKE
     FFGSSQLSQF MDQTNPLSEL THKRRLSALG PGGLSRERAG FEVRDVHHSH YGRMCPIETP
     EGPNIGLINS LATFAKVNEY GFIETPYRRI DPKNKRATND IVYMTADEED LYVIARSDEP
     IDENGYFIDD KVTVRAKEEV LVVPVSEVEY MDISPRQLVS VATAMIPFLE NDDASRALMG
     SNMQRQAVPL LKPQAPIVGT GIEYKAATDS GVLPKAKNAG TVAYVSADEI RVRRDSDGGI
     DKYKLLKFKR SNQGTCINQR PIVSKGEVVA KETLLADGPS TDLGEIALGK NILMGFITWE
     GYNYEDAMLI SEQLVREDVF TSIHIEEYEA EARDTKLGPE EITRDIPNVG EEALKDIDER
     GIIRIGAEVR SGDILVGKVT PKGETELTAE ERLLRAIFGE KAREVRDTSL RVPHGEAGII
     VDVKIFTREN GDELPPGVNK LVRCYIAQKR KISVGDKMAG RHGNKGVISR VLPEEDMPFL
     PDGRPLQICL NPLGVPSRMN IGQVLEVHLG LAASKLGWHI ATPVFDGAIE SDIVDCLRKA
     GYSEDGKTVL YDGRTGEPFD NRVTVGYMYI LKLAHLVDDK IHARSTGPYS LVTQQPLGGK
     AQFGGQRFGE MEVWALEAYG AAHTLQEILT VKSDDVVGRV KTYEAIVKGE NIPEPGVPES
     FKVLIKELQA LCLDVKVLND DNQEIKLKES VDEDADELEV NIEGTENQLE DKEEKEEEKE
     KEENYQEDSN EYDDLREEDV EPDLEELSLD DLDLDDFGDE H