ID   RPOB_CLOB8              Reviewed;        1236 AA.
AC   A6LPQ4;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Cbei_0144;
OS   Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium
OS   acetobutylicum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=290402;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51743 / NCIMB 8052;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Sims D., Brettin T., Bruce D., Tapia R., Brainard J., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bennet G.,
RA   Cann I., Chen J.-S., Contreras A.L., Jones D., Kashket E., Mitchell W.,
RA   Stoddard S., Schwarz W., Qureshi N., Young M., Shi Z., Ezeji T., White B.,
RA   Blaschek H., Richardson P.;
RT   "Complete sequence of Clostridium beijerinckii NCIMB 8052.";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000721; ABR32334.1; -; Genomic_DNA.
DR   RefSeq; WP_011967506.1; NC_009617.1.
DR   AlphaFoldDB; A6LPQ4; -.
DR   SMR; A6LPQ4; -.
DR   STRING; 290402.Cbei_0144; -.
DR   PRIDE; A6LPQ4; -.
DR   EnsemblBacteria; ABR32334; ABR32334; Cbei_0144.
DR   KEGG; cbe:Cbei_0144; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000565; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1236
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000086366"
FT   REGION          1193..1212
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1236 AA;  138830 MW;  19DB806EA220B92A CRC64;
     MVHPVQVGKR TRMSFGKVND VTEMPNLIEV QLDSYEWFLR EGLHEVFDDI NPITNFTGNL
     VLEFVDYKLD MENIKYSVEE CKERDATYAA PLKVSIRLQN NETGEIKEQE VFMGDFPLMT
     EQGTFVINGA ERVIVSQLVR SPGVYYNYSI DKSGKKLYSS TVIPNRGAWL EYETDSNDVI
     YVRIDKTRKL SISILARAMG LGSDQELLDF FGEEERFRAS IEKDNTKTKE EALLEIYKRL
     RPGEPPTVDS AISLIDTLFF DAKRYDLSRV GRYKFNKKLA LNLRIANQVA ATDIVNPQTG
     EIMVEKGQKI SRLVAEEIQN AGVKSVDVLI EDKVIRVISN NFVDLKKQVS FDISDLGIKE
     LVHYPTLKEI LDNFSDEASI KEEIKKNISK LIPKHIIRDD IFATISYELG LTYGIGYVDD
     IDHLGNRRLR SVGELLQNQF RIGLSRMERV VKERMTIQDQ EAITPQMLIN IRPVAAAIKE
     FFGSSQLSQF MDQTNPLSEL THKRRLSALG PGGLSRERAG FEVRDVHHSH YGRMCPIETP
     EGPNIGLINS LATFAKVNEY GFIETPYRIV DKENGRATDE IRYFTADEED QCLIAEAKEP
     MDENGHFINK KVRVRHLEDI LVVPTTDVDL IDVSARQIVS VATAMIPFLE NDDASRALMG
     SNMQRQAVPL LSPQAPIVGT GIEFKAAVDS GVLPKAKNAG VVTYVSGSEI RVKRDSDGGT
     DVYKLLKFKR SNSGTCINQR PIVDTGEIVY KNQVIADGPS TDLGEIALGK NIRMGFITWE
     GYNYEDAMLI SEELVREDVF TSMHIEEYEC EARDTKLGPE EITRDIPNVS DDALKDVDDR
     GIIRIGAEVR SGDILVGKVT PKGETELTAE ERLLRAIFGE KAREVRDTSL RVPHGEAGII
     VDIKVFTREN GDELNPGVNE LVRCYIAQKR KISVGDKMAG RHGNKGVISR ILPEEDMPFL
     PDGRPLQICL NPLGVPSRMN IGQVLEVHLG WAASHLGWHI ATPVFDGATQ PEITECMIKA
     GFREDAKTIL YDGRTGEPFD NPVTVGIMYI LKLHHLVDDK IHARSTGPYS LVTQQPLGGK
     AQFGGQRFGE MEVWALEAYG AAHTLQEILT VKSDDVVGRV KTYEAIVKGE NIPEPGVPES
     FKVLIKELQA LCLDIKVLND EHEEVTLKEF VDEDMANLEV NIEGTEEIMV PEPDVLDDDS
     YDQNNDEDID EIDYDESVDI ADLETELELD DFNDEH