RPOB_CLOPS
ID RPOB_CLOPS Reviewed; 1234 AA.
AC Q0SQD6;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CPR_2408;
OS Clostridium perfringens (strain SM101 / Type A).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=289380;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SM101 / Type A;
RX PubMed=16825665; DOI=10.1101/gr.5238106;
RA Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T.,
RA Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H.,
RA Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A.,
RA Khouri H., Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D.,
RA Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J.,
RA Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B.,
RA Paulsen I.T.;
RT "Skewed genomic variability in strains of the toxigenic bacterial pathogen,
RT Clostridium perfringens.";
RL Genome Res. 16:1031-1040(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000312; ABG85828.1; -; Genomic_DNA.
DR AlphaFoldDB; Q0SQD6; -.
DR SMR; Q0SQD6; -.
DR EnsemblBacteria; ABG85828; ABG85828; CPR_2408.
DR KEGG; cpr:CPR_2408; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000001824; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1234
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300302"
SQ SEQUENCE 1234 AA; 138566 MW; 5AC385BAA7BE6F71 CRC64;
MVHPVQVGKR TRMSFAKVKD VAEMPNLIEI QLDSYKWFLD AGLYEVFDDI NPISNFTGNL
VLEFVGYTLD MDNIKYSVEE CKERDTTYAA PLKVAVRLQN KETGEIKEQE VFMGDFPLMT
EQGTFIINGA ERVIVSQLVR SPGVYYNYNV DKTGKKLFSA TVIPNRGAWL EYETDSNDVI
YVRIDKTRKL PISILGRAMG FGSDQELLEY FGEEERFKAT IEKDNTKTKE EALLEIYKRL
RPGEPPTVDS AISLIDSLFF DAKRYDLSRV GRYKFNKKLA IGLRIANQIA AEDIVDKLTG
EVLVAKGEKI SRANAEEIQN RGINSVDVLV EDRVIRIIGN HFVDIHKCVD FDISDLNIRE
LVHYPTLREI LDNYSDEETI KEEIKKNMTR LIPKHIIKDD IFATISYQIG LAYNIGYVDD
IDHLGNRRLR SVGELLQNQF RIGLSRMERV VKERMTIQDQ EAITPQQLIN IRPVAAAIKE
FFGSSQLSQF MDQTNPLSEL THKRRLSALG PGGLSRERAG FEVRDVHHSH YGRMCPIETP
EGPNIGLINS LATYAKVNEY GFIETPYRVV DKSEGRVTGE IRYFTADEED QYLVAQANEP
LDENGCFIDK KVTVRDKGEV LVVPSKDVDL MDVSPRQLVS VATAMIPFLE NDDASRALMG
SNMQRQAVPL LKPYAPIVGT GIEYKAAVDS GVLPKAKNAG EVVYVSANEV RVKRELDGGV
DTYRLLKFKR SNQGTCINQR PIVAKGDWVL KGEVLADGPS TDLGEIALGK NIRMGFITWE
GYNYEDAMLI SEELVREDVF TSIHIEEYEC EARDTKLGPE EITRDIPNVS EDALKDIDER
GIIRIGAEVR SGDILVGKVT PKGETELTAE ERLLRAIFGE KAREVRDTSL RVPHGEAGII
VDVKVFTREN GDDLSPGVNE LVRCYIAQKR KISVGDKMAG RHGNKGVISR VLPEEDMPFL
PDGRPLQICL NPLGVPSRMN IGQVLEVHLG WAASALGWHI ATPVFDGATE TDIEDCLEKA
GYNRNGKTVL RDGRTGEEFD NEVTVGIMYI LKLAHLVDDK IHARSTGPYS LVTQQPLGGK
AQFGGQRFGE MEVWALEAYG AAHTLQEILT VKSDDVVGRV KTYEAIVKGE NIPEPGVPES
FKVLIKELQA LCLDVKVLND NNQEVKFKEL AEDDDEIEVL EVNMEGTEDS TTEEAKEEKG
EAYIPAEEID EEIDYENIDL LDFTSDLDIE DDFN
