ID   RPOB_COFAR              Reviewed;        1066 AA.
AC   A0A327;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Coffea arabica (Arabian coffee).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Rubiaceae; Ixoroideae; Gardenieae complex;
OC   Bertiereae - Coffeeae clade; Coffeeae; Coffea.
OX   NCBI_TaxID=13443;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17309688; DOI=10.1111/j.1467-7652.2007.00245.x;
RA   Samson N., Bausher M.G., Lee S.-B., Jansen R.K., Daniell H.;
RT   "The complete nucleotide sequence of the coffee (Coffea arabica L.)
RT   chloroplast genome: organization and implications for biotechnology and
RT   phylogenetic relationships amongst angiosperms.";
RL   Plant Biotechnol. J. 5:339-353(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; EF044213; ABJ89671.1; -; Genomic_DNA.
DR   RefSeq; YP_817474.1; NC_008535.1.
DR   AlphaFoldDB; A0A327; -.
DR   SMR; A0A327; -.
DR   GeneID; 4421747; -.
DR   Proteomes; UP000515148; Chloroplast Pltd.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..1066
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000276585"
SQ   SEQUENCE   1066 AA;  120328 MW;  F00137D13D95DCAF CRC64;
     MLGDGNEGMA TIPGFNQIQF EGFCRFIDQG LAEELYKFPK IEDTDQEIEF QLFVETYQLV
     EPLIKERDAV YELLTYSSEL YVSAGLIWKT GRDMQEQTIF IGNIPLMNSL GTFIVNGVYR
     ILINQILQSP GIYYRSELDH NGISVYTGTI ISDWGGRSEL EIDRKARIWA RVSRKQKISI
     LVLSSAMGSN LREILENVCY PEIFLSFLND KERKKIGSKE NAILEFYQQF ACVGGDPVFS
     ESLCKELQKK FFQQRCELGR IGRRNMNRRL NLDIPQNNPF LLPRDILAAA DHLIGLKFGM
     GTLDDMNHLK NKRIRSVADL LQDQFGLALV RLENAVRGTI CGAIRHKLIP TPQNLVTSTP
     LTTTYESFFG LHPLSQVLDG TNPLTQIVHG RKLSYLGPGG LTGRTANFRI RDIHPSHYGR
     ICPIDTSEGI NVGLIGSLSI HARIGHWGFL ESPFYEISER RVLFLLPGRD EYYKVAAGNS
     LALNQDIQEK QVVPARYRQE FLTIAWEQVH LRSIFPFQYF SIGASLIPFI EHNDANRALM
     SSNMQRQAVP LSRSEKCIVG TGLEQQAALD SGALSIAERE GKVLYTDTDK ILLSGNGGTL
     SIPLVMYQRS NKNTCMHQKP RVQRGKCIKK GQILADAAAT VGGELSLGKN VLVAYMPWEG
     YNFEDAVLIS ERLVYEDIYT SFHIRKYEIQ THVTSQGPER VTNEIPHLEA HLLRNLDKSG
     IVMLGSWVET GDILVGKLTP QMVKESSYAP EDRLLRAILG IQVSTSKETC LKLPIGGRGR
     VIDVRWIQKK GGSSYNPEMI RVYISQKREI KVGDKVAGRH GNKGIISKIL TRQDMPYLQD
     GRPVDMVFNP LGVPSRMNVG QIFECSLGLA GSLLDRHYRI APFDERYEQE ASRKLVFSEL
     YQASKQTANP WVFEAEYPGK SRIFDGRTGN PFEQPVLIGK PYILKLIHQV DDKIHGRSSG
     HYALVTQQPL RGRAKQGGQR VGEMEVWALE GFGVAHILQE MLTYKSDHIR ARQEVLGTTI
     VGGTIPNPED APESFRLLVR ELRSLALELK HFLISEKNFR IHRKEA