ID   RPOB_COLP3              Reviewed;        1342 AA.
AC   Q47UV9;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CPS_4769;
OS   Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio
OS   psychroerythus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Colwelliaceae; Colwellia.
OX   NCBI_TaxID=167879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=34H / ATCC BAA-681;
RX   PubMed=16043709; DOI=10.1073/pnas.0504766102;
RA   Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X.,
RA   Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M.,
RA   Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A.,
RA   Zhou L., Davidsen T.M., Wu M., Huston A.L., Lewis M., Weaver B.,
RA   Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M.;
RT   "The psychrophilic lifestyle as revealed by the genome sequence of
RT   Colwellia psychrerythraea 34H through genomic and proteomic analyses.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000083; AAZ24907.1; -; Genomic_DNA.
DR   RefSeq; WP_011045494.1; NC_003910.7.
DR   AlphaFoldDB; Q47UV9; -.
DR   SMR; Q47UV9; -.
DR   STRING; 167879.CPS_4769; -.
DR   PRIDE; Q47UV9; -.
DR   EnsemblBacteria; AAZ24907; AAZ24907; CPS_4769.
DR   KEGG; cps:CPS_4769; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000547; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1342
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224046"
SQ   SEQUENCE   1342 AA;  149618 MW;  887B8AEC0663F08C CRC64;
     MAYSYFEKKR IRKDFGKSVQ VMEYPFLLSI QLDSFRKFID TDPTGETGLE AAFRSIFPIK
     AYSGSSELQY VSYRLGEPLF DVKECQIRGV TYSAPLRVKL RLVVYDKEAA AGTVKDIKEQ
     EVYMGEIPLM TDNGTFVING TERVIVSQLH RSPGVFFDHD KGKTHSSGKV LYNARVIPYR
     GSWLDFEFDP KDNLFVRIDR RRKLPASIIL RALEYSTEEI LAMFYDTTDY TIKGDKLIMD
     LIPERLRGET AIFDISIKKG EVLVESGRRI TARHIRALSK AKLEKLEVPA DYIVGRVLSK
     AYIDKSTGEV IAEANAIITL ELLAELSQAG HKVLSTLYMN EFDVGSYMSD TLRVDSSTNK
     LEALVEVYRM MRPGEPPTKD AAEGLFSNLF FASERYDLST VGRMKFNRRV GNADDVGTGI
     LSKEDIISVM KTLIGIRDGK GEVDDIDHLG NRRIRSVGEM AENQFRVGLV RVERAVRERL
     SLGDLDAIMP QDLINAKPIS AAVKEFFGSS QLSQFMDQNN PLSEVTHKRR ISALGPGGLT
     RERAGFEVRD VHPTHYGRVC PIETPEGPNI GLINSLSCYA RTNDFGFLET PYRKVIDGLV
     TDEIDYLSAI EEGNFVIAQA NAERNDSNKL VQDLVNCRHR NEFILKASAE VQYMDVSPQQ
     IISVAASLIP FLEHDDANRA LMGSNMQRQA VPTLRVDKPL VGTGMEKVVA VDSGVTAVAK
     RGGVVSYVDA SRIVVKVNEN EMHAGEAGID IYNLTKYTRS NQNTCINQRP VCRMGEPVVR
     GDVLADGPST DMGELALGQN MRIAFMPWNG YNFEDSMLLS ERVAIEDRFT TIHIQELTCI
     ARDTKLGSEE ITADIPNVGE SALSKLDEAG VVYIGAEVNG GDILVGKVTP KGETQLTPEE
     KLLRAIFGEK AADVKDSSLR VPNSVKGTII DVQIFTRDGV EKDARAVEIE QMQLKEVKKD
     LGDELSILED GIYARTKKLL LSAGLNESDL TSMSRDKWLT QNLADEGQQA ELEQIAEQFD
     NIKEDFDKKF EVKRRKITQG DDLQPGVLKI VKVYLAVKRH IQPGDKMAGR HGNKGVISNV
     VPVEDMPYDQ FGVPVDIVLN PLGVPSRMNI GQILETHLGM ACRGIGEKIN RMLEAQQEIH
     KLRNFIQEVY NVGESRQVVD VASFSDDEVL RLAGNLRAGL PIATPAFDGA AEKEIKELFV
     LADMPQSGQF VLTDGRTGRE FERPVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG
     GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVNG RTKMYKNLVD GDHRMEPGIP
     ESFNVLLKEI RSLGINIELD KD