RPOB_CORDI
ID RPOB_CORDI Reviewed; 1175 AA.
AC P60280;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=DIP0446;
OS Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype
OS gravis).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=257309;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700971 / NCTC 13129 / Biotype gravis;
RX PubMed=14602910; DOI=10.1093/nar/gkg874;
RA Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G.,
RA Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D.,
RA De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N.,
RA Holroyd S., Jagels K., Moule S., Quail M.A., Rabbinowitsch E.,
RA Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G.,
RA Parkhill J.;
RT "The complete genome sequence and analysis of Corynebacterium diphtheriae
RT NCTC13129.";
RL Nucleic Acids Res. 31:6516-6523(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; BX248355; CAE48951.1; -; Genomic_DNA.
DR AlphaFoldDB; P60280; -.
DR SMR; P60280; -.
DR STRING; 257309.DIP0446; -.
DR PRIDE; P60280; -.
DR EnsemblBacteria; CAE48951; CAE48951; DIP0446.
DR KEGG; cdi:DIP0446; -.
DR HOGENOM; CLU_000524_4_3_11; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000002198; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1175
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047887"
FT REGION 1142..1175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1175 AA; 129791 MW; 044D703793C357DB CRC64;
MVNIAGPPPR EVLEGPILAV SRQTKANIPG APERKSFAKI TEPIEVPGLL DIQLNSFAWL
IGTPEWRARQ QEELGDSVRV TSGLEDILEE LSPIQDYSGN MSLSLSEPRF EDMKNTIDEC
KDKDINYSAP LYVTAEFINN ETQEIKSQTV FIGDFPMMTD KGTFIVNGTE RVVVSQLVRS
PGVYFDQTID KSTERPLHSV KVIPSRGAWL EFDVDKRDTV GVRIDRKRRQ PVTVLLKALG
WTTEQITERF GFSEIMMSTL ESDGVSNTDE ALLEIYRKQR PGEQPTRDLA QSLLDNSFFR
AKRYDLAKVG RYKVNRKLGL GGDNEGLMTL TEEDIATTLE YLVRLHAGET TMTSPTGEVI
PVETDDIDHF GNRRLRTVGE LIQNQVRVGL SRMERVVRER MTTQDAESIT PTSLINVRPV
SAAIREFFGT SQLSQFMDQN NSLSGLTHKR RLSALGPGGL SRERAGIEVR DVHASHYGRM
CPIETPEGPN IGLIGSLASY ARVNAFGFIE TPYRKVENGV LTDQIDYLTA DEEDRFVVAQ
ANVEHDADGK ITADSVTVRV KNGDIQVVAP ESVDYLDVSP RQMVSVATAM IPFLEHDDAN
RALMGANMQR QAVPLVRSEA PFVGTGMERA AAYDAGDLII NKKGGVVENV SADIITVMAD
DGTRETYILR KFERTNQGTC YNQTPLVNIG DRVEAGQVLA DGPGTHNGEM SLGRNLLVAF
MPWEGHNYED AIILNQRVVE EDILTSIHIE EHEIDARDTK LGPEEITREI PNVSEDVLKD
LDERGIVRIG ADVRDGDILV GKVTPKGETE LTPEERLLRA IFGEKAREVR DTSMKVPHGE
TGKVIGVRRF SRDDDDDLAP GVNEMIRVYV AQKRKIQDGD KLAGRHGNKG VVGKILPQED
MPFMPDGTPV DIILNTHGVP RRMNIGQVLE VHLGWLAAAG WKIDTEDPAN AELLKTLPED
LYDVPAGSLT ATPVFDGATN EEIAGLLGNS RPNRDGDVMV DENGKATLFD GRSGEPFPYP
VSVGYMYILK LHHLVDEKIH ARSTGPYSMI TQQPLGGKAQ FGGQRFGEME VWAMQAYGAA
YTLQELLTIK SDDVVGRVKV YEAIVKGENI PDPGIPESFK VLLKELQSLC LNVEVLSADG
TPMELSGSDD DEFDQAGASL GINLSRDERS DADIA
