ID   RPOB_COXB2              Reviewed;        1375 AA.
AC   B6J270; O87903;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 2.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CbuG_1774;
OS   Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae;
OC   Coxiella.
OX   NCBI_TaxID=434923;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9511749; DOI=10.1016/s0378-1119(97)00618-5;
RA   Mollet C., Drancourt M., Raoult D.;
RT   "Determination of Coxiella burnetii rpoB sequence and its use for
RT   phylogenetic analysis.";
RL   Gene 207:97-103(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CbuG_Q212;
RX   PubMed=19047403; DOI=10.1128/iai.01141-08;
RA   Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D.,
RA   Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E.,
RA   Heinzen R.A.;
RT   "Comparative genomics reveal extensive transposon-mediated genomic
RT   plasticity and diversity among potential effector proteins within the genus
RT   Coxiella.";
RL   Infect. Immun. 77:642-656(2009).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ACJ19048.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U86688; AAC61666.1; -; Genomic_DNA.
DR   EMBL; CP001019; ACJ19048.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; B6J270; -.
DR   SMR; B6J270; -.
DR   KEGG; cbg:CbuG_1774; -.
DR   HOGENOM; CLU_000524_4_0_6; -.
DR   OMA; FMTWEGY; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1375
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000366031"
SQ   SEQUENCE   1375 AA;  153672 MW;  EA2821A144699297 CRC64;
     MAQANSRSRN SATISYPEKR RARVNLGKRE VEILKTPYLL ETQIESYRKF LQKDIAAEKR
     EDNGLHAAFK SVFPITSYSG YAVLEYVGYS LGGESTLFDV EECKLRGLTY AAPLKVNMRL
     VIYDKEAPAG KKAIKDIKEQ EVYMGEIPLM TETGSLVING TERVVVSQLH RSPGVFFEHD
     KGKTHSSGKL LYSARVIPYR GSWLDFEFDP KDCLFVRIDR RRKLPATVIL RALGYDTEQI
     LDMFYKTNHF HLNQETVTLD LIPQRLRGEL AVVEIKDKKG KVIVEANRRI SARHIRLIEK
     AEINKLELPD DYLYGKVIGK TIIDKETGEI IAHANEEITA ELLKNLRATK TASLDTLYIN
     EIECGPYVSD TLRLDTTTNQ LEALVEIYRI MRPGEPPTKE AAESLFENLF FSPERYSLSA
     VGRMKFNRRV GRKELTGLDV LSKEDIVDVL RVLVDIRDGK GDVDDIDHLG NRRIRSVGEM
     AENQFRVGLV RVERAVKDRL SLADVENLMP QDLVNAKPVS AAIKEFFGSS QLSQFMDQNN
     PLSEITHKRR VSALGPGGLT RERAGFEVRD VHVTHYGRVC PIETPEGPNI GLINSLAVFA
     RANEYGFLET PYRKVVDRVV TDETEYLSAI EEGDYYIAQA NTNVDEKGRL VDDLISCRYK
     GEFTLTTPDK INYMDVSPRQ IVSVAAALIP FLEHDDANRA LMGSNMQRQA VPTIRPETPL
     VGTGMERTVA VDSGVTVIAK RSGVIDSVDA SRIVVRVDRK ETEDDDDIGV DIYNLTKFTR
     SNQNTCINQH PIVEVGDKVQ KGDVLADGPS TDIGELALGQ NLLVAFMPWN GYNFEDSILI
     SERLVEEDRF TTIHIQEFTC VARDTKLGPE EITSDIPNVG ESALAKLDES GIVHIGAEVN
     AGDILVGKVT PKGETQLTPE EKLLRAIFGE KASDVKDTSL RVTPGITGTV IDVRIFTREG
     VKKDERTLEI EKAELSKVEK DLNDELRVRE DALFENLEKL LTGRVAAGGP NKLAKGTKIT
     KSYLADLPRQ KWFEIRLQDD AATKRLEASH EHFKELRETR DAKLKDSRQK LTQGGDLAPG
     VIKIVKVYLA VKRRIQPGDK MAGRHGNKGV ISTIVPIEDM PYLEDGTPVD IVLNPLGVPS
     RMNIGQVLET HLGWAAKGLG KKIGEMIEKG ADAKELRKSL KPIYDLSKTQ RFDLEALEDP
     EIVMLAKNLR KGVPISSPVF DGATEEEIKQ LLKMADLPTS GQAALYDGRT GKKFDRSVTV
     GYMYMLKLNH LVDDKMHARS TGSYSLVTQQ PLGGKAQFGG QRFGEMEVWA LEAYGAAYTL
     QEMLTVKSDD VAGRTRMYKN IVDGDHRMDA GMPESFNVLV KEIRSLAIDI GLEND