RPOB_CUPMC
ID RPOB_CUPMC Reviewed; 1368 AA.
AC Q1LI20;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Rmet_3334;
OS Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS CH34) (Ralstonia metallidurans).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=266264;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT master survivalist in harsh and anthropogenic environments.";
RL PLoS ONE 5:E10433-E10433(2010).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000352; ABF10206.1; -; Genomic_DNA.
DR RefSeq; WP_008649192.1; NC_007973.1.
DR AlphaFoldDB; Q1LI20; -.
DR SMR; Q1LI20; -.
DR STRING; 266264.Rmet_3334; -.
DR PRIDE; Q1LI20; -.
DR EnsemblBacteria; ABF10206; ABF10206; Rmet_3334.
DR GeneID; 60826583; -.
DR KEGG; rme:Rmet_3334; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000002429; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1368
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300381"
SQ SEQUENCE 1368 AA; 152496 MW; 7726E8CFF266CA4D CRC64;
MAYSFTEKKR IRKSFAKRAT VHQVPFLLAT QIESYTQFLQ AETPPARRKS EGLQAAFNAI
FPISSHNGLA RMEFVSYHLS NPPFDVKECQ QRGLTFHSAL RAKVRLIIND RENPGKVKEV
KEQEVYMGEI PLMTSTGSFV INGTERVIVS QLHRSPGVFF EHDKGKTHSS GKLLFSARII
PYRGSWLDFE FDPKDILYFR VDRRRKMPVT ILLKSIGLTP EQILAHFFVF DNFTLQSEGA
QLEFVPERLR GEVARFDIVD KNGRVVVEKD KRINAKHIRD LDSAGTKLIS VPEDYLLGRV
LAKNIIDPDT GEVIANANDE LTETLLENLR EAGVKDIQTL YTNDLDQGPY ISQTLRADDT
ADQTAARIAI YRMMRPGEPP TEDAVEALFQ RLFYSEESYD LSRVGRMKVN SRLSRPSGEG
SMVLQDEDIL ETIKILVNLR NGKGEVDDID HLGNRRVRCV GELAENQFRA GLSRVERAVK
ERLGQAETEN LMPHDLINSK PISSAIREFF GSSQLSQFMD QTNPLSEITH KRRVSALGPG
GLTRERAGFE VRDVHPTHYG RVCPIETPEG PNIGLINSLA LYARLNEYGF LETPYRKVEN
SKLTDQVDYL SAIEEGKYVV AQANATVDAE GNLIDELVSA REGSERETRM VTPDRVQYID
VAPSQIVSAA ASLVPFLEHD DANRALMGAN MQRQAVPCLR PDKPLVGTGI ERTVAVDSGT
AVQATRGGVV DYVDAMRVVI RVNDDEAVAG EVGVDIYNLI KYTRSNQNTN INQRPMVKVG
DIVARGDVIA DGASTDLGEL ALGQNMLVAF MPWNGYNFED SILISERVVA EDRYTSIHIE
ELSVVARDTK LGPEEITRDI SNLAEAQLAR LDESGITYIG AEVEAGDVLV GKVTPKGETQ
LTPEEKLLRA IFGEKASDVK DTSLRVPSGM SGIVIDVQVF TREGVTRDKR AQSIIDDELK
RYRLDLNDQL RIVEGDAFQR LERMLLDKTV NGGPKKLAKG AKLTREYLAD LDKYHWFDIR
PADEEVAAQL EAVKEAIEQK RHEFDLAFEE KRKKLTQGDE LPPGVIKMVK VYLAVKRRLQ
PGDKMAGRHG NKGVVSKIVP IEDMPYMADG TPADIVLNPL GVPSRMNVGQ ILETHLGWAA
RGLGQRIGDM LKASAKAQEL RPLLAQIYNE SGKAEDLDSL SDAEVLELAG NLKKGVPFAT
PVFDGAHEAE IRRMLDLAYP DDIAKERGLT ASKQQVTLHD GRTGEAFERP VTLGVMHMLK
LHHLVDDKMH ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAS YVLQEMLTVK
SDDVNGRTKV YENIVKGEHS IDAGMPESFN VLVKEIRSLG IDIDLDRY
