RPOB_CUSRE
ID RPOB_CUSRE Reviewed; 1070 AA.
AC A7M958;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=DNA-directed RNA polymerase subunit beta;
DE EC=2.7.7.6;
DE AltName: Full=PEP;
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta;
DE Short=RNA polymerase subunit beta;
GN Name=rpoB;
OS Cuscuta reflexa (Southern Asian dodder).
OG Plastid.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Convolvulaceae; Cuscuteae; Cuscuta;
OC Cuscuta subgen. Monogynella.
OX NCBI_TaxID=4129;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND RNA EDITING.
RX PubMed=17714582; DOI=10.1186/1471-2229-7-45;
RA Funk H.T., Berg S., Krupinska K., Maier U.-G., Krause K.;
RT "Complete DNA sequences of the plastid genomes of two parasitic flowering
RT plant species, Cuscuta reflexa and Cuscuta gronovii.";
RL BMC Plant Biol. 7:45-45(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6;
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid.
CC -!- RNA EDITING: Modified_positions=113 {ECO:0000269|PubMed:17714582}, 158
CC {ECO:0000269|PubMed:17714582}, 184 {ECO:0000269|PubMed:17714582}, 189
CC {ECO:0000269|PubMed:17714582}, 667 {ECO:0000269|PubMed:17714582};
CC Note=Editing at positions 113 and 158 is more efficient in
CC photosynthetically active tissue.;
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000305}.
CC -!- CAUTION: Young tissue from this organism is photosynthetic and contains
CC some thylakoids, although the photosynthetic activity does not exceed
CC the light compensation point. {ECO:0000305}.
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DR EMBL; AM711640; CAM98386.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; YP_001430100.1; NC_009766.1.
DR AlphaFoldDB; A7M958; -.
DR SMR; A7M958; -.
DR GeneID; 5536671; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 2: Evidence at transcript level;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid; RNA editing;
KW Transcription; Transferase.
FT CHAIN 1..1070
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000308474"
SQ SEQUENCE 1070 AA; 120490 MW; 38BB02FAA4C9D643 CRC64;
MLVDGKGGIT TIPGLNQIQL EGFCRFIDQG LMEELSKFQK IEDIDQEIEF QLFVETYQLV
EPLIKERDAV YDSLTYSSEL YVSAGLIRKA SKDMQEQTIF IGSLPIMNSL GTFIVNGIYR
IVINQILQSP GIYYRSELDQ NGISVYTGTI ISDWGGRLEL EIDRKARVWA RVSRKQKISI
LVLLSAMGLN LREILENVCY PEILLSFLRD KEKKKIGSKE NAILEFYKKF ACVGGDPLFS
ESLCKELQNK FFQQRCELGR IGRRNMNRRL HLDIPHNNTF LLPRDILEAT DHLIGLKFGM
GTLDDMNHLQ NKRIRSVADL LQDQFGLALV RLENAVQGTL CGAIRHKRIP TPQNLVTSTL
LTTTYESFFG LHPLSQVLDG TNPLTQIVHA RKVSSLGPGG LTGRTASFRI RDIHPSHYGR
ICPIDTSEGI NVGLIGSLAI HVRIGNWGSL ESPFYEISDR LTGVRVLHLS PGRDEYYMVA
AGNSLALNQD IQEDQVVPAR YRQEFLTIAW EQVNLRSIFP FQYFSIGASL IPFIEHNDAN
RALMSSNMQR QAVPLTWSEK CIVGTGMERQ AALDSGSLAI AEREGRVIYT DTEKILVSGD
GKTINIPLVM YQRSNKNTCM YQQPQVRRGQ FIKKGQILAG GAATVEGELA LGKSVLVAYM
PWEGYNFEDA VLISECLVYE DIFTSFHIKK YEIQIHMTTQ GPEKVTNEIP HLEAHLIRNL
DKNGIVLQGS WVEPGDVLVG KLTPQVVKES AYAPEDRLLR AILGIPVSAS KETCLKVPIG
ARGRVIDVRW IQKKGGYGYN PEKIRVYILQ KREIKVGDKV AGRHGNKGII SKILPRQDMP
YLQDGRSVDL VFNPLGVPSR MNVGQIFECS LGLAGSLLDR HYRIAPFDER YEQEASRKIV
FSELYEASKQ TANPWAFEPE YPGKSRIFDG RTGKTFEHPV LIGKPYILKL IHQVDDKIHG
RSIGHYALVT QQPLRGRAKQ GGQRVGEMEV WALEGFGVAH ILQEMLTYKS DHIRARQEVL
GTTIVGGTIP SPKNAPESFR LLVRELRSLA LELTHFLVSE KNFQVNRKEA
