RPOB_CYAPA
ID RPOB_CYAPA Reviewed; 1103 AA.
AC P48119;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Cyanophora paradoxa.
OG Plastid; Cyanelle.
OC Eukaryota; Glaucocystophyceae; Cyanophoraceae; Cyanophora.
OX NCBI_TaxID=2762;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX LB 555 / Pringsheim;
RA Stirewalt V.L., Michalowski C.B., Loeffelhardt W., Bohnert H.J.,
RA Bryant D.A.;
RT "Nucleotide sequence of the cyanelle DNA from Cyanophora paradoxa.";
RL Plant Mol. Biol. Rep. 13:327-332(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX LB 555 / Pringsheim;
RA Loeffelhardt W., Stirewalt V.L., Michalowski C.B., Annarella M.,
RA Farley J.Y., Schluchter W.M., Chung S., Newmann-Spallart C., Steiner J.M.,
RA Jakowitsch J., Bohnert H.J., Bryant D.A.;
RT "The complete sequence of the cyanelle genome of Cyanophora paradoxa: the
RT genetic complexity of a primitive plastid.";
RL (In) Schenk H.E.A., Herrmann R., Jeon K.W., Mueller N.E., Schwemmler W.
RL (eds.);
RL Eukaryotism and symbiosis, pp.40-48, Springer-Verlag, Heidelberg (1997).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, cyanelle.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; U30821; AAA81261.1; -; Genomic_DNA.
DR PIR; T06918; T06918.
DR RefSeq; NP_043230.1; NC_001675.1.
DR AlphaFoldDB; P48119; -.
DR SMR; P48119; -.
DR GeneID; 801641; -.
DR GO; GO:0009842; C:cyanelle; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Cyanelle; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1103
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000048021"
SQ SEQUENCE 1103 AA; 125355 MW; 120CD4FBF0B41744 CRC64;
MTQLTDTSLI PFDLLEIQKA SFKWFFEEGL IEEIENFSPV IDLRGNFEVH FYLKNYELEA
PTFTLEEAKQ RDLTYAAQLY IPVELRDLET GIVKQDRIFF GEIPLMTDRC TFLINGVERV
IINQIIRSPG IYYSVNTDEQ GTRTYDVTII SNRGAWLKFE VDKDDLIWMR IDKTHRLPCH
IFLKALNLSE SEILSALTHP EFLQKTIYEH GDCTEEEALI EFHRQLRPGE PPNSIQGRYL
LYSRFFDPKR YDLGFVGRYK LNKKLNLNVS PNIRILTTQD ILEILNYLIN LQFGMGQIDD
IDHLGNRRVK AVGELLQSQL RIGLNRLERL IHDRLSMLTT KSFKKRKKLT TLINAKPINE
CLKEFFGSSQ LSQFMDQTNP LAELIHKRRL TILGPGGLSR DRAGCAVRDI HPSHYGRICP
IETPEGQNAG LVGSLTTHAH LNQYGFIETP FYKVEKGYIQ KELGIIYLTA DEEDQYRIAA
ADVLINETNK IIGEDISVRY RQEFITTKID QIDFIAISPL QTFSVSTSLI PFLEHDDANR
ALMGSNMQRQ AVPLLHAEKP LVGTGLEFQV AKDSRRVLIN KSEGLVKRVT GDHICIQTDT
GKEINYTLIK YQRSNQDTCI TQRPIVFEGE RVKKGQILAD DTATDKGELA LGQNLLVAYM
PWEGYNYEDA ILINERLVYE DVYTSIHIEK YETETRQTKL GIEEITREIP NVNEYSLRNL
DEKGIVSVGS WIKGGDILVG KVAPKAESDQ PPEGKLLKAI FGEKNRDVRD TSLRMPSGEK
GRIVDVRIFI REFGEMLYPG ANTANTIVRI YIAQKRKIQV GDKMAGRHGN KGIISKILPR
QDMPYLPDGT PIDIILNPLG VPSRMNVGQV YECLLGWAAE HLGVRFKLIP FDERFGKQAS
RTFIHEKLKE AKELTNKNWL FNSNHPGKIQ LFDGRTGESF DNPIMVGKAY MLKLVHLVDD
KIHARSTGPY SLITQQPLGG KAQQGGQRFG EMEVWALEAF GAAYTLQEIL TIKSDDMLGR
NEALKAIVKG KAIPRPGIPE SFKVLMRELQ ALCLDVVAYK IQNGDQDECE ALAIDLSGSL
ATPIDNIIKE VIQQEELGEE LTS
