RPOB_CYTH3
ID RPOB_CYTH3 Reviewed; 1291 AA.
AC Q11QA5;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CHU_3169;
OS Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC
OS 15051 / NCIMB 9469 / D465).
OC Bacteria; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae;
OC Cytophaga.
OX NCBI_TaxID=269798;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465;
RX PubMed=17400776; DOI=10.1128/aem.00225-07;
RA Xie G., Bruce D.C., Challacombe J.F., Chertkov O., Detter J.C., Gilna P.,
RA Han C.S., Lucas S., Misra M., Myers G.L., Richardson P., Tapia R.,
RA Thayer N., Thompson L.S., Brettin T.S., Henrissat B., Wilson D.B.,
RA McBride M.J.;
RT "Genome sequence of the cellulolytic gliding bacterium Cytophaga
RT hutchinsonii.";
RL Appl. Environ. Microbiol. 73:3536-3546(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000383; ABG60409.1; -; Genomic_DNA.
DR RefSeq; WP_011586518.1; NZ_FPJX01000011.1.
DR AlphaFoldDB; Q11QA5; -.
DR SMR; Q11QA5; -.
DR STRING; 269798.CHU_3169; -.
DR EnsemblBacteria; ABG60409; ABG60409; CHU_3169.
DR KEGG; chu:CHU_3169; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_10; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001822; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1291
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300305"
SQ SEQUENCE 1291 AA; 145290 MW; 34340FF8E004FE51 CRC64;
MATNNTQNLR KSFAKTRSTV EYPDFLDIQV RSFKDFFQID TPAENRFKEG LYKVFAENFP
ISDSRDNFIL DFIDYNIDTP KYNVDECIDR GLTYAVPLKA KLRLTCNDKD NEDFQTIEQE
VFLGNIPYMT ERGSFVINGA ERVIVSQLHR SPGVFFAQSK HTNGTKLYSA RVIPFKGSWI
EFATDVNNVM FAYIDRKKKF PVTTLLRAIG YGSDKDILDL FGLSEEVEAT KANIKKAIGR
KLAARVLRTW TEDFVDEDTG EVVSIDRNEV LLERDSIIKE EDINIIVESG SKSIILHRED
VNIADYTIIY NTLQKDNSNS EKEAVEQIYR QLRNTDAPDE QTARDIIHNL FFSDKRYDLG
EVGRYRINKK LGSGMGSEVK VLTKEDIINI VKYLIGLINS RAVVDDIDHL SNRRVRTMGE
QLYSQFSVGL ARMARTIKER MNVRDNEDFK PVDLINARTL SSVINSFFGT NQLSQFMDQT
NPLAEITHKR RMSALGPGGL SRERAGFEVR DVHYTHYGRL CTIETPEGPN IGLISSLCVH
AKVNSMGFIE TPYRKVIDGI VDVKNQPVFL TAEEEDGAHI AQANAMYDDN GNFISDRIKA
RYEGDFPVIA PDKVDLIDIA TNQIVSVAAS LIPFLEHDDA NRALMGSNMQ RQAVPLLRPE
APIVGTGLER RVAMDSRTLL IAEADGVVEF VDANEIKMRY DLTDEDRLVS FDSDIITYSL
IKFRRTNQDT CINLKPVIQH GERVKKGQVL CEGYATDKGE LALGRNLMVA FMPWQGYNFE
DAIVISEKVV RDDIFTSIHI EEFELEVRDT KRGEEELTSE IPNVSEEAVK NLDENGIIRT
GAEIKEGDIL IGKITPKGES DPTPEEKLLR AIFGDKAGDV KDASMKAPPS LRGVVIDTKL
FSRPKKDKDL RIKSKKQVET IKGKYSKDLL ALREQMIEKL AALLDGQTSQ GVKHKFGDEV
ISKGVKFSRK NIEANVFPDK NPYRDESNYN VQEEANLLGD LIIDNWTSDK KINENVLRLV
KNYLTKRNET AGKFKRERFT LEVGDELPAG IVQLAKVYIA KKRKLKVGDK MAGRHGNKGV
VARIVRDEDM PFLEDGTPVD IVLNPLGVPS RMNIGQIYET LLGLAGKKLG RKYATPIFDG
ASEEEVINEL RDAGLPTIGR TKLVDGLSGN PFDQPVTVGV IYMMKLGHLV DDKMHARSIG
PYSLITQQPL GGKAQFGGQR FGEMEVWALE AFGAANILQE ILTVKSDDVV GRAKTYECIV
KGENLPRPNI PESFNVLVHE LRGLALEITL H
