ID   RPOB_DAUCA              Reviewed;        1070 AA.
AC   Q0G9X0;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Daucus carota (Wild carrot).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Danvers Half-long;
RX   PubMed=16945140; DOI=10.1186/1471-2164-7-222;
RA   Ruhlman T., Lee S.-B., Jansen R.K., Hostetler J.B., Tallon L.J., Town C.D.,
RA   Daniell H.;
RT   "Complete plastid genome sequence of Daucus carota: implications for
RT   biotechnology and phylogeny of angiosperms.";
RL   BMC Genomics 7:222-222(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; DQ898156; ABI32416.1; -; Genomic_DNA.
DR   RefSeq; YP_740109.1; NC_008325.1.
DR   AlphaFoldDB; Q0G9X0; -.
DR   SMR; Q0G9X0; -.
DR   GeneID; 4266719; -.
DR   OrthoDB; 944344at2759; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1070
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000276586"
SQ   SEQUENCE   1070 AA;  120810 MW;  8083976FBD1DDB95 CRC64;
     MLRDGNEGMS TIPGFNQIQF EGFCRFMDQG LTEELYKFPK IEDTDQEIEF QLFVETYQLV
     EPLIKERDAV YESLTYSSEF YISAGLIWKT SRDMQEQTIF LGNIPLMNSL GTSIVNGIYR
     IVINQILQSP GIYYRSELDH NGISVYTGTI ISDWGGRLEL EIDKKARIWA RVSRKQKISI
     LVLSSAMGSN LREILENVCY PEIFLSFLTD KEKKKIGSKE NAILEFYQQF ACVGGDPVFS
     ESLCKELQKK FFQQKCELGK IGRRNMNRRL RLDISQNNTF LLPRDILAAA DHLIGMKFGM
     GTLDDMNHLK NKRIRSVADL IQDQLGLALV RLENMIRGTI GGALRHKLIP SPQNLVTSTP
     LTSTYESFFG LHPLSQVLDR TNPLTQIVHG RKLSYLGPGG LTGRTASFRI RDIHPSHYGR
     ICPIDTSEGI NVGLIGSLAI HAKIGRGGSL ESPFYEISQR SKGARMLYLS PGKDEYYMVA
     AGNPLALNQG LQEEQVVPAR YRQEFLTIAW EQVHLRSIFS FQYFSIGASL IPFIEHNDAN
     RALMSSNMQR QAVPLSRSEK CIVGTGLERQ AALDSGVLAI AEHEGKVIYT DTDKILLSGN
     GDTLNIPLVM YQRSNKNTCM HQKPQVQRGK YIKKGQILAY GAATIGGELA LGKNVLVAYM
     PWEGYNFEDA VLISERLVYE DIYTSFHIRK YEIQTHVTSQ GPERVTREIP HLEAHLLRNL
     DKNGIVMLGS WVETGEILVG KLTPQMVKES SYAPEDRLLR AILGIQVSTS KETCLKLPIG
     GRGRVIDVRW IQKRVGSSYN PETIRVYILQ KREIKVGDKV AGRHGNKGII SKILPRQDMP
     YLQDGRPVDM VFNPLGVPSR MNVGQIFECS LGLAGGLLDR HYRIAPFDER YEQEASRKLV
     FSELYQASKQ TATPWVFEPE YPGKSRIFDG RTGDPFEQPV IIGKPYILKL IHQVDDKIHG
     RSSGHYALVT QQPLRGRAKQ GGQRVGEMEV WALEGFGVAY ILQEMLTYKS DHIRARQEVL
     GTTIIGGAIP NPEDAPESFR LLVRELRSLA LELNHFFVSE KTFKIKRKEA