RPOB_DECAR
ID RPOB_DECAR Reviewed; 1427 AA.
AC Q47JB0;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Daro_0312;
OS Dechloromonas aromatica (strain RCB).
OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Azonexaceae;
OC Dechloromonas.
OX NCBI_TaxID=159087;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RCB;
RX PubMed=19650930; DOI=10.1186/1471-2164-10-351;
RA Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G.,
RA Lapidus A.;
RT "Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB:
RT indications of a surprisingly complex life-style and cryptic anaerobic
RT pathways for aromatic degradation.";
RL BMC Genomics 10:351-351(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000089; AAZ45071.1; -; Genomic_DNA.
DR RefSeq; WP_011286081.1; NC_007298.1.
DR AlphaFoldDB; Q47JB0; -.
DR SMR; Q47JB0; -.
DR STRING; 159087.Daro_0312; -.
DR EnsemblBacteria; AAZ45071; AAZ45071; Daro_0312.
DR KEGG; dar:Daro_0312; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 3.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1427
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000224048"
SQ SEQUENCE 1427 AA; 158061 MW; 5844AF58C08EAB6B CRC64;
MTYSFTEKKR IRKSFAKRAS VLDVPYLLAT QLQSFKDFLQ DEVAPEKRKN EGLQAAFTSI
FPIVSHSGNA RLEFVSYMLG EPAFDVTECQ QRGLTFASSL RARVRLVIMD REAPDTVKEV
KEQEVYMGEI PLMTTNGSFV INGTERVIVS QLHRSPGVFF EHDRGKTHSS GKLLFSARVI
PYRGSWLDFE FDPKDTLFFR VDRRRKMPVT TLLKAIGMSS EEILDQFFDF DTFLLSKDKV
EFTLVPERLR GEVARFDFVG PDGVLIVPKD KRITAKHIRD IGAAAIKQIV VPEDFLIGRV
VAKNIVDKAT GEVVANANDE ITETLLAKLR EAEITTVETL YTNELDRGAF ISNTLRADET
ATRQAARVAI YRMMRPGEPP TEEAVEILFN GLFYSDERYD LSGVGRMKFN RRLARQDVIE
YKLMVKGLAS KAEAALKNLA EASGFALSAI QDLVSLMPFG ARAMVENSTL SEVEALAAKL
KPLGANVEVR EQLTLSPRDI VEVIKILVEL RNGRGEIDDI DHLGNRRVRS VGELAENQFR
AGLVRVERAV KERLSQAESD NLMPHDLINA KPISAAIKEF FGSSQLSQFM DQTNPLSEIT
HKRRVSALGP GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL ALFAQVNSYG
FIETAYRKVN NGQVTSDIEY LSAIEEGNYV VAQANASLDA NGRLSDDLVT CREKGETILA
EPGRVQYMDV APGQIVSVAA SLIPFLEHDD ANRALMGANM QRQAVPCLRP EKPLVGTGIE
RTVAVDSGTA VVALRGGMVD YVDAARVVVR VNDDETIAGE VGVDIYNLVK YTRSNQNTNI
NQRPMVRVGD HIAKGDVVAD GASTDKGELA LGQNMLIAFM PWNGYNFEDS ILISERVVAE
DRYTSIHIEE LTVVARDTKL GPEEITRDIA SLGEAQLSRL DDSGIVYIGA EVEAGDVLVG
KVTPKGETQL TPEEKLLRAI FGEKASDVKD TSLRVPSGIA GTVIDVQVFT REGIERDKRA
QAIIDEHLRH YKLDLADQMR IVERDAFARV ERLITGKKAN GGPKKLAKGT VIDKVYLDSM
DPHHWFDIRL ADDEAAQQLE QVKDGLEQAR KDFDIAFEGK RKKLTQGDEL PPGVQKMVKV
YVAVKRRLQP GDKMAGRHGN KGVVSRILPV EDMPHMEDGS PVDIVLNPLG VPSRMNVGQI
LEVHLGLAAK GLGHKIGAML RAQSSAKEVR GFLDQIYNSS GKSENLDELN DVEVLEMAEN
LKGGVPFATP VFDGAKEEEI KAMLAMAGMP SSGQMTLFDG RTGEAFERKV TVGYMHYLKL
HHLVDDKMHA RSTGPYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGASY VLQEMLTVKS
DDVNGRTKVY ENIVKGEHRI EAGMPESFNV LVKEIRSLAI DIDLERY
