RPOB_DEHMB
ID RPOB_DEHMB Reviewed; 1273 AA.
AC A5FRK5;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN OrderedLocusNames=DehaBAV1_0578;
OS Dehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 /
OS BAV1).
OC Bacteria; Chloroflexi; Dehalococcoidia; Dehalococcoidales;
OC Dehalococcoidaceae; Dehalococcoides.
OX NCBI_TaxID=216389;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Pitluck S., Lowry S., Clum A.,
RA Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA Ritalahti K.M., Loeffler F., Richardson P.;
RT "Complete sequence of Dehalococcoides sp. BAV1.";
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABQ17163.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000688; ABQ17163.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_042377688.1; NC_009455.1.
DR AlphaFoldDB; A5FRK5; -.
DR SMR; A5FRK5; -.
DR PRIDE; A5FRK5; -.
DR KEGG; deb:DehaBAV1_0578; -.
DR PATRIC; fig|216389.18.peg.624; -.
DR HOGENOM; CLU_000524_4_3_0; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1273
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000329174"
FT REGION 1252..1273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1273 AA; 141514 MW; 72E53395C04C3616 CRC64;
MVSMLPKADA ATGVIRKSYA QLPEVVNVPN LIEMQLQSFV WFQEEGLREL IEEISPIKDF
VGNRLELEFI GYEFREPRLS EYECTQRDQT YSVPLYVKAR LIVKTTGEIK EPFDLFFGDI
PLMTALGTFI TSGTERVVVS QLLRSPGVYF TISDDPATGR PLCHTNLIPS RGAWLEFETS
NRDVISVKID GRRKIPVSTL LRAIGYSDDL DILNLFEVID NDPERHYIQS SIDRDPLIKD
EISALIDIYS RLRPGDPPNA DNARKLINEM FFDPQHYDLG KVGRYKVNRR LELPSREVGE
NRALTREDIV AIIQRIIMVN NGQDTPDDID HLGNRRIRTV GELVQNQFRI GLVRLERVAR
ERMSIVNLEM VTPSALVNIR PVVSAVKEFF GGSQLSQFMD QTNPLAEITN KRRLSAMGPG
GLSRERAGFD VRDVHYSHYG RICPIETPEG PNIGLIGSLA TYSRINRYGF VETPYRKVYS
KLKNNDKKLV GLKLKTEISE KGKVLAAAGS TISEDSFKII SKLPECDISV MPFVSAEVKY
MPADEEDRYI IAQANTRLDE KGYFLDDRIE ARSAERYVVE PPDKIDYMDV SPKQIFSVAA
SLIPFLEHDD ANRALMGANM QRQAVPLLRA EAPMVATGME REAARYSGQV IFAKHAGVAS
SVTSEKIIIR TAEGGHDEYL LKKFVRTNQG TCINQHAIIN KGQKIAAGQV LADSSATENG
ELALGQNCVV AFMSWQGFNY EDAIILSERL VREDAFTSIH ITKHELEARD TKLGVEEITR
DIPNVGEESL RELDEDGIIR IGAEVGPDDI LVGKITPKGE TELSAEEKLL RAIFGEKARE
VKDTSLRMPH GEWGKVINVR IFSRDSGDDL PARVNKWVQV WVAQKRKVSV GDKLAGRHGN
KGVISIIAPV EDMPYLPDGT PVDVVLNPIG VPSRMNLGQI LETHLGWAGH LLGFRVATPV
FDGADDTVIE DALARSWLSA KAGAIDMSPE NKRPSADAHK AIEWIKQQGF DGKKIFDEKH
PGLAKEVSLK LWLKDMGVDA SALSGAELEK KAYDVSSQSR LPSPIVGKSV LRDGRTGETF
DQPVTVGNMY ILKLIHLVED KVHARATGPY SLISQQPLGG KAQFGGQRFG EMEVWAMYAY
GTAHNLQEML TIKSDDIAGR AKAYESIVKG EDVLQPGVPE SFKVLVKELQ SLGLAVEVIN
EEVKIAPSEK VSSLNEGNLP ASDEISAEIL PETLYANTED ISEDSMMSVI DADDQDLVVS
SNDEEVSEND ERS
