RPOB_DESAH
ID RPOB_DESAH Reviewed; 1401 AA.
AC C0Q9X9;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=HRM2_36320;
OS Desulforapulum autotrophicum (strain ATCC 43914 / DSM 3382 / VKM B-1955 /
OS HRM2) (Desulfobacterium autotrophicum).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales;
OC Desulfobacteraceae; Desulforapulum.
OX NCBI_TaxID=177437;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43914 / DSM 3382 / VKM B-1955 / HRM2;
RX PubMed=19187283; DOI=10.1111/j.1462-2920.2008.01825.x;
RA Strittmatter A.W., Liesegang H., Rabus R., Decker I., Amann J., Andres S.,
RA Henne A., Fricke W.F., Martinez-Arias R., Bartels D., Goesmann A.,
RA Krause L., Puehler A., Klenk H.P., Richter M., Schuler M., Gloeckner F.O.,
RA Meyerdierks A., Gottschalk G., Amann R.;
RT "Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate
RT reducer oxidizing organic carbon completely to carbon dioxide.";
RL Environ. Microbiol. 11:1038-1055(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP001087; ACN16697.1; -; Genomic_DNA.
DR RefSeq; WP_015905447.1; NC_012108.1.
DR AlphaFoldDB; C0Q9X9; -.
DR SMR; C0Q9X9; -.
DR STRING; 177437.HRM2_36320; -.
DR EnsemblBacteria; ACN16697; ACN16697; HRM2_36320.
DR KEGG; dat:HRM2_36320; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_7; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000000442; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1401
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000214474"
SQ SEQUENCE 1401 AA; 156024 MW; C9899417085028D4 CRC64;
MTGSLLASKR IRKEFGSKSR VVDIPDLIGM QRTSFERFLQ RNVPPEERED IGIHLVFKSV
LPIKDFTGTS SLEYVSYSFG ETKHSMKECI SRGMTYEIPV NITVRLVVYD VDKDAGVTSI
RDIKEQEIYF GTIPLMTKRG TFIINGTERA VVSQLHRSSG VFFDHDKGKS HSSGKIIYTA
RIIPVRGSWI DMEIDPKDIV NIRIDRRRKF PVTILFKAFG YTNEDILSFF YKKEQIVKKD
GILFKEFNPD NLKRNRASFD ILDPETGEAV VKKGRLFTLR ALKQLQVAGL KSIPISEEDI
IGRGFATSVV HPETEEILAK AGSLIDPEVL EKIADAGITE FSILYVDTYS SDSMRKTLAV
DKVTSRAEAL IEIYRRLRPG NPATPEVAQD FIDHLFFKPA YYDLSNVGRL KMNHRLGMST
GIDVRTLRRE DILLTAATLV ELKDTQGAVD DIDHLGNRRV RAVGELLENQ YRIGLVRMER
AIKERMSMQE VDAMMPHDLV NPKPVSAVVR EFFGTSQLSQ FMDQTNPLSE TTHKRRLSAL
GPGGLTRERA GFEVRDVHPS HYGRICPIET PEGPNIGLIV SLCTYARVND FGFIETPYRI
AKDGVVSKQI THLSAFEEKE HPIAQANAVL DDQGHLMNDL VTSRVGGEFE MVAKNDIEFM
DVSPNQLVSV SASLIPFLEN DDANRALMGS NMQRQAVPLI RSEAPLVGTG IEGVVARDSG
VAIVAEHDGI VVDVDAKRIV VKNTENGKGS DDRTVTIYTA SKFVRSNQNT CFNHRPIVKK
GDAVRKGEVI ADGPSTEMGE LALGKNVTVA FMPWGGYNYE DSILVSERLV KEGVYTSVHV
EEFEVVARDT KLGKEEITRD IPNAGEEALK DLDDSGIIRL GAEVKPGDIL VGKITPKGET
QLSPEEKLLR AIFGEKAGDV KDTSLRVPPG VEGVVTAAKV FSRRGLPKDD RTRLIEDLEI
EKLEKDRDDE VRIITETIQE RLEDILAGQA LQAVVKKGKK VVVPAGTLVT RGLFEGISLT
DLMGLTVEDS SLTEMAHVIL EKAETQIKKA RENFNNQASR YEKGDDLPPG VLKLIKINVA
MKRVLSVGDK MAGRHGNKGV VSRILPVADL PYFEDGRTVD MVLNPLGVPS RMNVGQILEI
HLGCAARGLG RQIDDLIYEK KTDELRARIK RIFTTPLIKT GERANKDKTH YDFSSHYTVE
KSMAKRTNEE EIQFVDTIDH MDDTEFLEFA SMYKNGVHMA TPVFDGAAES EIKSLISYAG
LDPSGQSTLY DGRTGEPFDK PITVGIMYML KLHHLVDDKI HARSIGPYSL VTQQPLGGKA
QFGGQRLGEM EVWAMEAYGA AHALQEFITV KSDDMTGRTR MYEKIVKGQN VLEPGMPESF
RVLTKELQSL GLDINLLEGK K
