ID   RPOB_DINSH              Reviewed;        1378 AA.
AC   A8LM40;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Dshi_0268;
OS   Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Roseobacteraceae; Dinoroseobacter.
OX   NCBI_TaxID=398580;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16493 / NCIMB 14021 / DFL 12;
RX   PubMed=19741735; DOI=10.1038/ismej.2009.94;
RA   Wagner-Dobler I., Ballhausen B., Berger M., Brinkhoff T., Buchholz I.,
RA   Bunk B., Cypionka H., Daniel R., Drepper T., Gerdts G., Hahnke S., Han C.,
RA   Jahn D., Kalhoefer D., Kiss H., Klenk H.P., Kyrpides N., Liebl W.,
RA   Liesegang H., Meincke L., Pati A., Petersen J., Piekarski T.,
RA   Pommerenke C., Pradella S., Pukall R., Rabus R., Stackebrandt E., Thole S.,
RA   Thompson L., Tielen P., Tomasch J., von Jan M., Wanphrut N., Wichels A.,
RA   Zech H., Simon M.;
RT   "The complete genome sequence of the algal symbiont Dinoroseobacter shibae:
RT   a hitchhiker's guide to life in the sea.";
RL   ISME J. 4:61-77(2010).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000830; ABV92017.1; -; Genomic_DNA.
DR   RefSeq; WP_012176950.1; NC_009952.1.
DR   AlphaFoldDB; A8LM40; -.
DR   SMR; A8LM40; -.
DR   STRING; 398580.Dshi_0268; -.
DR   EnsemblBacteria; ABV92017; ABV92017; Dshi_0268.
DR   KEGG; dsh:Dshi_0268; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000006833; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1378
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000086368"
SQ   SEQUENCE   1378 AA;  153056 MW;  FF1B78710F7A8088 CRC64;
     MAQTVLGQKR FRKYYGKIRE VLEMPNLIEV QKSSYDLFLK SGDQPQPMDG EGIMGVFQSV
     FPIKDFNETA VLEFVKYELE KPKYDVEECQ QRDMTYSAPL KVTLRLIVFD VDEDTGAKSV
     KDIKEQDVFM GDMPLMTPNG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAC
     RIIPYRGSWL DFEFDAKDLV FARIDRRRKL PVTTLLYSLG MDQQDIMDAY YDTVTYTHRK
     GEGWVTKFFP ERIRGTRPTQ DVVDAATGEV IAEAGKKVTP RAVKQLIDEG NVSEILVPFD
     GIIGKFAAKD IINEENGAIY VEAGDELTWE VDKDGAVTGG TLKELLDAGI EEIPVLDIDN
     ITVGAYMRNT LAADKNMNRD TALMDIYRVM RPGEPPTVEA ASALFDTLFF DSERYDLSAV
     GRVKMNMRLA LDAEDTVRTL RKEDIVSCIK ALVDLRDGRG DIDDIDHLGN RRVRSVGELM
     ENQYRVGLLR MERAIKERMS SVEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP
     LSEVTHKRRL SALGPGGLTR ERAGFEVRDV HPTHYGRMCP IETPEGPNIG LINSLATFAR
     VNKYGFIETP YRRVEDGKVT DEVNYMSATE EMRHTVAQAN ANLDESGSFV NEMVNTRQSG
     EYTLAPRESV DLIDVSPKQL VSVAASLIPF LENDDANRAL MGSNMQRQAV PLLQADAPFV
     GTGIEGVVAR DSGAAIMARR GGFIDQVDAT RIVIRATEDL EPGDPGVDIY RLRKFQRSNQ
     NTCINQRPLV KVGDKVGKDD VIADGPSTDL GELALGKNVV VAFMPWNGYN YEDSILISER
     VARDDVFTSI HIEEFEVAAR DTKLGPEEIT RDIPNVGEEA LRNLDEAGIV YIGADVGPGD
     ILVGKITPKG ESPMTPEEKL LRAIFGEKAS DVRDTSMRLP PGDFGTVVEV RVFNRHGVEK
     DERALQIERE EVESLARDRD DEMAILERNI YARLKDMILG KTAVKGPKGV KPGSEITEDL
     LGTLSRGQWW QLALEDEAEA QMVEALNQQY EAQKRALTAR FEDKVEKVRR GDDLPPGVMK
     MVKVFIAVKR KLQPGDKMAG RHGNKGVISK VVPMEDMPFL ADGTPVDFVL NPLGVPSRMN
     VGQILETHMG WAARGMGLKI DEALGEYRRS GDMTPVREAM RLAYGDEAYE EAISGLDQDD
     LLERAGNVTR GVPIATPVFD GAKEADVNDA LTRAGFDTSG QSDLYDGRTG EKFARQVTVG
     VKYLLKLHHL VDDKIHARST GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAYTLQ
     EMLTVKSDDV AGRTKVYESI VKGEDNFEAG VPESFNVLVK EVRGLGLNME LLDAEGEE