ID   RPOB_DRIGR              Reviewed;        1071 AA.
AC   Q06H05;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Drimys granadensis.
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Magnoliidae; Canellales; Winteraceae; Drimys.
OX   NCBI_TaxID=224735;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17020608; DOI=10.1186/1471-2148-6-77;
RA   Cai Z., Penaflor C., Kuehl J.V., Leebens-Mack J., Carlson J.E.,
RA   dePamphilis C.W., Boore J.L., Jansen R.K.;
RT   "Complete plastid genome sequences of Drimys, Liriodendron, and Piper:
RT   implications for the phylogenetic relationships of magnoliids.";
RL   BMC Evol. Biol. 6:77-77(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; DQ887676; ABH88289.1; -; Genomic_DNA.
DR   RefSeq; YP_784378.1; NC_008456.1.
DR   AlphaFoldDB; Q06H05; -.
DR   SMR; Q06H05; -.
DR   GeneID; 4363553; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1071
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000276587"
SQ   SEQUENCE   1071 AA;  120597 MW;  91419F808F6A1FB4 CRC64;
     MLRDGNEGMF TIPGFSQIQF EGFFRFIDQG LTEELHKFPK IEDTDQEIEF QLFVDTYRLV
     EPLIKERDAV YESLTYSSEL YVPAGLIWKT GRDMQEQTIF IGNIPLMNSL GTSIVNGIYR
     IVINQILQSP GIYYRSELDH NGISVYTGSI ISDWGGRSEL EIDRKARIWA RVSRKQKISI
     LVPSSAMGSN LREILDNVCY PEIFLSFPNE KEKKKIGSRE NAILEFYQQF ACVGGDPVFS
     ESLCKELQKK FFQQRCELGR IGRRNMNWRL NLDIPQNNLF LLPRDVLAAA DHLIGMKFGM
     GTLDDMNHLK NKRIRSVADL LQDQFGLALV RLENVVRGTI CGAIRHKLIP TPHNLVTSTP
     LTTTYESFFG LHPLSQVLDR TNPLTQIVHG RKSSYLGPGG LTGRTASFRI RDIHPSHYGR
     ICPIDTSEGI NVGLIGSLAI HARIGYLGSI ESPFYEISQR SKEVQTVYLS PNRDEYYMVA
     AGNSLALNQG IQEEQVVPAR YRQEFLTIAW EQIHLRSIFP FQYFSIGASL IPFIEHNDAN
     RALMSSNMQR QAVPLSRSEK CIVGTGLERQ AALDSGVSAI AEQDGKIIYT DTDKIVLSGN
     GDTAISIPLV IYQRSNKNTC MHQKPQVPRG KCLKKGQILA DGAATVGGEL ALGKNVLVSY
     MPWEGYNFED AVLISERLVY GDIYTSFHIR KYEIQTHVTS QGPERITNEI PHLEAHLLRN
     LDINGIVMLG SWIETGDILV GKLTPQTAKE SSYAPEDRLL RAILGIQVST AKETCLKLPI
     GGRGRVIDVR WIQKGGGSSY NPEMIRVYIS QKREIKVGDK VAGRHGNKGI ISKILPRQDM
     PYLQDGTPVD MVFNPLGVPS RMNVGQIFEC SLGLAGDLLD RHYRIAPFDE RYEQEASRKL
     VFPELYEASK QTANPWVFEP EYPGKSRIFD GRTGDPFEQP VIIGKSYILK LIHQVDDKIH
     GRSSGHYALV TQQPLRGRAK QGGQRVGEME VWALEGFGVA HISQEMLTYK SDHIRARQEV
     LGTTIIGGTI PNPEDAPESF RLLVRELRSL ALELNHFLVS EKNFQIHRKE A