ID   RPOB_EHRRW              Reviewed;        1380 AA.
AC   Q5HC05; Q5FCV5;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN   OrderedLocusNames=Erum1720, ERWE_CDS_01710;
OS   Ehrlichia ruminantium (strain Welgevonden).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Anaplasmataceae; Ehrlichia.
OX   NCBI_TaxID=254945;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Welgevonden;
RX   PubMed=15637156; DOI=10.1073/pnas.0406633102;
RA   Collins N.E., Liebenberg J., de Villiers E.P., Brayton K.A., Louw E.,
RA   Pretorius A., Faber F.E., van Heerden H., Josemans A., van Kleef M.,
RA   Steyn H.C., van Strijp M.F., Zweygarth E., Jongejan F., Maillard J.C.,
RA   Berthier D., Botha M., Joubert F., Corton C.H., Thomson N.R., Allsopp M.T.,
RA   Allsopp B.A.;
RT   "The genome of the heartwater agent Ehrlichia ruminantium contains multiple
RT   tandem repeats of actively variable copy number.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:838-843(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Welgevonden;
RX   PubMed=16547041; DOI=10.1128/jb.188.7.2533-2542.2006;
RA   Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y.,
RA   Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.;
RT   "Comparative genomic analysis of three strains of Ehrlichia ruminantium
RT   reveals an active process of genome size plasticity.";
RL   J. Bacteriol. 188:2533-2542(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CR767821; CAH57888.1; -; Genomic_DNA.
DR   EMBL; CR925678; CAI26665.1; -; Genomic_DNA.
DR   RefSeq; WP_011154856.1; NC_006832.1.
DR   AlphaFoldDB; Q5HC05; -.
DR   SMR; Q5HC05; -.
DR   STRING; 254945.Erum1720; -.
DR   PRIDE; Q5HC05; -.
DR   EnsemblBacteria; CAI26665; CAI26665; ERWE_CDS_01710.
DR   GeneID; 56784883; -.
DR   KEGG; eru:Erum1720; -.
DR   KEGG; erw:ERWE_CDS_01710; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_5; -.
DR   OMA; FMTWEGY; -.
DR   BioCyc; ERUM254945:ERUM_RS00940_1-MON; -.
DR   Proteomes; UP000001021; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1380
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224056"
SQ   SEQUENCE   1380 AA;  155933 MW;  40641E1FF6302A8D CRC64;
     MSSSVTSKYV LNSFSSVPRL SYAKSIDIKD SLTDLIKIQR DSYNAFIGID QDVDSGIKNI
     FQSMFPIQDL LGRAVLQFVS YSIGEPQYDE YECIKRGITY SVPIRIVLRF IVWKVQEVSF
     KEVKYVVDEE TSEKSIKYIK EQEVSIGDLP TMTSYGTFII NGVERVIVSQ MHRSPGVFFD
     SDKGKTYSSG KLIYLARIIP YRGSWLDFEF DIKDILYFRI DRKRKLPVSL LLRALGLSNS
     EILDTFYDKI RYERCENGWV VPFVVDRFRG VRLSYDLVDI DGNVLVKANT RITLRLAKKL
     ASDGLKKYLV PFAEIQGLFI ANDLVDPASN VMIMCAGESI TSEHINKLKL FDINEIFILN
     IDFLTVGPYI LNTLFLDKNI SYEDALFEIY KVLRSGESPS LDTMKAFFDG LFFEKERYDL
     STVGRIKLND HLGLDISEDV TVLTKDDIIH VIKKLVLLRD GEGFVDDIDH LGNRRVRSVG
     EFIENQFRIG ILRLERMIMD YMSSVNFDNA MPCDFVNPKV LATVLKDFFS SSQLSQFMDQ
     TNPLSEVTHK RRLSALGPGG LTRERAGFEV RDVHPTHYGR ICPIETPEGQ NIGLISSLAI
     YARINKHGFI ESPYRKVDNG VVTDKVEYLL AMQESNYYIA DASATLDENN RFVDDMLYCR
     HDGNFVMVKR EQVDYIDVSP KQIVSVAASL IPFLENNDAN RALMGSNMQR QAVPLLKADA
     PLVGTGMESI VAAGSGTVVL AKRSGIVHRV DGLYIVIRAF DKEKNEYLGV DIYNLRKFQR
     SNHNTCINQK PLVKPGDYVR ENDVIADGSA IDQGELALGK NVLVAFMSWQ GYNFEDSIVI
     SSEVVKKDVF TSIHIEEFEC VVRDTALGPE KIMRSIPDVN EDSLSHLDDV GIVNVGAEVS
     AGDILVGKVT PRPPVSLPPE TKLLVTIFGE KVFDCVDSSL YLPIDVEGTV VDVHVFVRRG
     VEENDRSLLI KQNEINGFIK ERDYEIDVVS EYFYDELKRV LVNTNTEYNN QNIEDYLKSI
     PQKSWWDIKL SDESVLSQIS DLKEKFDSMI ENAHSKFDQK IDKLNYGYDL PQGVLCIVKV
     FVAVKHNLQP GDKMAGRHGN KGVISRIVPV EDMPYLEDGT PVDIILNSLG VPSRMNVGQI
     LETHLGWASV NLGKKIGNIL DNIDELTIAH LRNFLDQVYD GQDLKYSIRS MSDDDLLAFA
     ERLRDGVPMA APVFEGPKDN QISNLLKLAD LDVSGQVDLY DGRIGEKFDR KVTVGYIYML
     KLHHLVDDKI HARSVGPYGL VTQQPLGGKS HFGGQRFGEM ECWALQAYGA AYTLQEMLTV
     KSDDIVGRVK IYESIIKGDS NFECGIPESF NVMVKELRSL CLDVALKQDK DFLHDRKINN