ID   RPOB_ENTFA              Reviewed;        1207 AA.
AC   Q82Z40;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=EF_3238;
OS   Enterococcus faecalis (strain ATCC 700802 / V583).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=226185;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA   Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA   DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA   Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA   Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT   faecalis.";
RL   Science 299:2071-2074(2003).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE016830; AAO82906.1; -; Genomic_DNA.
DR   RefSeq; NP_816836.1; NC_004668.1.
DR   RefSeq; WP_002354775.1; NZ_KE136524.1.
DR   AlphaFoldDB; Q82Z40; -.
DR   SMR; Q82Z40; -.
DR   STRING; 226185.EF_3238; -.
DR   PRIDE; Q82Z40; -.
DR   EnsemblBacteria; AAO82906; AAO82906; EF_3238.
DR   KEGG; efa:EF3238; -.
DR   PATRIC; fig|226185.9.peg.3018; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000001415; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1207
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047898"
SQ   SEQUENCE   1207 AA;  135188 MW;  F92836D1BE35F218 CRC64;
     MKSLAGHVVK YGKHRERRSF ARISEVLELP NLIEIQTDSY QWFLDEGLRE MFEDILPIDD
     FQGNLSLEFV DYELKEPKYT VEEARAHDAN YSAPLHVTLR LTNRETGEIK SQEVFFGDFP
     LMTEMGTFII NGAERVIVSQ LVRSPGVYFH GKVDKNGKEG FGSTVIPNRG AWLEMETDAK
     DISYVRIDRT RKIPLTVLVR ALGFGSDDTI FEIFGDSESL RNTIEKDLHK NASDSRTEEG
     LKDIYERLRP GEPKTADSSR SLLTARFFDP KRYDLANVGR YKVNKKLDLK TRLLNLTLAE
     TLVDPETGEI IVEKGTVLTH QIMETLGEYI DNGLNSVTYY PSEDAVVTEP MTIQVIQVLS
     PKDPERIVNV IGNGYPDDSV KTVRPADIVA SMSYFFNLME DIGNVDDIDH LGNRRIRSVG
     ELLQNQFRIG LARMERVVRE RMSIQDTETL TPQQLINIRP VVASIKEFFG SSQLSQFMDQ
     TNPLGELTHK RRLSALGPGG LTRDRAGYEV RDVHYSHYGR MCPIETPEGP NIGLINSLSS
     YAKVNKFGFI ETPYRRVDRA TGRVTDQVDY LTADIEDHYI VAQANSLLNE DGTFANDVVM
     ARLQSENLEV AVDKVDYMDV SPKQVVAVAT ACIPFLENDD SNRALMGANM QRQAVPLIQP
     RSPWVGTGME YKSAHDSGAA LLCKHDGVVE FVDAKEIRVR RDNGALDKYM VTKFRRSNSG
     TSYNQRPIVH LGEKVEKGDT LADGPSMEEG EMALGQNVLV AFMTWEGYNY EDAIIMSRRL
     VKDDVYTSVH IEEYESEARD TKLGPEEITR EIPNVGEDAL KDLDEMGIIR IGAEVQDGDL
     LVGKVTPKGV TELSAEERLL HAIFGEKARE VRDTSLRVPH GGGGIVHDVK IFTREAGDEL
     SPGVNMLVRV YIVQKRKIHE GDKMAGRHGN KGVVSRIMPE EDMPFLPDGT PVDIMLNPLG
     VPSRMNIGQV LELHLGMAAR QLGIHVATPV FDGATDEDVW ETVREAGMAS DAKTVLYDGR
     TGEPFDNRIS VGVMYMIKLA HMVDDKLHAR SIGPYSLVTQ QPLGGKAQFG GQRFGEMEVW
     ALEAYGAAYT LQEILTYKSD DVVGRVKTYE AIVKGEPIPK PGVPESFRVL VKELQSLGLD
     MRVLDIEEAE IELRDMDDDD DDLITVDALT KFAEQQSAKQ LEKEAESVVK EEAQDVVQEI
     ETAEDRD