ID   RPOB_EUGGR              Reviewed;        1082 AA.
AC   P23579;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Euglena gracilis.
OG   Plastid; Chloroplast.
OC   Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC   Euglenales; Euglenaceae; Euglena.
OX   NCBI_TaxID=3039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Z / UTEX 753;
RX   PubMed=2110656; DOI=10.1093/nar/18.7.1869;
RA   Yepiz-Plascencia G.M., Radebaugh C.A., Hallick R.B.;
RT   "The Euglena gracilis chloroplast rpoB gene. Novel gene organization and
RT   transcription of the RNA polymerase subunit operon.";
RL   Nucleic Acids Res. 18:1869-1878(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Z / UTEX 753;
RX   PubMed=8346031; DOI=10.1093/nar/21.15.3537;
RA   Hallick R.B., Hong L., Drager R.G., Favreau M.R., Monfort A., Orsat B.,
RA   Spielmann A., Stutz E.;
RT   "Complete sequence of Euglena gracilis chloroplast DNA.";
RL   Nucleic Acids Res. 21:3537-3544(1993).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; X17191; CAA35052.1; -; Genomic_DNA.
DR   EMBL; X70810; CAA50138.1; -; Genomic_DNA.
DR   PIR; S09210; RNEGB.
DR   RefSeq; NP_041951.1; NC_001603.2.
DR   AlphaFoldDB; P23579; -.
DR   SMR; P23579; -.
DR   PRIDE; P23579; -.
DR   GeneID; 807537; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1082
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048022"
SQ   SEQUENCE   1082 AA;  124532 MW;  27CD37CE308B22BA CRC64;
     MVNGCRVRSN LLDIQRNSFR SFLKKGLVEE LRKIKDIAHE GFRISFQTDN VKYKKPKISA
     EFALKNGETY SLSVHIPVEV TYNNMFLVRN KYILFAKIPL MTEKGTFIFN GNKRIMVNQI
     IRSPGVYFEK NRYNDSIFAT LIPTFGSWLT FKIDQDEGIF VKVDKIKTAI PLINFLKCLG
     LSRKKFFLYL NDPIFIETLQ ESESYGIRLE FFEFYKIFFP NEVNVRFGNA RKFLRSKFMD
     PRKYDLGEVG RFRVNTKIYR SEFFQSNRTL QPEDVLGIAH YLIELKKGMI PFDEIDDLKN
     KLVRSIGELL QSQFRIILNE LESSLKEKLI FLYKNPSEKT FRLSRFFNSY FITNRIRKFF
     SVNPLSQLLD DTNSLSELTH KRKLSPFGPN GLNKERTKLD VREINTSQYG RVCPIETSEG
     KNAGLILSLA KDVRVDKYGF LESPFYKVLR GKIETNKGIY FISSAQEKYF TVAPFDVFRS
     SQSNLLDKNK LLGVKRSKIF SYSFSKNIDF ISISTDQFTS LGTGLVPFLE HNDANRVLMG
     SNMQRQSLIL LEKEVPFIKT GREALINRES DATVLAKSSG KVIYSSLKKI VIQEEEYCAN
     IEFFNKNYSF FNLLGCLNEI SQKNFKLIKK VNQKIYFLES PKKSNHGVYI QKIPIVHEGE
     WVRKGQIIAD GMSTLRGGIC LGKNVLVAYL GWEGYNFEDA VIISERLVFE DIFTSIHMKK
     FKTFIVNDEK KGENISMFIP NASLKTIKNL KNNGIIKIGS EIKAQDVLIG RIKVKLKNTP
     KNKMLIAFFG NKVRKDVSLR SPRSLVGIVT SVEILCKKSN CSVLIHVAEK RRIQIGDKIA
     GRHGNKGIIS KIVPSIDMPF LPDGTPVDMI LNPLGIPSRM NVGQVFESLL NLSSLFLKER
     YKIQPFDEVQ TSMNSKSFVY KKLNEARKRT KKDWLFNPNY PGKAFLYDGR NCRPFDHPVA
     FGYAYILKLI HMVKDKIHAR VTGPYSSVTQ QPLRGKSKNG GQRFGEMEVW AIEGFGAAYL
     LQELLTIKSD DVLNRSEALF SLINGTYFSK PNIPEAFKLF ILEMQSLCID IKIFTNNYKK
     FD