RPOB_EUGLO
ID RPOB_EUGLO Reviewed; 1076 AA.
AC P27059;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Euglena longa (Euglenophycean alga) (Astasia longa).
OG Plastid; Non-photosynthetic plastid.
OC Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC Euglenales; Euglenaceae; Euglena.
OX NCBI_TaxID=3037;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCAP 1204-17a;
RX PubMed=11212895; DOI=10.1078/s1434-4610(04)70033-4;
RA Gockel G., Hachtel W.;
RT "Complete gene map of the plastid genome of the nonphotosynthetic euglenoid
RT flagellate Astasia longa.";
RL Protist 151:347-351(2000).
RN [2]
RP PRELIMINARY NUCLEOTIDE SEQUENCE OF 1-524.
RC STRAIN=CCAP 1204-17a;
RX PubMed=2078869; DOI=10.1007/bf00309917;
RA Siemeister G., Buchholz C., Hachtel W.;
RT "Genes for ribosomal proteins are retained on the 73 kb DNA from Astasia
RT longa that resembles Euglena chloroplast DNA.";
RL Curr. Genet. 18:457-464(1990).
RN [3]
RP SEQUENCE REVISION.
RC STRAIN=CCAP 1204-17a;
RX PubMed=7859309; DOI=10.1007/bf00309557;
RA Gockel G., Hachtel W., Baier S., Fliss C., Henke M.;
RT "Genes for components of the chloroplast translational apparatus are
RT conserved in the reduced 73-kb plastid DNA of the nonphotosynthetic
RT euglenoid flagellate Astasia longa.";
RL Curr. Genet. 26:256-262(1994).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AJ294725; CAC24573.1; -; Genomic_DNA.
DR PIR; S23207; S23207.
DR RefSeq; NP_074962.1; NC_002652.1.
DR AlphaFoldDB; P27059; -.
DR SMR; P27059; -.
DR PRIDE; P27059; -.
DR GeneID; 1457304; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1076
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000048012"
SQ SEQUENCE 1076 AA; 124363 MW; 5D23D488E3EB2D76 CRC64;
MINRLKTYSN LLNIQSKSFK NFLKIGIINE LKKIQKIIHS NFEINFHINA LKYKKPKLSS
ESCLIKNETY NIDIKIPMEI KYEGKIIFKK KYISFCKIPF MTEKGTFICN GNTRIIINQL
IRSPGIYIKK KKNCLLATLI PKTGSWITIK RNKKKENFIK IDKINNSIPL FTFLNILGLS
KKKITLSLNK NNYSKNFKIS KKKKIEIPTI NLTQLSKENN IKTIRNNLYN RFLNSDNYNL
GDTGRFKINK KIYKTEFFTN KKILMPEDFL GIFNYMIKIK NINIKSNKID DLKNKIVLSV
GELMQNKFNN IIKDIYTKII EKINKFEQKK KQEEKYNKEK KEEKIKINKT YFINSKNLTD
NIKKFITTNP LSQLLNDLNP LSELTHKRKI STLGIGGIEK NKASTKIREI HNSHYGRICP
IETSEGKNAG LVLSLAKDIR INKHGFIESP FYKVIKGNIK KNKGIFFISS ENEKNIKIAP
CDILKNFKLN KNYGVKNKNE FYYDSYKSIN FISTSTDQFN SIGTGIIPFL EHNDANRVLM
GATMQKQALI LKNKEPSLIE TGREILINRD SKSTIIAKES GTVIYSSSKK IIIQKKSKKS
KFKNINLFKK YFKKKLKKNK KIKKHKHTIY LLNIDEKNNS NIYQTRTSIV KKNDWIKKGQ
IIAEGIGSLN GNLCLGKNIL VGYLSWEGYN FEDAIIISER LRNEDILTSI HVKKCKSFLI
NHKEKKEEIT KNIPDIKLKN VKNLNNNGII KIGSKINGKE ILIGIIKKRL INYEVELIYE
ILSEYERKNI SVLSPKNLVG IVTNTKIHKI KNCYQIEIYI TEKKKIQIGD KLSGRHGNKG
VISKIIPIVD MPYLPDGTPI DIILNPLGIP SRMNVGQIYE CLLNLSAINL KERYKIQPFD
EYQYKNNSEI LIYKKLNQSR KKTKKEWLFN PNNPGKTILF NGKNGKPFKQ TISFGYSYIL
KLMHIAEEKI HAKTISNYSS IIKQPVKGKS KNGGQRFGEM EVWAIEGFGA AFLLQELLTI
KSDDLHNKSQ ILKNLMNGLS MSKPNVPESF KLLILELQSL GLNINIFTKK KTLFTK
