ID   RPOB_EXISA              Reviewed;        1182 AA.
AC   C4KZQ4;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=EAT1b_1641;
OS   Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b).
OC   Bacteria; Firmicutes; Bacilli; Bacillales;
OC   Bacillales Family XII. Incertae Sedis; Exiguobacterium;
OC   unclassified Exiguobacterium.
OX   NCBI_TaxID=360911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1283 / AT1b;
RX   PubMed=21460088; DOI=10.1128/jb.00303-11;
RA   Vishnivetskaya T.A., Lucas S., Copeland A., Lapidus A., Glavina del Rio T.,
RA   Dalin E., Tice H., Bruce D.C., Goodwin L.A., Pitluck S., Saunders E.,
RA   Brettin T., Detter C., Han C., Larimer F., Land M.L., Hauser L.J.,
RA   Kyrpides N.C., Ovchinnikova G., Kathariou S., Ramaley R.F., Rodrigues D.F.,
RA   Hendrix C., Richardson P., Tiedje J.M.;
RT   "Complete genome sequence of the Thermophilic Bacterium Exiguobacterium sp.
RT   AT1b.";
RL   J. Bacteriol. 193:2880-2881(2011).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP001615; ACQ70567.1; -; Genomic_DNA.
DR   RefSeq; WP_012727685.1; NC_012673.1.
DR   AlphaFoldDB; C4KZQ4; -.
DR   SMR; C4KZQ4; -.
DR   STRING; 360911.EAT1b_1641; -.
DR   PRIDE; C4KZQ4; -.
DR   EnsemblBacteria; ACQ70567; ACQ70567; EAT1b_1641.
DR   KEGG; eat:EAT1b_1641; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000716; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1182
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000214478"
FT   REGION          1150..1182
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1165..1182
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1182 AA;  131840 MW;  56AD4CDBE5B458DF CRC64;
     MTGQLVQYGR HRQRRSFARI SEVLELPNLI EIQTASYEWF LREGLKEMFT DISPISDFTG
     NLVLEFIDYS LSEPKYSIDE SKERDVTYSA PLRVKVRLQN KETGELKEQE VFMGDFPLMT
     ESGTFIINGA ERVIVSQLVR SPSVYYNAKL DKNGKRGFGA TVIPNRGAWL ELETDAKDIV
     YVRIDRTRKI PVTVLLRALG FGTDQEIIDL LGDDEYLRNT LEKDNTESTE KALIEIYERL
     RPGEPPTVEN AKSLLVSRFF DPKRYDLANV GRYKMNKKLH LKNRLFGQKL AETLVDPETG
     EVLAEAGTIL DRRNLDRILP HLENGLGYID AEPTGGVAEG EPFGLQSIKV ISPDDPDGER
     ILNIIGNGNI DRSVKHITPA DIIASINYFF NLLHEVGTTD DIDHLGNRRL RSVGELLQNQ
     FRIGLSRMER VVKERMSIQD QNAITPQALI NIRPVIASLK EFFGSSQLSQ FMDQTNPLAE
     LTHKRRLSAL GPGGLTRERA GFEVRDVHYS HYGRMCPIET PEGPNIGLIN SLSSYAKVNE
     YGFIEAPYRR VDPETGLVTG DIQYMTADEE DLYVVAQANM PLTEDGHFAD EQVLCRFRGQ
     NLSVEPSRVD YMDVSPKQVV SAATACIPFL ENDDSNRALM GANMQRQAVP LLQPDSPIVG
     TGMEYVSAKD SGAAIVAKHP GVVERVTARE ILIRRTSDVN GSEVSGDLDR YKLQKYVRSN
     QGTCYNQKPI IAAGDRVEKG EILADGPSMD MGELALGRNV VVAFMTWDGY NYEDAIIMSE
     RLVKDDVYTS IHIEEYESES RDTKLGPEEI TRDIPNVGDD ALRNLDDRGI IRIGAEVKDG
     DILVGKVTPK GVTELTAEER LLHAIFGEKA REVRDTSLRV PNGGDGIILD VKVFDRENGD
     ELSPGVNQMV RVYIVQKRKI HEGDKMAGRH GNKGVISRIL PEEDMPYMPD GTPVDIMLNP
     LGVPSRMNIG QVLELHLGMA AKKLGIKVAT PVFDGAREED VWATIEEAGM DKDAKTRLYD
     GRTGEPFDNR VSVGVMYMIK LAHMVDDKLH ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME
     VWALEAYGAA YTLQEILTIK SDDTVGRVKA YEAIVKGESV PKAGVPESFR VLIKELQALG
     MEVKMMSADD EEVEMKDEDD DNIPNATSAL EQVVQPTVTE EE