RPOB_FLAJ1
ID RPOB_FLAJ1 Reviewed; 1270 AA.
AC A5FIJ3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Fjoh_1943;
OS Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC
OS 14942 / NCIMB 11054 / UW101) (Cytophaga johnsonae).
OC Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=376686;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101;
RX PubMed=19717629; DOI=10.1128/aem.01495-09;
RA McBride M.J., Xie G., Martens E.C., Lapidus A., Henrissat B., Rhodes R.G.,
RA Goltsman E., Wang W., Xu J., Hunnicutt D.W., Staroscik A.M., Hoover T.R.,
RA Cheng Y.Q., Stein J.L.;
RT "Novel features of the polysaccharide-digesting gliding bacterium
RT Flavobacterium johnsoniae as revealed by genome sequence analysis.";
RL Appl. Environ. Microbiol. 75:6864-6875(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000685; ABQ04975.1; -; Genomic_DNA.
DR RefSeq; WP_012024015.1; NZ_MUGZ01000012.1.
DR AlphaFoldDB; A5FIJ3; -.
DR SMR; A5FIJ3; -.
DR STRING; 376686.Fjoh_1943; -.
DR EnsemblBacteria; ABQ04975; ABQ04975; Fjoh_1943.
DR KEGG; fjo:Fjoh_1943; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_10; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000006694; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 3.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1270
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000329177"
SQ SEQUENCE 1270 AA; 142684 MW; 2718A7398EF9D1CA CRC64;
MITNQTERLN FASTKNIPDY PDFLDVQVKS FKDFFQLETK SDERGNEGLY NTFMENFPIT
DTRNNFVLEF LDYFVDPPRY TIQECIERGL TYSVPLKARL KLYCTDPEHE DFETIVQDVY
LGTIPYMTPS GTFVINGAEC VVVSQLHRSP GVFFGQSFHA NGTKLYSARV IPFKGSWIEF
STDINSVMYA YIDRKKKLPV TTLFRAIGFE RDKDILEIFD LAEEIKVSKT GIKKYIGRRL
AARVLNTWHE DFVDEDTGEV VSIERNEIIL DRDTIIDKDN VEEIIDSNVK SILLHKEDNN
QADYAIIHNT LQKDPTNSEK EAVEHIYRQL RNAEPPDEET ARGIIDKLFF SDQRYNLGEV
GRYRMNKKLD LDIPMDKQVL TKEDIITIVK YLIELINSKA EIDDIDHLSN RRVRTVGEQL
SQQFGVGLAR MARTIRERMN VRDNEVFTPI DLINAKTLSS VINSFFGTNQ LSQFMDQTNP
LAEITHKRRL SALGPGGLSR ERAGFEVRDV HYTHYGRLCP IETPEGPNIG LISSLGVYAK
VNGMGFIETP YRKVTDGVVD LESAPIYLSA EEEEGKMIAQ ANIEMDASGK ITASNVIARE
EGDFPVVEPS SVHYTDVAPN QIASISASLI PFLEHDDANR ALMGSNMMRQ AVPLIRPEAP
IVGTGLERQV ASDSRVLINA EGHGTVEYVD ANIITIKYDR TEDERMVSFD ADEKTYNLIK
FRKTNQGTSI NLKPIVRRGD RVVPGQVLSE GYATQNGELA LGRNLKVAFM PWKGYNFEDA
IVISEKVVRD DIFTSIHVDD YSLEVRDTKL GNEELTNDIP NVSEEATKDL DENGMIRIGA
EVKPGDILIG KITPKGESDP TPEEKLLRAI FGDKAGDVKD ASLKASPSLH GVVLDKKLFA
RAVKDKRKRT QDKDALGALE MEFETKFVEL KDRLVEKLFL IVNGKTSQGV MNDLGEEVLP
KGKKYTQKML YAVEDFAHLS KGQWVADDAT NKMVNDLIHN YKIKLNDLQG ALRREKFTIT
VGDELPSGIL KLAKIYIAKK RKLKVGDKMA GRHGNKGIVA RIVRHEDMPF LEDGTPVDIV
LNPLGVPSRM NIGQIYETVL GWAGMNLGRK FATPIFDGAS LDEINALTDE ANVPRFGHTY
LYDGGTGERF AQKATVGVIY MLKLGHMVDD KMHARSIGPY SLITQQPLGG KAQFGGQRFG
EMEVWALEAY GASSTLREIL TVKSDDVIGR AKTYEAIVKG ETMPEPGLPE SFNVLMHELK
GLGLDLRLEE
