RPOB_FRAP2
ID RPOB_FRAP2 Reviewed; 1358 AA.
AC B0TX10;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Fphi_1046;
OS Francisella philomiragia subsp. philomiragia (strain ATCC 25017 / FSC 153 /
OS O#319-036).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC Francisellaceae; Francisella.
OX NCBI_TaxID=484022;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25017 / FSC 153 / O#319-036;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Richardson P.;
RT "Complete sequence of chromosome of Francisella philomiragia subsp.
RT philomiragia ATCC 25017.";
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000937; ABZ87268.1; -; Genomic_DNA.
DR RefSeq; WP_012280433.1; NC_010336.1.
DR AlphaFoldDB; B0TX10; -.
DR SMR; B0TX10; -.
DR STRING; 484022.Fphi_1046; -.
DR PRIDE; B0TX10; -.
DR EnsemblBacteria; ABZ87268; ABZ87268; Fphi_1046.
DR GeneID; 64050512; -.
DR KEGG; fph:Fphi_1046; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_6; -.
DR OMA; FMTWEGY; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1358
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000086370"
SQ SEQUENCE 1358 AA; 151454 MW; 5136EA0306CA71AF CRC64;
MSYSYAEKKR IRKEFGVLPH ILDVPYLLSI QTESYRKFLN ADDAKGRLHS GLELVLKQSF
PVESKNGQYE LHYVDYQIGE PTFDETECQV RGATYDAPLN VKLRLVVYNK EALPSEKVVE
DIREEYVYMG DIPLMTTNGT FIINGTERVV VSQLHRSPGV FFSKDDSEEG AFSARIIPYR
GSWLDFEFDS KGIIWARIDR KRKFCATVIL KALGYTQEEI LNRFSDSKTI SFNNKGFALK
LDDLSSMKGE VLKFDIIDEK DNTIVKKNKK LTSRDIKKIK DAGVDSVAID FDLVSTLRVA
KDIVNEATGE VIAYANDDVT ENLLEACVEA GMLELEVIDF ITTERGRYIS DTLKYDLTKN
TDEALVEIYK VLRPGDPPAA ASVKALFEGL FFIESRYSLS DIGRMKLNAR LGSDNVSKDI
YTLENSDIVG VIEELINIRD GKGKVDDIDH LGNRRVRSVG EMVENQFRIG LYRVEKGIRE
SMSLVHKDKL MPKDIVNSKP ITAAIKEFFT SGALSQFMDQ DNPLSEVTHK RRISALGPGG
LSRDRAGFEV RDVHATHYGR LCPIETPEGP NIGLINSLAS YARVNDYGFL EAPYRKVVNG
KVTNEIEYLS AIDEDHYVIA QASTKLDKNN HFVEDLIQCR SGGEAIFTES SRVQYMDVSA
KQMVSAAAAL IPFLEHDDAN RVLMGANMQR QAVPTLKSEK PLVGTGMEKI VARDSGNCII
ARNAGEIAEV DSNRIVVKVD TEKSKTSKLV DIYSLTKFKR SNKNTCINQR PIVNVGDKIE
AGDILADGFA TDFGELSLGH NLMVAFMPWN GYNFEDSILL SERIVKDDKY TSIHIEEFTC
VARDTKLGPE EVTADIPNVS ESSLAKLDES GIVHIGANVE AGDILVAKIT PKAEQQLTPE
ERLLRAIFNE KASNVADSSL RMPSGTSGTV INVQVFENDK GGKSKRALKI EKELIDKARK
DFDEEFAVIE SVVKSSIETD VIGAKIQKAK GLKKGAVLTK EFLATLPFSK WLEISFEDEK
LEQKVQNARE YYEEAKIAVD ARFEAKKKSI MQSNELSPGV LKTVKVFVAI KKRIQPGDKM
AGRHGNKGVV SRVLPVEDMP YMEDGTPVDV CLNPLGIPSR MNIGQILEAH LGLASYGLGK
KIEQTLEKTR KAAELRKTLE EVYNSVGDKK VDLKALNDEE ILNLCENLKG GVPIATPVFD
GAKEEDIKSL LKIGGFATNG QMKLFDGRTG KPFDRHVTVG YMYMLKLDHL VDDKMHARST
GSYSLVTQQP LGGKAQFGGQ RFGEMEVWAL QAYGAAYTLR EMLTVKSDDI TGRSKMYKNI
VDGKLTMNVD VPESFNVLRN EVRALGIDMD FDYSSEEE
