RPOB_FRATH
ID RPOB_FRATH Reviewed; 1358 AA.
AC Q2A1M7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=FTL_1744;
OS Francisella tularensis subsp. holarctica (strain LVS).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC Francisellaceae; Francisella.
OX NCBI_TaxID=376619;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVS;
RA Chain P., Larimer F., Land M., Stilwagen S., Larsson P., Bearden S.,
RA Chu M., Oyston P., Forsman M., Andersson S., Lindler L., Titball R.,
RA Garcia E.;
RT "Complete genome sequence of Francisella tularensis LVS (Live Vaccine
RT Strain).";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AM233362; CAJ80183.1; -; Genomic_DNA.
DR RefSeq; WP_011457525.1; NZ_CP009694.1.
DR PDB; 6WMP; EM; 2.98 A; C=1-1358.
DR PDB; 6WMR; EM; 3.46 A; C=1-1358.
DR PDB; 6WMT; EM; 4.43 A; C=1-1358.
DR PDBsum; 6WMP; -.
DR PDBsum; 6WMR; -.
DR PDBsum; 6WMT; -.
DR AlphaFoldDB; Q2A1M7; -.
DR SMR; Q2A1M7; -.
DR KEGG; ftl:FTL_1744; -.
DR OMA; FMTWEGY; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Transcription; Transferase.
FT CHAIN 1..1358
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300315"
FT HELIX 6..8
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 27..34
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 35..39
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 43..46
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 53..57
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 65..68
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 72..78
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 85..91
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 96..104
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 118..120
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 124..133
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 137..139
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 141..145
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 147..150
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 152..156
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 158..161
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 167..170
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 173..177
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 179..181
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 184..187
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 195..197
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 206..209
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 210..212
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 217..220
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 350..353
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 361..372
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 380..382
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 383..388
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 394..396
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 400..402
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 403..410
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 426..439
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 448..450
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 453..457
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 459..481
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 487..489
FT /evidence="ECO:0007829|PDB:6WMR"
FT HELIX 499..511
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 513..517
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 523..529
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 533..535
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 543..545
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 555..557
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 558..560
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 570..573
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 590..598
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 601..610
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 611..613
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 614..616
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 628..630
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 635..649
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 650..652
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 660..664
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 666..668
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 671..673
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 674..676
FT /evidence="ECO:0007829|PDB:6WMR"
FT HELIX 679..688
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 702..704
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 708..713
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 714..716
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 717..720
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 725..730
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 732..737
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 741..743
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 744..746
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 751..754
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 758..760
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 762..764
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 784..787
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 791..796
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 799..801
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 804..807
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 810..816
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 818..820
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 823..826
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 827..830
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 832..843
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 862..864
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 865..867
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 869..873
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 884..886
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 889..891
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 899..904
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 906..909
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 914..917
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 928..936
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 939..941
FT /evidence="ECO:0007829|PDB:6WMR"
FT HELIX 947..977
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 978..982
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1026..1031
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1032..1045
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1049..1054
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1060..1072
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1079..1082
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1087..1093
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1096..1098
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 1101..1105
FT /evidence="ECO:0007829|PDB:6WMR"
FT STRAND 1109..1111
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1116..1119
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1124..1146
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1155..1163
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1164..1168
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1173..1175
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1178..1187
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1198..1200
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1204..1214
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1218..1222
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 1227..1229
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1237..1240
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1243..1248
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1251..1253
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1257..1260
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1265..1267
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1273..1276
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1285..1293
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1296..1301
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 1302..1308
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1310..1322
FT /evidence="ECO:0007829|PDB:6WMP"
FT HELIX 1333..1342
FT /evidence="ECO:0007829|PDB:6WMP"
FT TURN 1343..1346
FT /evidence="ECO:0007829|PDB:6WMP"
FT STRAND 1347..1352
FT /evidence="ECO:0007829|PDB:6WMP"
SQ SEQUENCE 1358 AA; 151336 MW; 35582DE9E4B8054A CRC64;
MSYSYAEKKR IRKEFGVLPH ILDVPYLLSI QTESYKKFLT VDAAKGRLHS GLEIVLKQSF
PVESKNGQYE LHYVDYQIGE PTFDETECQV RGATYDAPLN VKLRLVVYNK DALPNEKIVE
DIREEYVYMG DIPLMTTNGT FIINGTERVV VSQLHRSPGA FFSKDDSEEG AFSARIIPYR
GSWLDFEFDS KGIIWARIDR KRKFCATVIL KALGYTQEQI LENFSESKTI TFNSKGFALR
LDNLSNMKGE LLKFDIVDAQ DNVIVKKNKK LTSRDVKKIK DAGVDSVAID FDLVSTLRVA
KDIVNEATGE VIAYANDDVT ESLLKSCVEV GMLELEVIDF ITTERGRYIS DTLKYDLTRN
TDEALVEIYK VLRPGDPPAA ASVKALFEGL FFIESRYSLS DIGRMKLNAR LGSDKVSKDI
YTLENSDIVG VIEELINIRD GKGKVDDIDH LGNRRVRSVG EMVENQFRIG LYRVEKGIRE
SMSLVHKDKL MPKDIVNSKP ITAAIKEFFT SGALSQFMDQ DNPLSEVTHK RRISALGPGG
LSRDRAGFEV RDVHATHYGR LCPIETPEGP NIGLINSLAS YARVNDYGFL EAPYRKVVDG
KVTDEIEYLS AIDEDNYVIA QASTKLDENN HFVEDIIQCR SGGEAIFTES SRVQYMDVSA
KQMVSAAAAL IPFLEHDDAN RVLMGANMQR QAVPTLKSEK PLVGTGMEKI VARDSGNCII
ARNVGEVAEV DSNRIVIKVD TEKSQTSNLV DIYSLTKFKR SNKNTCINQR PIVNVGDKVE
AGDILADGFA TDFGELSLGH NLMVAFMPWN GYNFEDSILL SERIVKDDKY TSIHIEEFTC
VARDTKLGPE EITADIPNVS ESSLAKLDES GIVHIGANVE AGDILVAKIT PKAEQQLTPE
ERLLRAIFNE KASNVVDSSL RMPSGTSGTV INVQVFENDK GGKSKRALKI EKELIDKARK
DFDEEFAVIE SVVKSSIEQE VVGAKIQKAK GLKKGAILTK EFLATLPLSK WLEISFEDEK
LEEKVQNARE YYEEAKIAID AKFEAKKKSI TQSNELSPGV LKTVKVFVAI KKRIQPGDKM
AGRHGNKGVV SRVLPVEDMP YMEDGTPVDV CLNPLGIPSR MNIGQILEAH LGLASYGLGK
KIEKTLEKTR KAAELRKTLE EVYNSVGDKK VNLEALNDEE ILTLCDNLKG GVPIATPVFD
GAKEEDIKSL LKIGGFATNG QMKLFDGRTG KPFDRHVTVG YMYMLKLDHL VDDKMHARST
GSYSLVTQQP LGGKAQFGGQ RFGEMEVWAL QAYGAAYTLR EMLTVKSDDI AGRSKMYKNI
VDGKLTMNVD VPESFNVLRN EVRALGIDMD FDYSSEEE
