RPOB_GEODF
ID RPOB_GEODF Reviewed; 1370 AA.
AC B9M6V2;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Geob_3632;
OS Geotalea daltonii (strain DSM 22248 / JCM 15807 / FRC-32) (Geobacter
OS daltonii).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC Geobacteraceae; Geotalea.
OX NCBI_TaxID=316067;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22248 / JCM 15807 / FRC-32;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C.,
RA Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA Kostka J., Richardson P.;
RT "Complete sequence of Geobacter sp. FRC-32.";
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP001390; ACM21973.1; -; Genomic_DNA.
DR RefSeq; WP_012648700.1; NC_011979.1.
DR AlphaFoldDB; B9M6V2; -.
DR SMR; B9M6V2; -.
DR STRING; 316067.Geob_3632; -.
DR EnsemblBacteria; ACM21973; ACM21973; Geob_3632.
DR KEGG; geo:Geob_3632; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_7; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000007721; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1370
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000165808"
SQ SEQUENCE 1370 AA; 153061 MW; 466C89F8FF31CFB1 CRC64;
MAYSIANNPL LRKNFAKIKK IIDIPNLIDI QKNSYKRFLQ LDVPAEARKY SGLEAVFKSV
FPIKDFSETA SLEYVSYSLG IPKYDVEECH QRGMTFAAPM KVKVRLVVWD VNKEPSTRSI
RDIKEQEVYF GEIPLMTENG TFIINGTERV IVSQLHRSPG VFYDHDKGKT HSSGKVLYSA
RVIPYRGSWL DFEFDHKDIL YVRIDRRRKM PATVLLKALG YSTDELLNFF YKSEEINFAG
EKLTKVADPE LLTNQKAAID IVDSATGEVL VKANRKFTKA AIRKMAEHGI KFIPITIEEL
VGKISSHDIV DPSTGEIVAE CNEELTQAKL EEIKSKGINT FKVLFIDNLH VTSSFRDTII
IDKIGSTDDA LIEIYRRLRP GDPPTLKSAL SLFENLFFNP ERYDLSAVGR LKLNYKLGLQ
VPLDCMTLTR EDVLEVVRYL IDLKNGRGNI DDIDHLGNRR VRAVGELLEN QYRIGLVRME
RAIKERMSLQ EVENLMPHDL INSKPVSAVV KEFFGSSQLS QFMDQTNPLS EVTHKRRLSA
LGPGGLTRER AGFEVRDVHP THYGRVCPIE TPEGPNIGLI ASLSTYARIN EHGFVETPYR
LVQEGKVTNE VRFFSALEEE GHAIAQANAE VDKDGRFVAD YISARKSGEF VLVGRDELEL
MDVAPMQLVS VAASLIPFLE NDDANRALMG SNMQRQAVPL LRADSPLVGT GMERVVARDS
GVSVVARHNG VVESVDASRI VVKIDEDEYD ETGTGVDIYN LIKFARSNQN TCINQRPVVK
IGDHVKRGDV IADGPSTDMG ELALGQNVLV AFMPWGGYNF EDSILISERL VKDDRYTSIH
IEEFECVARD TKLGKEEITA DIPNLGEETL KDLDESGIIR IGAEVRPGDI LIGKITPKGE
TQLSPEEKLL RAIFGEKAGD VRDTSLRVPP GVEGTVIGAK IFSRKGADKD ARTELIEQAE
EKKLRKDEQD EIRIIRESAV SKLKKLLVGK TAAVKVEGKD GKILIVKGKA ISEEALSSIP
IDRWDEISVA DDESTDDKVA QILSTLNQQI DIIKYVFDDK VQKLKRGDDL PPGVIKMVKV
YIAIKRKLQV GDKMAGRHGN KGVVSRILPE EDMPYLEDGR PVEIVLNPLG VPSRMNVGQI
LETHLGWAAK GIGWQIEEML EKNSEKTKIK TYLKEVYGNK EMNKFLDTLD DEELSNVAKR
LKRGVPMASP VFEGSSEEGI REMLGKAGFA HTAQVTLYDG KSGDAFKHKV TVGVMYVLKL
HHLVDDKIHA RSIGPYSLVT QQPLGGKAQF GGQRLGEMEV WAMEAYGAAY ALQEFLTVKS
DDVAGRTRMY EAIVKGKHTL EPGLPESFNV LIKELQSLGL DVELLENEED
