ID   RPOB_GEOSL              Reviewed;        1370 AA.
AC   Q748Y6;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=GSU2863;
OS   Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Geobacter.
OX   NCBI_TaxID=243231;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51573 / DSM 12127 / PCA;
RX   PubMed=14671304; DOI=10.1126/science.1088727;
RA   Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C.,
RA   Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J.,
RA   Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S.,
RA   Gwinn M.L., Kolonay J.F., Sullivan S.A., Haft D.H., Selengut J.,
RA   Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A.,
RA   Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E.,
RA   Lovley D.R., Fraser C.M.;
RT   "Genome of Geobacter sulfurreducens: metal reduction in subsurface
RT   environments.";
RL   Science 302:1967-1969(2003).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE017180; AAR36255.1; -; Genomic_DNA.
DR   RefSeq; NP_953905.1; NC_002939.5.
DR   RefSeq; WP_010943493.1; NC_002939.5.
DR   AlphaFoldDB; Q748Y6; -.
DR   SMR; Q748Y6; -.
DR   STRING; 243231.GSU2863; -.
DR   EnsemblBacteria; AAR36255; AAR36255; GSU2863.
DR   KEGG; gsu:GSU2863; -.
DR   PATRIC; fig|243231.5.peg.2891; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_7; -.
DR   InParanoid; Q748Y6; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000000577; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1370
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224060"
SQ   SEQUENCE   1370 AA;  152508 MW;  1681ACEB8F5B7194 CRC64;
     MAYSIANNQL LRKNFAKIKK IIDIPNLIDI QKNSYKRFLQ IDTPPEARKN SGLEAVFKSV
     FPIKDFSDTA SLEYVTYTLG APKYDVEECH QRGMTFAAPM KVKVRLVVWD VNKDTGVRSI
     RDIKEQEVYF GEIPLMTENG TFIINGTERV IVSQLHRSPG VFYDHDKGKT HSSGKVLYSA
     RVIPYRGSWL DFEFDHKDIL FVRIDRRRKM PATVLLKALG YSAEALLNYF YKSEEILVAA
     EGMWKKADPE LLASQKASVD IVAPKTGEVL LKANRKFTKA AIRKMTEHGI TQIPITMEEV
     LGKYASNDIV DPSTGEVIVE CNDEITQARL DDIKAKGITE FKVLFIDNLH VTSSLRDTLI
     ADKIAATEEA LIEIYRRLRP GDPPTLKSAQ ALFENLFFNA ERYDLSAVGR LKLNFKLGLN
     VPLDCQTLTK DDVLEVVRYL IDLKNGKGTI DDIDHLGNRR VRAVGELLEN QYRIGLVRME
     RAIKERMSLQ EVENLMPHDL INSKPVSAVV KEFFGSSQLS QFMDQTNPLS EVTHKRRLSA
     LGPGGLTRER AGFEVRDVHP THYGRVCPIE TPEGPNIGLI ASLSTYARIN EHGFVETPYR
     IVQDGKVTSD VKFFSALEEE GHAIAQANAE MDADGRFVNE YVSARKSGEF LLVHRDELEL
     MDVAPMQLVS VAASLIPFLE NDDANRALMG SNMQRQAVPL LKADSPLVGT GMERVVAKDS
     GVSVVARHTG IVESVDASRI VVKIDEDEFD ETGTGVDIYN LIKFARSNQN TCINQRPVVK
     VGDHVKRGDV IADGPSTDMG ELALGQNVVV AFMPWGGYNF EDSILVSEKL VKDDRYTSIH
     IEEFECVARD TKLGKEEITS DIPNLGEEAL KDLDESGIIR IGAEVKPGDI LVGKITPKGE
     TQLSPEEKLL RAIFGEKAGD VRDTSLRVPP GVEGTVIGAK IFSRKGADKD SRTEMIERME
     EDKLRKDEQD EIRIIRNSAV GKLKKLLVGK SAAVKVEDKS GRTVIAKGAA ITEEALDAIP
     LDRWDEISVS GEAGVDEKVS AILQTLQQQI DIIRYVFDDK VQKLKRGDDL PPGVIKMVKV
     YIAIKRKLQV GDKMAGRHGN KGVVSRILPE EDMPYMEDGR PVEIVLNPLG VPSRMNVGQI
     LETHLGWAAK GIGWKIEEML ESYSPADHIK GYLKDVYGTT EMDTFLDSLD KDELMNVAKR
     LQRGVAMASP VFEGASEEQI RSMLDKAGFD ETAQVSLYDG KSGEPFKHKV TVGVMYVLKL
     HHLVDDKIHA RSIGPYSLVT QQPLGGKAQF GGQRLGEMEV WAMEAYGAAY ALQEFLTVKS
     DDVAGRTRMY EAIVKGKHTL EPGLPESFNV LIKELQSLCL DVELLEGDED