RPOB_GEOSM
ID RPOB_GEOSM Reviewed; 1371 AA.
AC C6E4R4;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-SEP-2009, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=GM21_3335;
OS Geobacter sp. (strain M21).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC Geobacteraceae; Geobacter; unclassified Geobacter.
OX NCBI_TaxID=443144;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M21;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C.,
RA Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA Lovley D.;
RT "Complete sequence of Geobacter sp. M21.";
RL Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP001661; ACT19360.1; -; Genomic_DNA.
DR RefSeq; WP_015838554.1; NC_012918.1.
DR AlphaFoldDB; C6E4R4; -.
DR SMR; C6E4R4; -.
DR STRING; 443144.GM21_3335; -.
DR EnsemblBacteria; ACT19360; ACT19360; GM21_3335.
DR KEGG; gem:GM21_3335; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_7; -.
DR OMA; FMTWEGY; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1371
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000214479"
SQ SEQUENCE 1371 AA; 152900 MW; 01C784EB1C415EE3 CRC64;
MAYSIANNPL LRKNFAKIHK IIDIPNLIDI QKNSYKRFLQ LDTPVDARKN SGLEAVFRSV
FPIRDFSDTA SLEYVSYSLG APKYDVEECH QRGMTFAAPM KVKVRLVVWD VAKDPGTRSI
RDIKEQEVYF GEIPLMTDNG TFIINGTERV IVSQLHRSPG VFYDHDKGKT HSSGKVLYSA
RVIPYRGSWL DFEFDHKDIL YVRIDRRRKM PATVLLKALG YSNDALINYF YKSEDVKVGD
NGSMTKIADA ELLSNQKATA DIVDPATGEV ILKANRKFTK AAIRKMSEHG IKEIPISEEE
VVGKVASHDI YDPATGEIIV ECNEELTQAK LEEIIQKGIT TFQVLFIDNL HVTSSFRDTI
LIDKIGSTDE ALIEIYRRLR PGDPPTLKSA LVLFENLFFN AERYDLSAVG RLKLNYKLGV
DVPLDCMTLT REDILEVVRY LIELKNGKGN IDDIDHLGNR RVRAVGELLE NQYRIGLVRM
ERAIKERMSL QEVENLMPHD LINSKPVSAV VKEFFGSSQL SQFMDQTNPL SEVTHKRRLS
ALGPGGLTRE RAGFEVRDVH PTHYGRVCPI ETPEGPNIGL IASLSTYARI NEHGFVETPY
RVVKEGRVTD EVRFFSALEE EGHAIAQANA EIDETGRFAA DYISARKSGE FVLVGRDELE
LMDVAPMQLV SVAASLIPFL ENDDANRALM GSNMQRQAVP LLRADSPLVG TGMERVVARD
SGVSLVARHN GVVESVDASR IVVKIDEDQY DATGTGVDIY NLIKFARSNQ NTCINQRPLV
KVGDHVTAGD IIADGPSTDM GELALGQNVL IAFMPWGGYN YEDSILISER LVKDDRYTSI
HIEEFEAVAR DTKLGKEEIT SDIPNLGEET LKDLDESGII RIGAEVRPGD ILVGKITPKG
ETQLSPEEKL LRAIFGEKAG DVRDTSLRVP PGVEGTVIGA KIFSRKGADK DARTEIIEKA
EEMRLRKDEQ DEIRIIRDSA IGKLKKLLVG KTAAVKIEGT DGKVLIPKGA AITEEMLKSF
SMDRWDEISI ADDDTVDEKV AQTLSTLNQQ IDIIKYVFDD KVQKLRRGDD LPPGVIKMVK
VYIAIKRKLQ VGDKMAGRHG NKGVVSRILP EEDMPYMEDG RPVEIVLNPL GVPSRMNVGQ
ILEMHLGWAA KGLGWKIEEF LDKNAPHDEI KRFLKGAYNN PDMDRFLDKL EGEELLNVAK
RLKRGVPMSS PVFEGASEES IQSMLSHAGF STTGQVTLFD GKSGDKFMHQ VTVGIMYFLK
LHHLVDDKIH ARSIGPYSLV TQQPLGGKAQ FGGQRLGEME VWAMEAYGAA YALQEFLTVK
SDDVAGRTRM YEAIVKGKHT LEPGLPESFN VLIKELQSLG LDVELLEGDE D
