RPOB_GEOTN
ID RPOB_GEOTN Reviewed; 1190 AA.
AC A4IJI1;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=GTNG_0098;
OS Geobacillus thermodenitrificans (strain NG80-2).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=420246;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NG80-2;
RX PubMed=17372208; DOI=10.1073/pnas.0609650104;
RA Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X.,
RA Han W., Peng X., Liu R., Wang L.;
RT "Genome and proteome of long-chain alkane degrading Geobacillus
RT thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000557; ABO65485.1; -; Genomic_DNA.
DR RefSeq; WP_008881953.1; NC_009328.1.
DR AlphaFoldDB; A4IJI1; -.
DR SMR; A4IJI1; -.
DR STRING; 420246.GTNG_0098; -.
DR EnsemblBacteria; ABO65485; ABO65485; GTNG_0098.
DR KEGG; gtn:GTNG_0098; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_9; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000001578; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1190
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300321"
SQ SEQUENCE 1190 AA; 133412 MW; 2940B9D60B71AB59 CRC64;
MTGRLVQYGR HRQRRSYARI SEVLELPNLI EIQTSSYQWF LDEGLREMFK EISPIEDFSG
NLSLEFIDYS LGEPKYSVEE AKERDVTYAA PLRVKVRLIN KETGEVKEQD VFMGDFPLMT
ETGTFIINGA ERVIVSQLVR SPSVYYSDKV DKNGKRGYSA TVIPNRGAWL EYETDAKDVV
YVRIDRTRKL PVTVLLRALG FSSDQEIIDL LGDNEYLRNT LEKDNTDSTE KALIEIYERL
RPGEPPTLEN AKSLLASRFF DPKRYDLASV GRYKINKKLH IKNRLFNQRL AETIADPETG
EIIAEAGTMI DRRTLNRLLP YLEKGAGLQT YRPTEGVADG QISVQTVKIY APNDPDNEKV
INIIGNGFIA EDVKHITPAD IIASISYFFN LLHGVGDTDD IDHLGNRRLR SVGELLQNQF
RIGLSRMERV VRERMSIQDA NTITPQQLIN IRPVIAAIKE FFGSSQLSQF MDQTNPLAEL
THKRRLSALG PGGLTRERAG FEVRDVHYSH YGRMCPIETP EGPNIGLINS LSTYAKVNKF
GFIETPYRRV DPETGKVTDQ IDYLTADEED NYVVAQANVP LAEDGTFLEE NVIARFRGEN
IVVKRDRVDY MDVSPKQVVS AATACIPFLE NDDSNRALMG ANMQRQAVPL LQPEAPIVGT
GMEYVSAKDS GAAIICKHRG IVERVEAKEI WVRRLIEVDG KEVKGDLDKY RLLKFVRSNQ
GTCYNQRPIV KKGDIVEKGE ILADGPSMDK GELALGRNVL VAFMTWDGYN YEDAIIMSER
LVKEDVYTSI HIEEYEAESR DTKLGPEEIT RDIPNVGEDA LKNLDERGIV RIGAEVKDGD
LLVGKVTPKG MTELTAEERL LHAIFGEKAR EVRDTSLRVP HGGGGIVLDV KVFNREDGDE
LPPGVNQLVR VYIVQKRKIS EGDKMAGRHG NKGVISRILP EEDMPFLPDG TPIDIMLNPL
GVPSRMNIGQ VFELHLGMAA KKLGLHIASP VFDGATEEDV WNILEEAGMA RDAKTVLYDG
RTGEPFDNRV SVGIMYMIKL AHMVDDKLHA RSTGPYSLVT QQPLGGKAQF GGQRFGEMEV
WALEAYGAAY TLQEILTVKS DDVVGRVKTY EAIVKGENIP EPGVPESFKV LIKELQSLGM
DVTILTSDEQ EINMENFDDD DDHAPDAIMV DVKPVESEEA DEEKNAVTKE
