RPOB_GNEPA
ID RPOB_GNEPA Reviewed; 1108 AA.
AC A6BM50; B7ZIB8;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Gnetum parvifolium (Small-leaved jointfir) (Gnetum scandens var.
OS parvifolium).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Gnetopsida; Gnetidae; Gnetales; Gnetaceae; Gnetum.
OX NCBI_TaxID=33153;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17383970; DOI=10.1093/molbev/msm059;
RA Wu C.-S., Wang Y.-N., Liu S.-M., Chaw S.-M.;
RT "Chloroplast genome (cpDNA) of Cycas taitungensis and 56 cp protein-coding
RT genes of Gnetum parvifolium: insights into cpDNA evolution and phylogeny of
RT extant seed plants.";
RL Mol. Biol. Evol. 24:1366-1379(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=19166950; DOI=10.1016/j.ympev.2008.12.026;
RA Wu C.-S., Lai Y.-T., Lin C.-P., Wang Y.-N., Chaw S.-M.;
RT "Evolution of reduced and compact chloroplast genomes (cpDNAs) in
RT gnetophytes: Selection toward a lower-cost strategy.";
RL Mol. Phylogenet. Evol. 52:115-124(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AB295946; BAF64895.1; -; Genomic_DNA.
DR EMBL; AP009569; BAH11298.1; -; Genomic_DNA.
DR RefSeq; YP_002519787.1; NC_011942.1.
DR AlphaFoldDB; A6BM50; -.
DR SMR; A6BM50; -.
DR PRIDE; A6BM50; -.
DR GeneID; 7368180; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 4.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1108
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300442"
SQ SEQUENCE 1108 AA; 126744 MW; A7DAEFC6BC77A767 CRC64;
MQFDEKKEMF TIPEFGQIQL EGFCRFIEYD LLDKFVKFPK IANTQKEVEF LFDKNYKIIE
PSIKEKDAVY QRLTFSSKLF VPAFFIYWNK IKKKKIIYLG EIPLMNNNGT FLINGNYRVV
VNQLIRSPGI YYSLERKRTG NIYTSTLISD CGGRLKFEID IKQNIWIRIS RKKNVSILVF
FFAMGLDIEE ILKNTYYIKG FEGWGLICNE KLKHKLSRKK GAIFTFYEEL GSRGDNNDFV
FSESLSESLY KKFTNFLSKR CKLGRIGRRN LNKKLNLEIP DNEIFLLPQD VLAIIDYLIK
VSYGVGTVDN IDHLQNRRFF SVADLLKKEV GLALNRVKVL IQKTMQTIEM LRKKQNKRIM
LPEIPLVSTQ ITKTLKHFFG LHPLSQFLEQ TNSLAEILHA RKVSFLGPGG LTERTANFRA
RDIHPSYYGR FCPINTPEGQ NAGLIASLAI SARINFGFLE SPFYNVAKKY QKAKKIVYLS
PSEDVYYRIA LGNCLSVDQK IPEKKNTPTQ YHQEFLSIAW EQIHFRCFLP LQYFSIGVSL
IPFLEHNDAT RALMGSNMQR QAVPSVQPEK CIVGTGLEGQ VALDSGALAI STQEGRIQYS
DAATIVSVLK GNTTQTELQI YQRSNSNTLM HQKTHASQAK YVRKGQILAD GAAMLGGEIC
LGKNILVAYM PWQGYNFEDA ILISECLIYK DIFTSFHITR YETTICTAEC EKMTREIPRL
ATYSLRHLDK NGLVRVGSWV QPGDVLVGKL RPRSSEDFSR FPELRLLQDL FCTSPIKETC
LRANGKGRVI DVNWSKLQAF CKDDLEKGHQ DDDTEDIYDE AEDALEKVYQ LNNYNLSSDS
EKVHVYLLEK RKIQVGDKVA GRHGNKGIVS IVLSRQDMPF LQSGISLDMV LNPLSVPSRM
NVGQIFECLL GLAGTMNKHY RIPPFDERYE QEASRKLVFY ELYKASEQTA NPWIFELEHL
GKTQIFDGRT GEIFEQPVTT GNAYILKLIH QVNDKMHARS TGNYARITQQ PVQGKSKGGG
QRLGEMEVWA LESFGVAYVL REMLTVKADH IRARKKILRS ILDGHSVPKA DSATESFRVL
SKELNSLALE LNHTIISGKY FNLDRIEV
