RPOB_GRABC
ID RPOB_GRABC Reviewed; 1391 AA.
AC Q0BUQ6;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN OrderedLocusNames=GbCGDNIH1_0548;
OS Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Granulibacter.
OX NCBI_TaxID=391165;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1260 / CGDNIH1;
RX PubMed=17827295; DOI=10.1128/jb.00793-07;
RA Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E.,
RA Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.;
RT "Genome sequence analysis of the emerging human pathogenic acetic acid
RT bacterium Granulibacter bethesdensis.";
RL J. Bacteriol. 189:8727-8736(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000394; ABI61446.1; -; Genomic_DNA.
DR RefSeq; WP_011631256.1; NC_008343.2.
DR AlphaFoldDB; Q0BUQ6; -.
DR SMR; Q0BUQ6; -.
DR STRING; 391165.GbCGDNIH1_0548; -.
DR PRIDE; Q0BUQ6; -.
DR EnsemblBacteria; ABI61446; ABI61446; GbCGDNIH1_0548.
DR KEGG; gbe:GbCGDNIH1_0548; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000001963; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1391
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300323"
SQ SEQUENCE 1391 AA; 154583 MW; 88DEF369268258E7 CRC64;
MNAITQRDSK SFTGRKRIRK SFARIGDVVP MPNLIDVQRA SYEAFLQMNV HPDNRTPTGL
QEVFRSVFPI DDFAGRGRLE FVNYELEEPK YDVEECIQRG MTFAAPLKVI LRLIVWDVDE
DTGSRSIRDI KEQPVYMGDM PLMTDNGTFI INGTERVIVS QMHRSPGVFF DHDKGKTHSS
GKFLFAARVI PYRGSWLDFE FDSKDLVYVR IDRRRKLPVT TLLYALEGQR TEAAIASGTD
PQDLPEIHGM TAEEILSYFY TQVVFTSTPK GWARPFDPEA FRGITLAEAL IDADTGTVVA
EAGTKLTARS ARKIAEQAKT VLVGRADLLG RYMAEDLVND QTGEIYAEAG EELTEARLAA
LETLGVTSLP TLAVDQTTGP WIRNTLAIDK NSTRDDALID IYRVMRPGEP PTPETAEALF
RGLFFDMDRY DLSSVGRVKM NMRLGVDAPD TVRTLRKEDI LRTVKILCDL KDGRGAIDDI
DNLGNRRVRS VGELMENQYR LGLLRMERAI RERMGSVDID TVMPHDLINA KPAAAAVREF
FGSSQLSQFM DQTNPLSEVT HKRRLSALGP GGLTRERAGF EVRDVHPTHY GRICPIETPE
GPNIGLINSL ATYAKVNKYG FIETPYRMVE DGKVSDQWRY LSAMEEDRLV VAQADAPRDS
TGKLTEELIS VRRQGDFRLV KPEDVTSMDV SPKQLVSVAA ALIPFLENDD ANRALMGSNM
QRQAVPLIRA DAPLVGTGME AAVARDSGAT IVARRAGVVD QIDGARIVVR ASGEDGATQG
VDIYRLRKFM RSNQSTCINQ RPLVKVGDHV ISGDIIADGP STELGELALG RNVLCAFMPW
NGYNFEDSIL ISERIARDDV FTSIHIEEFE VMARDTKLGQ EEITRDIPNV GEEALRNLDE
AGIVYIGAEV NPGDILVGKV TPKGESPMTP EEKLLRAIFG EKASDVRDTS LKLPPGTTGT
IVDVRVFSRR GVDKDERAMA IERAEIERLA KDRDDERGIQ ERAFLNRLRE KLMGHPASGG
FKGIKAGTII TDEVLAEHPR GAWRNISVQD DAVVADIELL KREFDAAVAK IQARFEGKVE
KLQRGDELPP GVMKMVKVFV AVKRKLQPGD KMAGRHGNKG VVSRVVPVED MPFLEDGTPV
DLVLNPLGVP SRMNVGQILE THLGWACANL GRQIGELVED YRRNGQQRDE LLSRLREIYG
ENVYNDVIAD MNNDQLIELA ENLRKGVPIA TPVFDGARPS DIENMLSRAG LDTSGQVVLT
DGRTGEPFER KVTVGYIYML KLHHLVDDKI HARSIGPYSL VTQQPLGGKA QFGGQRFGEM
EVWALEAYGA AYTLQEMLTV KSDDVSGRTK VYEAIVRDQD SFEAGIPESF NVLMKELKSL
GLNVDLENRS A
