RPOB_HELMI
ID RPOB_HELMI Reviewed; 1154 AA.
AC B0TC48;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Helmi_13220;
GN ORFNames=HM1_1370;
OS Heliobacterium modesticaldum (strain ATCC 51547 / Ice1).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Heliobacteriaceae;
OC Heliomicrobium.
OX NCBI_TaxID=498761;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51547 / Ice1;
RX PubMed=18441057; DOI=10.1128/jb.00299-08;
RA Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M.,
RA Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E.,
RA Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T.,
RA Raymond J., Chen M., Blankenship R.E., Touchman J.W.;
RT "The genome of Heliobacterium modesticaldum, a phototrophic representative
RT of the Firmicutes containing the simplest photosynthetic apparatus.";
RL J. Bacteriol. 190:4687-4696(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000930; ABZ83947.1; -; Genomic_DNA.
DR RefSeq; WP_012282463.1; NC_010337.2.
DR AlphaFoldDB; B0TC48; -.
DR SMR; B0TC48; -.
DR STRING; 498761.HM1_1370; -.
DR PRIDE; B0TC48; -.
DR EnsemblBacteria; ABZ83947; ABZ83947; HM1_1370.
DR KEGG; hmo:HM1_1370; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_9; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000008550; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1154
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000141701"
FT REGION 1108..1136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1108..1126
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1154 AA; 129582 MW; 331E991C224A58AB CRC64;
MVYPVQCGPR ERWTFARIQE VMDMPNLIEI QQNSYRWFLE EGLREMFRDI SPIQDFTGNL
ILEFIDYALG EPKYSVEECK ERDVTFAAPL RVKVRLINKE TGEVKEQEVF MGDFPLMTDK
GTFIINGAER VIVSQLVRSP GVYYNEAIDP PSGKKLFGAT IIPNRGAWLE FETDINDNIF
VRIDRTRKLP ATVLIRALGY SNNAQVQELF DGDERIRITL ERDNTENTEE ALVEIYKRLR
PGEPPTVDSA RSLLETLFFD PKRYDLAKVG RYKINKRLGI SVEREVRHLT KDDIIAAIRE
LLKLMQGDGR ADDIDHLGNR RLRSVGELLQ NQFRIGLSRM ERVVRERMTI QDVEVITPQV
LINIRPVVAA IKEFFGSSQL SQFMDQTNPL AELTHKRRLS ALGPGGLSRE RAGFEVRDVH
HSHYGRMCPI ETPEGPNIGL IGSLSTFARI NEYGFIETPY RKVDKERGVV TDQIDYLTAD
EEDKYIVAQA NAPLDEEGRF KEKRVNARHG HDILVVPVEK VDYMDVSPKQ VVSIATALIP
FLEHDDANRA LMGANMQRQA VPLLRTDAPY VGTGMEFKAA YDSGVCAIAR KAGYVERVTG
DEILIRCDDG TTEHHKLLKF LRSNQGTCIN QKPICHKGQR IEAGQIIADG PSTDQGELAL
GRNVLVAFMT WEGYNYEDAI LVNEKLVKED YFTSIHIEEY ECDSRDTKLG PEEITRDIPN
VGEEVLKDLD DRGIIRVGAE VRPGDILVGK VTPKGETELT AEERLLRAIF GEKAREVRDT
SLRVPHGEAG KIVDVKVFSR ENGDELAPGV NQLVRVYIAQ KRKISEGDKM AGRHGNKGVI
SRIMPEADMP YLPDGTPVEI VLNPLGVPSR MNIGQILETH LGWASKHMDR GVLIEGKPSG
EKGIRVATPV FDGATEDDIF EFLTKAGLPK ANEIALTDTR TESLRESMAA VPLGKSILYD
GRTGEPFDNP ITVGYMYMLK LAHLVDDKIH ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME
VWALEAYGAA YTLQEILTVK SDDVVGRVKT YEAIVKGENI PEPGVPESFK VLIKELQSLG
LDVKILSEDE REIEIKEVEE DVAETAKELG IDIQGEDRSE RAGEPASPDE MDDEEEIDYA
AGFLQDALEE LEED
