ID   RPOB_HELSJ              Reviewed;        1247 AA.
AC   Q2EEX2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta;
DE            Short=RNA polymerase subunit beta;
GN   Name=rpoB;
OS   Helicosporidium sp. subsp. Simulium jonesii (Green alga).
OG   Plastid; Non-photosynthetic plastid.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC   Chlorellales; Chlorellaceae; Helicosporidium; unclassified Helicosporidium.
OX   NCBI_TaxID=145475;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16630350; DOI=10.1186/1741-7007-4-12;
RA   de Koning A.P., Keeling P.J.;
RT   "The complete plastid genome sequence of the parasitic green alga,
RT   Helicosporidium sp. is highly reduced and structured.";
RL   BMC Biol. 4:12-12(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; DQ398104; ABD33970.1; -; Genomic_DNA.
DR   RefSeq; YP_635922.1; NC_008100.1.
DR   AlphaFoldDB; Q2EEX2; -.
DR   PRIDE; Q2EEX2; -.
DR   GeneID; 4100442; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 2.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1247
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300443"
SQ   SEQUENCE   1247 AA;  145911 MW;  BB5AFDD07F19D114 CRC64;
     MLLNDQKFLP INIHYLNQGR FDLNKLKNFN LLINQRENWR FFLINGLKLA FKEFEHFKIK
     NNNFIIDIVF QEKFLFFKQI RQAYLKKVET FGYNIFIPII ITQKILNKKN NLNLTFFKWL
     DLGILPSLSP KTSFYIDGIS RAVSTQKKKS PGLMFDFDLT KKSSLEKREG EAFLKIIAPR
     GSWFKISYSE DHIIFIEIRN IAYKIPIFTF LCALGFSLES IFQFFNYNIN NFKLIPNKYD
     NTNALIKARN DIFILYSDLL PLLNKTKKNF EIKYIINHYF FSYIWNSNTR YCGEATRLHL
     YNKVGSPLSL NSLFLEEIDF IYITKIFIYR LINNNFKDEN NDNLKNMRFR NGGDYIYLQT
     RQGLFDFESF LNINLHNIEN NSFFCNITTN KKKEDIYSIK KKKNIKNFNL SKISFLKHIL
     LKSWKSFFLS GTLSQFAENL NPLSYITHAR RFTSLGPGSL KKAEASIEVR SINPSYNGRV
     CPIETPEGFN AGLVHSFSLY SFKGVNGELL LPFYFIYKNI KQSYYLPPIF FNIEEESNQS
     IITGDLIHEK WNDFLNKKLS LRNNFHLDKN DISKISFQSL SPYHMISLAT SLIPFLEHND
     ANRALMGSNM QRQTVPVLGA HNFIVRTGLD TKFFSDISSI PISQINGFNF ESNNNYCYFY
     KIDNLITLNN ISYNYIKKTN HNSLYINESI KKKNPWHQQG DFLINNSVIE NGKLALGPNL
     LLAYLPWHGY NYEDAVIINE RCVSQKILTS LHIITLTNSL ELAYREINKK KEFFYEVPVK
     LEILFSFFAP FYYNLKYYKN QEFSQFEKKN PSYYTNNINI GTFLKKGDCI FSKCRIRFSF
     SRELRLQYKE LFILLREAFP TFEDFKNFAN IFAYDKYKQK LDRKKQKKNT RLKTDNFIFN
     KNSTLFKKHK LKALINYYNI EDSTIYAQKN QEGLIISKHL KKVPMTYTSI TPFDLEEFLS
     TKESKKKEYY NLFFTKSINI EILQLRDIQL GDKCAGRHGN KGIISKICPI NEMPLLPDGT
     PIDIILNPLG IPSRMNVGQI FESLLGLAGF YLNETYTINI FDEQFGLEAS RSFVLSKLYK
     ASILSNKPWL FLKKNAGKTN IIDGLTGKYF QQPITIGYSS ILKLIHMVTD KLHGRSIGGY
     STLTLQPTKG KSLLGGQRVG EMELWALQGY GASWALQELY TLKSDLVEAR QHFQNYINSF
     NIESNIYDRN DISLTELESL IISDTFKPYT LECFLEDLKA LCIYIDY