RPOB_HORVU
ID RPOB_HORVU Reviewed; 1076 AA.
AC A1E9I1;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Hordeum vulgare (Barley).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Morex;
RX PubMed=17534593; DOI=10.1007/s00122-007-0567-4;
RA Saski C., Lee S.-B., Fjellheim S., Guda C., Jansen R.K., Luo H.,
RA Tomkins J., Rognli O.A., Daniell H., Clarke J.L.;
RT "Complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor
RT and Agrostis stolonifera, and comparative analyses with other grass
RT genomes.";
RL Theor. Appl. Genet. 115:571-590(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; EF115541; ABK79403.1; -; Genomic_DNA.
DR RefSeq; YP_874643.1; NC_008590.1.
DR AlphaFoldDB; A1E9I1; -.
DR SMR; A1E9I1; -.
DR GeneID; 4525093; -.
DR OMA; FMTWEGY; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1076
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300444"
SQ SEQUENCE 1076 AA; 121653 MW; 438CC3DBBF2C34EE CRC64;
MLRNGNEGMS TIPGFSQIQF EGFFRFINQA LAEELDKFPT IKDPDHEIAF QLFAKGYQLL
EPSIKERDAV YESLTYSSEL YVSARLIFGF DVQKQTISIG NIPIMNSLGT FIINGIYRIV
INQILLSPGI YYRSELDHKG ISIYTGTIIS DWGGRSELAI DKKERIWARV SRKQKISILV
LSSAMGSNLR EILDNVSYPE IFLSFPNAKE KKRIESKEKA ILEFYQQFAC VGGDLVFSES
LCEELQKKFF QQKCELGRIG RRNMNRRLNL DIPQNNTFLL PRDVLAATDH LIGMKFGTGI
LDDDDMNHLK NKRIRSVADL LQDQFGLALG RLQHAVQKTI RRVFIRQSKP TPQTLVTPTS
TSILLITTYE TFFGTYPLSQ VFDQTNPLTQ TVHGRKVSCL GPGGLTGRTA SFRSRDIHPS
HYGRICPIDT SEGINVGLTG SLAIHARIDH LWGSIESPFY EISAEKAKEK KERQVVYLSP
NRDEYYMIAA GNSLSLNQGI QEEQVVPARY RQEFLTIAWE QIHVRSIFPF QYFSIGGSLI
PFIEHNDANR ALMSSNMQRQ AVPLSRSEKC IVGTGLERQT ALDSRVSVIA EREGKIISTD
SHKILLSSSG KTISIPLVNH RRSNKNTCMH QKPRVPRGKS IKKGQILAEG AATVGGELAL
GKNVLVAYMP WEGYNFEDAV LISERLVYED IYTSFHIRKY EIQTDTTSQG SAEKITKEIP
HLEEHLLRNL DKNGVVRLGS WVETGDILVG KLTPQIASES SYIAEAGLLR AIFGLEVSTS
KETSLKLPIG GRGRVIDVKW IQRDPLDIMV RVYILQKREI KVGDKVAGRH GNKGIISKIL
PRQDMPYLQD GTPVDMVFNP LGVPSRMNVG QIFESSLGLA GDLLKKHYRI APFDERYEQE
ASRKLVFSEL YEASKETKNP WVFEPEYPGK SRIFDGRTGD PFEQPVLIGK SYILKLIHQV
DEKIHGRSTG PYSLVTQQPV RGRAKQGGQR VGEMEVWALE GFGVAHILQE ILTYKSDHLI
ARQEILNATI WGKRIPNHED PPESFRVLVR ELRSLALELN HFLVSEKNFQ VNREEV