ID   RPOB_HYPNA              Reviewed;        1378 AA.
AC   Q0BYA7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=HNE_2858;
OS   Hyphomonas neptunium (strain ATCC 15444).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomonadales;
OC   Hyphomonadaceae; Hyphomonas.
OX   NCBI_TaxID=228405;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15444;
RX   PubMed=16980487; DOI=10.1128/jb.00111-06;
RA   Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G.,
RA   Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D.,
RA   Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R.,
RA   Brinkac L.M., Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G.,
RA   Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N.,
RA   Ward N.L.;
RT   "Comparative genomic evidence for a close relationship between the
RT   dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter
RT   crescentus.";
RL   J. Bacteriol. 188:6841-6850(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000158; ABI77410.1; -; Genomic_DNA.
DR   RefSeq; WP_011647833.1; NC_008358.1.
DR   AlphaFoldDB; Q0BYA7; -.
DR   SMR; Q0BYA7; -.
DR   STRING; 228405.HNE_2858; -.
DR   PRIDE; Q0BYA7; -.
DR   EnsemblBacteria; ABI77410; ABI77410; HNE_2858.
DR   KEGG; hne:HNE_2858; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_5; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001959; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1378
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300327"
SQ   SEQUENCE   1378 AA;  153360 MW;  71DC217FE819C1FC CRC64;
     MALSFTEKKR IRKNFGRIPE AIEMPNLIEV QRESYEAFLQ MNTPREQRTD DGLGGVFKSV
     FPITDFSERA TLEYVSYEFE QPKFDVEECV QRDLTFQAPL KVRLQLVVFD VDEDTGARSV
     KEVKEQECYL GDIPLMTDKG TFVINGTERV IVSQMHRSPG VFFDHDKGKT HASGKLLFAA
     RIIPYRGSWL DFEFDAKDVL NIRIDRKRKL PATTMLYALG YDTEQILDQF YTRSIYRLDK
     KGWITSFRAD AWKGVKPEFE LIDAKTGKSV AEAGKKITAL KAKRMAEAGT DEILVTSEAL
     IGKFLARDVV NLETGEIFGE AGDALEEPII AEMREYGIDS IEVLDIDAGG RGPWLRNTLK
     ADKNETRFEA LSDIYRVMRP GEPPTQEAAD ALFGQLFFDP ERYDLSAVGR VKMNMRLSVA
     VREYESAADN MRTLRNEDII GVMKVILDLK DGKGEVDDID NLGNRRVRSV GELMENNYRI
     GLVRMERAIK ERMGAVDIDT VMPHDLINAK PVVAAVREFF GSSQLSQFMD QTNPLSEITH
     KRRLSALGPG GLTRERAGFE VRDVHPTHYG RICPIETPEG PNIGLINSLA THARVNKYGF
     IESPYRRVKD AKLTEEVVYL SAMEESIYSI AQANATVNDK GELVNEFVNA RVAGEATMIA
     KEEVQYMDVS PKQVVSVAAS LIPFLENDDA NRALMGSNMQ RQAVPLVKSE APFVGTGMEA
     VVARDSRAAI VARRAGVVEQ VDAMRIVVRA TEDLEGSKSG VDIYRLAKFR RSNQNSCINQ
     RPIAKVGDVV SKNDIIADGP STDLGELALG RNVLVAFMPW NGYNFEDSIL ISERIVRDDV
     FTSIHIEEFE VAARDTKLGP EEITRDIPNV GEEALRNLDE AGIVAVGAEV KAGDILVGKV
     TPKGESPMTP EEKLLRAIFG EKASDVRDTS LRVPPGDAGT VVDVRIFNRH GIDKDQRALQ
     IEREQIEKLQ EDKEDEQSIL ERNTYSRLRD ILIGKDAATG PKGFKAGKIA EAALEELTQR
     QWWEIELKSE KAQAELQALR EQFDASIREL EARFNDKVEK VQRGDDLPPG VMKVVKVFLA
     VKRKLQPGDK MAGRHGNKGV ISKINPLEDM PFLEDGTPVD IVLNPLGVPS RMNVGQILET
     HTGWACRGLG NIIDKALEEF HQKHDVAALK ASLTHAYGDK QEMPETDAEI IELAGNLRGG
     VPIATPVFDG AREEDINDLL TRAGLDTSGQ VRLFDGRTGV AFTRPVTVGY KYLLKLHHLV
     DEKIHARSTG PYSLVTQQPL GGKAQFGGQR FGEMEVWALE AYGAAYTLQE MLTVKSDDTA
     GRAKVYESIV RGDDSFEAGI PESFNVLIKE MRSLGLNVEL LEDRGGAIEE EEAPVAAE