ID   RPOB_IDILO              Reviewed;        1342 AA.
AC   Q5QWA5;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=IL0345;
OS   Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Idiomarinaceae; Idiomarina.
OX   NCBI_TaxID=283942;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-735 / DSM 15497 / L2-TR;
RX   PubMed=15596722; DOI=10.1073/pnas.0407638102;
RA   Hou S., Saw J.H., Lee K.S., Freitas T.A., Belisle C., Kawarabayasi Y.,
RA   Donachie S.P., Pikina A., Galperin M.Y., Koonin E.V., Makarova K.S.,
RA   Omelchenko M.V., Sorokin A., Wolf Y.I., Li Q.X., Keum Y.S., Campbell S.,
RA   Denery J., Aizawa S., Shibata S., Malahoff A., Alam M.;
RT   "Genome sequence of the deep-sea gamma-proteobacterium Idiomarina
RT   loihiensis reveals amino acid fermentation as a source of carbon and
RT   energy.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:18036-18041(2004).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE017340; AAV81188.1; -; Genomic_DNA.
DR   RefSeq; WP_011233607.1; NC_006512.1.
DR   AlphaFoldDB; Q5QWA5; -.
DR   SMR; Q5QWA5; -.
DR   STRING; 283942.IL0345; -.
DR   PRIDE; Q5QWA5; -.
DR   EnsemblBacteria; AAV81188; AAV81188; IL0345.
DR   KEGG; ilo:IL0345; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001171; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1342
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224063"
SQ   SEQUENCE   1342 AA;  150267 MW;  2A2789304E61EAB4 CRC64;
     MAYSYSEKKR IRKDFGKRPQ VLEVPYLLSM QLDSFKKFIE VDPEANNGLE AAFRSVFPIA
     SYSGTAELQY VSYHMGEPAF DVEECQIRGI TYSAPLRVKL RLVLFDKEAA AGTVKDIKEQ
     EVYMGEIPLM TENGTFVING TERVIVSQLH RSPGVFFDHD KGKSHSSGKV LYNARVIPYR
     GSWLDFEFDP KDNVFVRIDR RRKLPASIIL RALEYSTEEI LDMFFDTTYF EILKTKVKMQ
     LVPERLRGET AKFDIKGAEG EVLVEKGRRI TARHIRQLEQ LDIKEMEVPA EYMVGKVLAK
     DYANKKTGEV VGHANDVLTL ELIAELSEAG IKKIETLFVN DLDHGPYMSE TLRVDSSNNR
     LEALVEIYRM MRPGEPPTRE AAETLFDNLF FSEERYDLST VGRMKFNRRL GREDLSGDNT
     LSREDIVEVM KKLIEIRNGL DEVDDIDHLG NRRIRSVGEM AENQFRVGLV RVERAVKERL
     SLGDLDNTMP QDLINAKPIS AAVKEFFGSS QLSQFMDQNN PLSEVTHKRR ISALGPGGLT
     RERAGFEVRD VHPTHYGRLC PIETPEGPNI GLINSLSTFS RTNEYGFLET PYRRIENGSV
     TDDVDYLSAI EEGNYVIAQA NVGLNDKGEL TEDLVPCRHK NEFTLMSKEQ VQYMDVAPQQ
     IVSIAASMIP FLEHDDANRA LMGSNMQRQA VPTLRADKPL VGTGIERTIA VDSGVTVVAK
     RGGVVDYVDA SRIVVKVNEE EMVPGEAGID IYNLTKYTRS NQNTCINQKP TVNPGEPVTR
     GDVLADGPST DLGELALGQN MRVAFMPWNG YNFEDSILIS ERVAQEDRMT TIHIQELNCV
     ARDTKLGAEE ITSDIPNVGE SALGKLDESG VVYVGAEVNG GDILVGKVTP KGETQLTPEE
     KLLRAIFGEK ASDVKDSSLR VPNGVNGTVI DVQVFTRDGV EKDKRAVAIE EMQLAKVKKD
     LTEEFKILEE GIYNRARNLL LSNGFDEAKL ASVDRSSWLT QDLGNEDAQA QLEQIAEQYQ
     QIRDDFDTKF ETKRRKITQG DDLAPGVLKI VKVYLAVRRS IQPGDKLAGR HGNKGVISTI
     IPVEDMPHDD EGNPVDIVLN PLGVPSRMNI GQILETHLGM AARGIGSKIE KMLKQQRAIS
     ELRDFLQQVY DLGESRQKVD ITEFTDDEVM RLAGNLKAGL PTATPVFDGA VEEEIKALLK
     LADLPESGQI RLFDGRTGDK FERDVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG
     GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVNG RTKMYKNIVD NNLQMEAGMP
     ESFNVLLKEI RSLGINIELE QN