RPOB_ILLOL
ID RPOB_ILLOL Reviewed; 1070 AA.
AC A6MMT6;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Illicium oligandrum (Star anise).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Austrobaileyales; Schisandraceae; Illicium.
OX NCBI_TaxID=145286;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17644003; DOI=10.1016/j.ympev.2007.06.004;
RA Hansen D.R., Dastidar S.G., Cai Z., Penaflor C., Kuehl J.V., Boore J.L.,
RA Jansen R.K.;
RT "Phylogenetic and evolutionary implications of complete chloroplast genome
RT sequences of four early-diverging angiosperms: Buxus (Buxaceae),
RT Chloranthus (Chloranthaceae), Dioscorea (Dioscoreaceae), and Illicium
RT (Schisandraceae).";
RL Mol. Phylogenet. Evol. 45:547-563(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABQ52511.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; EF380354; ABQ52511.1; ALT_INIT; Genomic_DNA.
DR RefSeq; YP_001294262.1; NC_009600.1.
DR AlphaFoldDB; A6MMT6; -.
DR SMR; A6MMT6; -.
DR GeneID; 5236720; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1070
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300445"
SQ SEQUENCE 1070 AA; 120470 MW; 5761C5856216288A CRC64;
MLRDGNEGMS TIPGSSQIQF EGFCKFIDQG LAEELHKFPK MEDTDQEMEF QLFVETYQLV
EPLIKERDAV YESLTYSSEL YVSAGLIWRA GRDMRKQTVF IGNIPLMNSL GTSLVNGIYR
IVVNQILQSP GIYYRSELDH NGISVYTGTI ISDWGGRSEL EIDRKARIWA RVSRKQKISI
LVPSSAMGSN LRDILDNVCY PEIFLSFPND KEKKKIGSKE NAVLEFYQQF ACVGGDPVFS
ESLCKELQKK FFQQRCELGR IGRRNMNQRL NLDIPQNSTF LLPRDVLAAA DHLIGMKFGM
GTLDDLNHLK NKRIRSVADL LQDQFGLALV RLENVVRGTI CGAIRHKLIP TPHNLVTSTP
LTTTYESFFG LHPLSQVLDR TNPLTQIVHG RKWSYLGPGG LTGRTASFRI RDIHPSHYGR
ICPIDTSEGI NVGLIGSLAI HARIGDWGSI ESPFYEISER LKEEQMVYLS PRRDEYYMVA
AGNSLALNRG IQEEQVVPAR YRQEFLTIAW EQIHLRSIYP FQYFSIGASL IPFIEHNDAN
RALMSSNMQR QAVPLSHSEK CIVGTGLERQ AALDSGGSAI AEHEGKITYT DTEKIVLSGN
GDTISIPLVM YQRSNKNTCM HQKPQVHRGK CVKKGQILAD GAAIIGGELA LGKNILVAYM
PWEGYNSEDA VLISERLVYG DIYTSFHIRK YEIQTHVTSQ GPERITNEIP HLEAHLLRNL
DKNGIVMLGS WVETGDILVG KLTPQTAKES SYAPEDRLLR AILGIQVSTA KETCLKLPIG
GRGRVIDVRW IQKKRGSSSN PETIRVYISQ KREIKVGDKV AGRHGNKGII SKILPRQDMP
YLQDGTPVDM VFNPLGIPSR MNVGQMFECS IGLAGNLLNR HYRIIPFDER YEQEASRKLV
FPELYKASKQ TANPWVFEPE YPGKSRIFDG RTGDPFEQPV IIGKSYMMKL IHQVDDKIHG
RSSGHYALVT QQPLRGRAKH GGQRVGEMEV WALEGFGVAH ISQEMLTYKS DHIRARQEVL
GTTIIGGTIP NPDGAPESFR LLVRELRSLA LELNHFLVSE KSFQINRKEA
