RPOB_JANSC
ID RPOB_JANSC Reviewed; 1385 AA.
AC Q28UX7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Jann_0568;
OS Jannaschia sp. (strain CCS1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Jannaschia; unclassified Jannaschia.
OX NCBI_TaxID=290400;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCS1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D.,
RA Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J.,
RA Larimer F., Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W.,
RA Buchan A., Gonzalez J.M., Schell M.A., Richardson P.;
RT "Complete sequence of chromosome of Jannaschia sp. CCS1.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABD53485.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000264; ABD53485.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_011453694.1; NC_007802.1.
DR AlphaFoldDB; Q28UX7; -.
DR SMR; Q28UX7; -.
DR STRING; 290400.Jann_0568; -.
DR EnsemblBacteria; ABD53485; ABD53485; Jann_0568.
DR KEGG; jan:Jann_0568; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000008326; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1385
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300328"
SQ SEQUENCE 1385 AA; 154187 MW; E2AA7FFFAF28FB44 CRC64;
MPQTYAGQKR IRKFYGKIRE VLEMPNLIEV QKSSYDLFLK SGDQLEPMDG EGIKGVFQSV
FPIKDFNETA ILEFVKYELE TPKFDVEECQ QRDLTYAAPL KVTLRLIVFD IDEDTGAKSV
KDIKEQDVFM GDMPLMTPNG TFVVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAC
RIIPYRGSWL DFEFDAKDIV FSRIDRRRKL PVTTLLYALG LDQEGIMDAY YDTVTFKMVK
NKGWSTKFFP ERVRGTRPTT DLVDAKTGEV IAEAGKKVTP RAVKKWIDEG SIENLLVPFD
GIVGRFAAKD IINEETGAIY VEAGDELTWE LDKAGEVSGG TLKELIDAGI TEIPVLDIDN
VNVGPYMRNT LAVDKNLNRE SALMDIYRVM RPGEPPTVEA ASNLFDQLFF DSERYDLSAV
GRVKMNMRLD LDAEDTMRTL RKEDIISCIK ALVELRDGRG DIDDIDHLGN RRVRSVGELM
ENQYRVGLLR MERAIKERMS SVEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP
LSEVTHKRRL SALGPGGLTR ERAGFEVRDV HPTHYGRMCP IETPEGPNIG LINSLATYAR
VNKYGFIETP YRRVNDAVVS DDVVYMSATE EMRHTVAQAN ANLDDDGKFV NDMVNTRMSG
EYTLNPREAI DLIDVSPKQL VSVAASLIPF LENDDANRAL MGSNMQRQAV PLLQADAPFV
GTGIESVVAK DSGAAIMAKR GGVIDQVDAQ RIVIRATEDL ELGDAGVDIY RLRKFQRSNQ
NTCINQRPLV KVGDKIGKGE VIADGPSTDI GELALGKNVI VAFMPWNGYN YEDSILISER
ISRDDVFTSI HIEEFEVAAR DTKLGPEEIT RDIPNVGEEA LRNLDEAGIV YIGAEVEPGD
ILVGKITPKG ESPMTPEEKL LRAIFGEKAS DVRDTSLRVK PGDYGTIVEV RVFNRHGVEK
DERALQIERE EVERLARDRD DELVILERNI YARLRGMIMG KTAVKGPKGV KPNTVIDEDL
LDGQLSRGQW WQLALEDEKD AAHIEALNQQ FDTQKRALDH RFEDKVEKVR RGDDLPPGVM
KMVKVFIAVK RKLQPGDKMA GRHGNKGVIS KVVPMEDMPF LADGTPVDFV LNPLGVPSRM
NVGQILETHM GWAARGMGLQ IDEALDEYRR SGDMTPVRDA LKIAYGDDVY DDAFADRDEE
SLLEAAGNVT KGVPIATPVF DGAKEADVND ALIRAGFSTS GQSKLFDGRT GEQFAREVTV
GVKYLLKLHH LVDDKIHARS TGPYSLVTQQ PLGGKAQFGG QRFGEMEVWA LEAYGAAYTL
QEMLTVKSDD VAGRTKVYES IVKGEDNFEA GVPESFNVLV KEVRGLGLNM ELLDAEDDEG
GIAAE
