RPOB_KOSOT
ID RPOB_KOSOT Reviewed; 1173 AA.
AC C5CGD9;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Kole_1838;
OS Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1).
OC Bacteria; Thermotogae; Kosmotogales; Kosmotogaceae; Kosmotoga.
OX NCBI_TaxID=521045;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1733 / DSM 21960 / TBF 19.5.1;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C.,
RA Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA Noll K.;
RT "Complete sequence of Thermotogales bacterium TBF 19.5.1.";
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP001634; ACR80520.1; -; Genomic_DNA.
DR RefSeq; WP_015869163.1; NC_012785.1.
DR AlphaFoldDB; C5CGD9; -.
DR SMR; C5CGD9; -.
DR STRING; 521045.Kole_1838; -.
DR PRIDE; C5CGD9; -.
DR EnsemblBacteria; ACR80520; ACR80520; Kole_1838.
DR KEGG; kol:Kole_1838; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_0; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000002382; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1173
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000214481"
SQ SEQUENCE 1173 AA; 131840 MW; 2F37D8E3FB32D814 CRC64;
MRKAMFGKRE RWVFGKVIEP LEIPNLIEIQ LKSFKWFLEE GLLDVLKKYS PIKSQIQRPD
SRKNEKGFSL EFVRTRIGNP KYNIQECKDK GLTYSVPLYV TVRITDLYSG EIKEEEAFFG
YLPYMTDNAS FIINGAERVI VNQLVRSPGV YFVDEPTKAS SGSLPILVAH FLPVKGAWLE
ILLNPNKKVL QVRIDRRRKM NLFLLLKTLG YEDDLELLDL FVHDVDANDE YVLLEHVGSI
ILSDVKTRNE LIAERGAVLT EALAKKIAES DVDVIKLPMP IAMSTLKAFH RTYGENIKSE
DAYIEIFRKL RPGEIPRINA AKNYLHNLYF SPDRFDFSEV GRHKINSRLR KVYKEYLKEV
KKTEVPEDIE YAEESTVLTE LDIVLAARHL AQLESHPELL DTKDHLGNKR VRTVGELMQN
EFEKAFVKIH KLLEEKLTIY TSFDKVTVQN LVNVRTLMTT LHQFFATSQL SQFMDQVNPL
AELTHKRRLS AIGPGGLKRE HARFEVRDVH HSHYGRMCPI ETPEGANIGL MTSLSTYATI
DKYGFLLTPY RKVKNGKVTD EIVYLAADEE ELYNIAQSTI EVDDNGNIVE SVVEVRHAGE
IKYVEKEKVD LMAISPKQIV SVSTSLIPFL EHDDANRALM GSNMQRQAVP VIKPEAPIVG
TGVEAIAARD SGYVVLAKHR GIVKKVDSRK IIIERIDEDG NPILSESGEP IQDVYTLHKF
IRTNQDTTIN QRPIVSQGET VEKGDPIADG PSTDMGELAL GKNIFVAFLP WEGYNFEDAI
LVSQELLEEE TFTTIHIEMY ETVARDTQLG PEEITADIPN VSKELLKNLD ENGIVRVGAY
VSSGDILVGK VTPKGESDTT PEEKIIRSVF GDRGRDVKDT SLRVPHGVEG RVIDVKVFDK
EEISELGSGV NKLVKVYVAC RKPLDVGDKL AGRHGNKGVV SNIIPKEDMP FLPDGTPVQI
VLSPLGVPSR MNVGQVLETH LGWLGQLTNR YFATPIFDGA KEDEIMEELY KARKAAGIDV
GDSKKEKNGK VLLRDGRSGL PFDYPVVVGV MYMLKLVHIA KDKIHARSTG PYSLIHQQPL
GGKAHFGGQR FGEMEVWALE AHGAAHTLNE VLTVKSDDIK GRNEVYKAIL KGLNVPGPGI
PESFKVLIKE LQGLALDIRV YDQNGNELDV DKL
