ID   RPOB_LACDB              Reviewed;        1221 AA.
AC   Q04C22;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LBUL_0342;
OS   Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365 / Lb-18).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=321956;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-365 / Lb-18;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABJ58000.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000412; ABJ58000.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_035169457.1; NC_008529.1.
DR   AlphaFoldDB; Q04C22; -.
DR   SMR; Q04C22; -.
DR   GeneID; 66399380; -.
DR   KEGG; lbu:LBUL_0342; -.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   BioCyc; LDEL321956:LBUL_RS01600-MON; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1221
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300332"
FT   REGION          1176..1221
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1187..1212
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1221 AA;  135974 MW;  8A406A8CDECE01E5 CRC64;
     MLNGHVVNYG QHRTRRSFSR IKEILPLPNL TDVQTESYKW FLDEGVKEVF DDILPISDTS
     GRLTLEYVDY KLQEPKYTVD ESRKHDATYS APMHVTLKLT NHETGEIKTQ DVFFGDLPLM
     TKSGSFIVNG AERVIVSQLV RSPGVYYSGE FDKNGRQIFG TTVIPNRGAW LEFETDAKNI
     SYVRVDRTRK LPLSVLVRAL GFGSDSEIKE IFGDSDTLDL TLDKDVHKNP ADSRVAEALK
     DIYDRLRPGE PKTTDSSRSL LVSRFFDPRR YDLAAVGRYK VNKKLSLKNR LLGYTLAETL
     ADPDTGEVLA AKGTVVNNEV MDVLKDYLDR DDFKTVTYTP SDEGVIPEPV TVQEIKVFSR
     EIPDREIKLI SNGHIAEDVK CITPADIIAS VNYFLELQEG VGNIDDIDHL GNRRIRRVGE
     LLQNQMRIGL ARMERVVRER MSIQDAATVT PQQLINIRPI VGSIKEFFGS SQLSQFMDQN
     NPLGELTHKR RMSALGPGGL SRDRAGYEVR DVHYTHYGRL CPIETPEGPN IGLINSMATY
     AIINKYGFLE TPYRRVSWAT HKVTDKIDYL TADEEDNYII AGANTPLNED GSFVDDVILC
     RHREDNVEVS PDRIDYIDVI PKQVVSVTSA CIPFLENDDS NRALMGANHQ RQAVPLINPH
     GPLVATGMEY RAGHDSGDAL LAEADGEVEY VDANEIRVRR EDQTLDTYTL EKYRRSNATK
     NYNQTPNVKR GDKVVDGQVI ANGPSMADGE LALGQNPVIA FTTWNMYNFE DAIMLSERLV
     KEDVYTSIHI EDYDSEARDT KLGPEEITRE IPNVGEDALK DLDENGIIRI GAEVHDGDIL
     VGKVTPKGIT ELSAEERLLH AIFGEKAREV RDTSLRVPHG GGGVVQDVQV FTREAGDELA
     PGVNTLVRVY IVQKRKIQVG DKMSGRHGNK GTVALIAPVE DMPYLPDGTP VDICLNPMGV
     PSRMNIGQLL EIHLGRAARA LGIHVATPVF DGASEDDVWD FVREAGVDSD GKTVLYDGRT
     GEPFHNRVSV GVMYYLKLTH MVDDKIHARS IGPYSLVTQQ PLGGKAQFGG QRFGEMEVWA
     LEAYGAAYTL QEILTYKSDD VVGRVKAYEA IVKGERITKP GVPESFRVLV KELQSLGLDL
     RVLDSDENEV ELRDMDEDSN EHVNIDALSR LAEAQEKKKL AEEEAEIAAE AEAEGSAEED
     AAEADADANE AETADDDKAS K