RPOB_LACGA
ID RPOB_LACGA Reviewed; 1212 AA.
AC Q046D2;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LGAS_0284;
OS Lactobacillus gasseri (strain ATCC 33323 / DSM 20243 / BCRC 14619 / CIP
OS 102991 / JCM 1131 / KCTC 3163 / NCIMB 11718 / NCTC 13722 / AM63).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=324831;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33323 / DSM 20243 / BCRC 14619 / CIP 102991 / JCM 1131 / KCTC
RC 3163 / NCIMB 11718 / NCTC 13722 / AM63;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000413; ABJ59690.1; -; Genomic_DNA.
DR RefSeq; WP_011678765.1; NZ_WBMG01000001.1.
DR AlphaFoldDB; Q046D2; -.
DR SMR; Q046D2; -.
DR KEGG; lga:LGAS_0284; -.
DR HOGENOM; CLU_000524_4_1_9; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR BioCyc; LGAS324831:G1G6Y-282-MON; -.
DR Proteomes; UP000000664; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1212
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300333"
FT REGION 1176..1212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1176..1197
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1198..1212
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1212 AA; 136060 MW; 047C1034C182F72B CRC64;
MFSLLGHAVN YGSHRTRRSF SRIKEVLKLP NLTDVQTQSY KWFLNEGIRE MFDDIMPISD
FSGKLSLEFV DYKLLKPKYT LEEARDHDAN YSAPLHVTLK LTNHETGEIK TQDVFFGEFP
LMTDSGTFVI NGAERIIVSQ LVRSPGVYYH SDFDKNGRQI FGATVIPNRG AWLEYETDAK
DLAYVRIDRT RKLPLTVLIR ALGFGSDSEV ADMFGESDSL RFTLEKDIHK NPADSRVAEA
LKDIYERLRP GEPKTTDSSR SLLYARFFDP RRYDLAPVGR YKINKKLSLK NRLLRQTLAE
TLADPDTGEI IAKKGDVVTH EILDKLSPYL DRDDFKMVTY EPSKEGVLPD PVTVQEIKVY
SKVDPERVIK LMSNGHIADD VKHLTPADVL ASINYFFNLQ DNIGTTDDID HLGNRRIRRV
GELLQNQFRI GLARMERVVR ERMSIQDIST VTPQQLINIR PVVASVKEFF GSSQLSQFMD
QNNPLGELTH KRRMSALGPG GLSRDRAGYE VRDVHYTHYG RLCPIETPEG PNIGLINSLA
TYAIVNKYGF IETPYRRVSW DTHKVTDKID YLTADVEDNY IIAGANAPLN EDGSFKDKIV
LARHKEDNLE VTPDKIDYMD VIPKQVVSVT SACIPFLEND DSNRALMGAN HQRQAVPLIN
PHAPIVGTGM EYRAAHDSGD ALVAKAPGVV EYVDANEIRI RRDDDTLDKY VLEKFRRSNA
TKNYNQTPAV KQGERVVADE VIADGPAMEN GELALGQNPI IAFLTWNMYN YEDAVMISER
MVKDDVYTSI HIEDYESEAR DTKLGPEEIT REIPNVGEDA LKDLDEEGIV RIGAEVQDGD
ILVGKVTPKG VTELSAEERL LHAIFGEKAR EVRDTSLRVP HGGGGIVQNV QVFTREAGDE
LPPGVNKMVR VYIVQKRKIQ VGDKMSGRHG NKGTIALVCP EEDMPYLPDG RPVDICLNPM
GVPSRMNIGQ VLELHLGIAA KQLGIHVATP VFDGASEDDM WNMVREAGIG KDGKTVLYDG
RTGEPFHNRV SVGIMYYLKL THMVDDKIHA RSIGPYSLVT QQPLGGKAQF GGQRFGEMEV
WALEAYGAAY TLQEILTYKS DDVVGRVKAY EAIVKGERIP KPGVPESFRV LVKELQSLGL
DIKVLDMDHK EIELRDMDDD SNDHFNIDTL SKLAEQQEKK KLAEEAAKKD DKSAEPVDQS
DSSTSSDDKV SK
