RPOB_LACJO
ID RPOB_LACJO Reviewed; 1209 AA.
AC Q74L95;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LJ_0332;
OS Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=257314;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CNCM I-1225 / La1 / NCC 533;
RX PubMed=14983040; DOI=10.1073/pnas.0307327101;
RA Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C.,
RA Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R.,
RA Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.;
RT "The genome sequence of the probiotic intestinal bacterium Lactobacillus
RT johnsonii NCC 533.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AE017198; AAS08318.1; -; Genomic_DNA.
DR AlphaFoldDB; Q74L95; -.
DR SMR; Q74L95; -.
DR STRING; 257314.LJ_0332; -.
DR EnsemblBacteria; AAS08318; AAS08318; LJ_0332.
DR KEGG; ljo:LJ_0332; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_9; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000000581; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1209
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000224065"
FT REGION 1173..1209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1209 AA; 135814 MW; D2AB5FD4995D4857 CRC64;
MLGHAVNYGS HRTRRSFSRI KEVLKLPNLT DVQTQSYKWF LNEGIREMFD DIMPISDFSG
KLSLEFVDYK LLKPKYTLEE ARDHDANYSA PLHVTLKLTN HETGEIKTQD VFFGEFPLMT
DSGTFVINGA ERVIVSQLVR SPGVYYHSDF DKNGRQIFGA TVIPNRGAWL EYETDAKDLA
YVRIDRTRKL PLTVLIRALG FGSDSEIADM FGESDSIRFT LEKDIHKNPA DSRVAEALKD
IYERLRPGEP KTTDSSRSLL YARFFDPRRY DLAPVGRYKI NKKLSLKNRL LRQTLAETLA
DPDTGEIIAK KGDVVTHELL DKLSPYLDRD DFKMVTYEPS KEGVLPDPVT VQEIKVYSKV
DPERVVKLMS NGHIADDVKH LTPADVLASI NYFFDLQDNI GTTDDIDHLG NRRIRRVGEL
LQNQFRIGLA RMERVVRERM SIQDISTVTP QQLINIRPVV ASVKEFFGSS QLSQFMDQNN
PLGELTHKRR MSALGPGGLS RDRAGYEVRD VHYTHYGRLC PIETPEGPNI GLINSLATYA
IVNKYGFIET PYRRVSWDTH KVTDKIDYLT ADVEDNYIIA GANARLNEDG SFKDKIVLAR
HKEDNLEVTP DKIDYMDVIP KQVVSVTSAC IPFLENDDSN RALMGANHQR QAVPLINPHA
PIVGTGMEYR AAHDSGDALV AKAPGVVEYV DANEIRIRRD DDTLDKYVLE KFRRSNATKN
YNQTPAVKQG ERVVADEVIA DGPAMENGEL ALGQNPIIAF LTWNMYNYED AVMISERMVK
DDVYTSIHIE DYESEARDTK LGPEEITREI PNVGEDALKD LDEEGIVRIG AEVQDGDILV
GKVTPKGVTE LSAEERLLHA IFGEKAREVR DTSLRVPHGG GGIVQNVQVF TREAGDELPP
GVNKMVRVYI VQKRKIQVGD KMSGRHGNKG TIALVCPEED MPYLPDGRPV DICLNPMGVP
SRMNIGQVLE LHLGIAAKQL GIHVATPVFD GASEDDMWNM VREAGIGKDG KTVLYDGRTG
EPFHNRVSVG IMYYLKLTHM VDDKIHARSI GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL
EAYGAAYTLQ EILTYKSDDV VGRVKAYEAI VKGERIPKPG VPESFRVLVK ELQSLGLDIK
VLDMDHKEIE LRDMDDDSND HFNIDTLSKL AEQQEKKKLA EEAAKKDDKP DEPVDESDSS
TSSDDKVSK
