RPOB_LACLA
ID RPOB_LACLA Reviewed; 1196 AA.
AC Q9CEN6;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LL1800;
GN ORFNames=L0137;
OS Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=272623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IL1403;
RX PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA Ehrlich S.D., Sorokin A.;
RT "The complete genome sequence of the lactic acid bacterium Lactococcus
RT lactis ssp. lactis IL1403.";
RL Genome Res. 11:731-753(2001).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AE005176; AAK05898.1; -; Genomic_DNA.
DR PIR; H86849; H86849.
DR RefSeq; NP_267957.1; NC_002662.1.
DR RefSeq; WP_003130544.1; NC_002662.1.
DR AlphaFoldDB; Q9CEN6; -.
DR SMR; Q9CEN6; -.
DR STRING; 272623.L0137; -.
DR PaxDb; Q9CEN6; -.
DR PRIDE; Q9CEN6; -.
DR EnsemblBacteria; AAK05898; AAK05898; L0137.
DR KEGG; lla:L0137; -.
DR PATRIC; fig|272623.7.peg.1928; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_9; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000002196; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1196
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047907"
SQ SEQUENCE 1196 AA; 133154 MW; BB4818030426FE8A CRC64;
MAGHDVKYGK HRTRRSFSRI KEVIGLPNLI EVQTLSYKNF LDEGLANVFK EMFPIDNFAG
TMELEFVGYE MKTPKYTVEE ARAHDANYSA PIYVTFRLVN KETGELKTQE VFFGDFPLMT
EMGTFINNGS ERLIVSQLVR SPGSYFHLKA DKNGLESFGH TTIPNRGAWF ELDTDAKGIG
YVRIDRTRKL TFTTMLRALG FGSDEEILEL LGETQLLTDT IAKDVHKNPA DTRVEEALKD
IYDRLRPGEP KTADSSRGLL VARFFDPKRY DFAPVGRYKF NKKLALKNRL LGLTLAEPIV
DPETGEILVN TDTLVTRDVL DLIEPLLDNG LGNFVVEPSD DAVIPEPITL QSIKVYSPKD
PERVVTLLSN GNPDAECRVL TPADVISNIS YWLGLAEGIG KVDDIDHLGN RRIRSVGELL
QNQVRIGLSR MERVIRERMS SSENENITPQ GLINIRPVTA SIKEFFGSSQ LSQFMDQHNP
LSELSHKRRF SALGPGGISR DRASYEVRDV HYTHYGRMCP IETPEGPNIG LINNLSSYAK
VNEYGFIMSP YRRVDRVNGV VTDEVEYLTA DEEDNYTVAQ ANSPLTEDSR FANETVMARH
TGNNIEVEAS TADYMDVSPK QVIAVAAACI PFLENDDSNR ALMGANMQRQ AVPLIDPHAP
WIGTGMEHQT ARDSGAALIA KHAGVVEYVD GNEIRVRRTS GELDIYNITK YRRSNSGTSY
NQRPLARLGE KVEKGDIIAD GPSMENGEMA LGQNPLVAYM TWEGYNFEDA VIMSERLIKD
DVYTSIAIEE YESETRDTKL GPEEITREIP NVGDEALKNL DESGIIRIGA EVKDGDLLVG
KVTPKGETDP TPEERLLRAI FGEKAREVRD TSLRVPHGGG GIVHDVRVFT RENGDELPSG
VNKLVRVFIA QKRKIHVGDK MAGRHGNKGV VSNIVPMEDM PYLPDGTPID IMLNPLGVPS
RMNIGQVMEL HLGMAARTLG IHIATPVFDG ASDEDIWDTV KEAGMADDAK TVLYDGRTGE
PFDNRISVGV MYMIKLHHMV DDKLHARSVG PYSLVTQQPL GGKAQFGGQR FGEMEVWALE
AYGAANVLQE ILTYKSDDVI GRTRAYEAIV KGERIPKPGL PESFRVLVKE LQSLGLDMKV
LDADRNVLDL RELDEDEVMT RPDNTEITPE MLEAQEAIVA QAEAEEEALI NADTEK
