ID   RPOB_LACLS              Reviewed;        1196 AA.
AC   Q02X59;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=LACR_1981;
OS   Lactococcus lactis subsp. cremoris (strain SK11).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus; Lactococcus cremoris subsp. cremoris.
OX   NCBI_TaxID=272622;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SK11;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000425; ABJ73463.1; -; Genomic_DNA.
DR   RefSeq; WP_011676807.1; NC_008527.1.
DR   AlphaFoldDB; Q02X59; -.
DR   SMR; Q02X59; -.
DR   EnsemblBacteria; ABJ73463; ABJ73463; LACR_1981.
DR   KEGG; llc:LACR_1981; -.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000000240; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1196
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300336"
SQ   SEQUENCE   1196 AA;  133217 MW;  A21631ABBAACF6A3 CRC64;
     MAGHDIKYGK HRTRRSFSRI KEVIGLPNLI EVQTLSYKNF LDEGLANVFK EMFPIDNFAG
     TMELEFVGYE MKTPKYTVEE ARAHDANYSA PIYVTFRLVN KETGELKTQE VFFGDFPLMT
     EMGTFINNGS ERLIVSQLVR SPGSYFHLKT DKNGLESFGH TTIPNRGAWF ELDTDAKGIG
     YVRIDRTRKL TFTTMLRALG FGSDDEILEL LGETQLLTDT IAKDVHKNPA DTRVEEALKD
     IYDRLRPGEP KTADSSRGLL VARFFDPKRY DFAPVGRYKF NKKLALKNRL LGLTLAEPIV
     DPETGEILVN TDTLVTRDVL DLIEPLLDNG LGNFVVEPSD DAVIPEPITL QSIKVYSPKD
     SERVVTLLSN GNPDSECRVL TPADVISNIS YWLGLAEGIG KVDDIDHLGN RRIRSVGELL
     QNQVRIGLSR MERVIRERMS SSENENITPQ GLINIRPVTA SIKEFFGSSQ LSQFMDQHNP
     LSELSHKRRF SALGPGGISR DRASYEVRDV HYTHYGRMCP IETPEGPNIG LINNLSSYAK
     VNEYGFIMSP YRRVDRVNGI VTDEVEYLTA DEEDNYTVAQ ANSPLTDDSR FANETVMARH
     TGNNIEVEAS TADYMDVSPK QVIAVAAACI PFLENDDSNR ALMGANMQRQ AVPLIDPHAP
     WIGTGMEHQT ARDSGAALLA KHAGVVEYVD GNEIRVRRTS GELDIYNITK YRRSNSGTSY
     NQRPLARLGE KVEKNDIIAD GPSMENGEMA LGQNPLVAYM TWEGYNFEDA VIMSERLIKD
     DVYTSIAIEE YESETRDTKL GPEEITREIP NVGDEALKNL DESGIIRIGA EVKDGDLLVG
     KVTPKGETDP TPEERLLRAI FGEKAREVRD TSLRVPHGGG GIVHDVRVFT RENGDELPSG
     VNKLVRVFIA QKRKIHVGDK MAGRHGNKGV VSNIVPVEDM PYLPDGTPID IMLNPLGVPS
     RMNIGQVMEL HLGMAARTLG IHIATPVFDG ASDEDIWDTV KEAGMAADAK TVLYDGRTGE
     PFDNRISVGV MYMIKLHHMV DDKLHARSVG PYSLVTQQPL GGKAQFGGQR FGEMEVWALE
     AYGAANVLQE ILTYKSDDVI GRTRAYEAIV KGERIPKPGL PESFRVLVKE LQSLGLDMKV
     FDADRNVLDL RELDEDEVMT RPDNTEITPE MLEAQEAIVA QAEAEEEALI NADIEK